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Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
2007 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 73, no 5, 1008-1016 p.Article, review/survey (Refereed) Published
Abstract [en]

The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations.

Place, publisher, year, edition, pages
New York, USA: Springer, 2007. Vol. 73, no 5, 1008-1016 p.
Keyword [en]
Pichia anomala, biocontrol, formulation, shelf-life, trehalose
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:oru:diva-52378DOI: 10.1007/s00253-006-0552-xISI: 000242968300004PubMedID: 16933132Scopus ID: 2-s2.0-33845790664OAI: oai:DiVA.org:oru-52378DiVA: diva2:972116
Available from: 2016-09-20 Created: 2016-09-19 Last updated: 2017-10-17Bibliographically approved

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CiteExportLink to record
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