Freeze-drying of Lactobacillus coryniformis Si3: effects of sucrose concentration, cell density, and freezing rate on cell survival and thermophysical properties
2006 (English)In: Cryobiology, ISSN 0011-2240, E-ISSN 1090-2392, Vol. 53, no 1, 119-127 p.Article in journal (Refereed) Published
Freeze-drying is commonly used to stabilize lactic acid bacteria. Many factors have been reported to influence freeze-drying survival, including bacterial species, cell density, lyoprotectant, freezing rate, and other process parameters. Lactobacillus coryniformis Si3 has broad antifungal activity and a potential use as a food and feed biopreservative. This strain is considered more stress sensitive, with a low freeze-drying survival, compared to other commercialized antifungal lactic acid bacterial strains. We used a response surface methodology to evaluate the effects of varying sucrose concentration, cell density and freezing rate on Lb. coryniformis Si3 freeze-drying survival. The water activity of the dry product, as well as selected thermophysical properties of importance for freeze-drying; degree of water crystallization and the glass transition temperature of the maximally freeze concentrated amorphous phase (Tg') were determined. The survival of Lb. coryniformis Si3 varied from less than 6% to over 70% between the different conditions. All the factors studied influenced freeze-drying survival and the most important factor for survival is the freezing rate, with an optimum at 2.8 degrees C/min. We found a co-dependency between freezing rate and formulation ingredients, indicating a complex system and the need to use statistical tools to detect important interactions. The degree of water crystallization decreased and the final water activity increased as a function of sucrose concentration. The degree of water crystallization and Tg' was not affected by the addition of 10(8)-10(10) CFU/mI. At 10(11) CFU/ml, these thermophysical values decreased possibly due to increased amounts of cell-associated unfrozen water.
Place, publisher, year, edition, pages
Maryland Heights, USA: Elsevier, 2006. Vol. 53, no 1, 119-127 p.
Lactic acid bacteria, Lactobacillus coryniformis, freezing, degree of water crystallization, Tg, water activity, optimization, product quality
IdentifiersURN: urn:nbn:se:oru:diva-52380DOI: 10.1016/j.cryobiol.2006.04.003ISI: 000239479200012PubMedID: 16756971ScopusID: 2-s2.0-33745809453OAI: oai:DiVA.org:oru-52380DiVA: diva2:972105