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In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
The Institute of Protein Environment Affinity Surveys (PEAS Institut), Linköping, Sweden; Maternal and Children Health Care Hospital of Zhuhai City, Zhuhai, China .
Örebro University, School of Medical Sciences. The Institute of Protein Environment Affinity Surveys (PEAS Institut), Linköping, Sweden .
The Institute of Protein Environment Affinity Surveys (PEAS Institut), Linköping, Sweden .
Örebro University, School of Medical Sciences.
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2016 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 16, no 1, 209Article in journal (Refereed) Published
Abstract [en]

Background: There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host’s defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. Current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. The study aims to develop anti-bacterial human antibodies in vitro from patients who had recently undergone a systemic infection by pathogenic bacteria and to use these antibodies as a tool for detecting bacteria in biofilms.

Methods: Lymphocytes were separated from whole blood of patients (n = 10) and stimulated with heat-killed bacteria to produce antibodies in vitro. The specificity of antibodies in recognizing the bacteria against which they were directed was evaluated by surface plasmon resonance system (SPR) and electron microscopy. The ulcer secretions from patients with chronic and acute leg ulcers and healthy controls were analyzed by the SPR system and the results were compared with culture studies.

Results: The produced antibodies recognized bacteria with high sensitivity (SPR). The antibodies against Enterococcus fecalis bound specifically to the microorganism in a bacterial co-culture that was visualized by electron microscopy.

Conclusion: In the present work, a method for producing specific antibodies against bacteria is introduced to recognize bacterial components in body fluids of patients suffering from pathogenic biofilms. This diagnostic technique may be most useful in clinical microbiology and in the choice of antibiotics in the treatment of serious infections.

Place, publisher, year, edition, pages
London, United Kingdom: BioMed Central, 2016. Vol. 16, no 1, 209
Keyword [en]
Enterococcus, Biofilms, Antibodies, Detection, Electron microscopy, Lymphocytes, Surface plasmon resonance
National Category
Infectious Medicine Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-52181DOI: 10.1186/s12866-016-0821-5ISI: 000383425300002PubMedID: 27612600ScopusID: 2-s2.0-84986905110OAI: oai:DiVA.org:oru-52181DiVA: diva2:972623
Note

Funding Agencies:

PEAS Institut

FORSS F2004-212

ALF grants LIO-206241

Available from: 2016-09-21 Created: 2016-09-14 Last updated: 2016-11-15Bibliographically approved

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