Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121
2007 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 73, no 5, 1008-1016 p.Article, review/survey (Refereed) Published
The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations.
Place, publisher, year, edition, pages
New York, USA: Springer, 2007. Vol. 73, no 5, 1008-1016 p.
Pichia anomala, biocontrol, formulation, shelf-life, trehalose
IdentifiersURN: urn:nbn:se:oru:diva-52378DOI: 10.1007/s00253-006-0552-xISI: 000242968300004PubMedID: 16933132ScopusID: 2-s2.0-33845790664OAI: oai:DiVA.org:oru-52378DiVA: diva2:972116