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  • 1.
    Feng, Xin Mei
    et al.
    Department of Microbiology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
    Passoth, Volkmar
    Department of Microbiology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
    Eklund-Jonsson, Charlotte
    Department of Chemical and Biological Engineering, Food Science, Chalmers University of Technology, Göteborg, Sweden.
    Alminger, Marie Larsson
    Department of Chemical and Biological Engineering, Food Science, Chalmers University of Technology, Göteborg, Sweden.
    Schnürer, Johan
    Department of Microbiology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
    Rhizopus oligosporus and yeast co-cultivation during barley tempeh fermentation: Nutritional impact and real-time PCR quantification of fungal growth dynamics2007Inngår i: Food microbiology (Print), ISSN 0740-0020, E-ISSN 1095-9998, Vol. 24, nr 4, s. 393-402Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Barley tempeh was produced by fermenting barley kernels with Rhizopus oligosporus. The potential of the yeasts Saccharomyces cerevisiae (three strains), S. boulardii (one strain), Pichia anomala (one strain) and Kluyveromyces lactis (one strain) to grow together with R. oligosporus during barley tempeh fermentation was evaluated. All yeast strains grew during the fermentation and even during cold storage of tempeh (P < 0.01). The growth of yeasts slightly increased the ergosterol contents, but did not influence amino acid contents and compositions, and did not reduce phytate contents. Slight increases of vitamins B-6 and niacinamide, and slight decreases of B, and biotin were observed. Quantification of fungal growth is difficult during mixed species fermentations because ergosterol is found in all fungal species, and colony-forming-unit (cfu) estimations are not reliable for R. oligosporus and other sporulating fungi. Therefore, we developed a quantitative real-time PCR method for individually quantifying S. cerevisiae and R. oligosporus growth in barley tempeh. The PCR results were highly correlated with the ergosterol content of R. oligosporus and with the number of cfu of S. cerevisiae. Thus, real-time PCR is a rapid and selective method to quantify yeasts and R. oligosporus during mixed species fermentation of inhomogenous substrate such as barley tempeh.

  • 2.
    Loncarevic, Semir
    et al.
    Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Bannerman, Elisabeth
    Centre National des Listeria, Institut de Microbiologi, Lausanne, Switzerland.
    Bille, Jacques
    Centre National des Listeria, Institut de Microbiologi, Lausanne, Switzerland.
    Danielsson-Tham, Marie-Louise
    Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Tham, Wilhelm
    Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Characterization of Listeria strains isolated from soft and semi-soft cheeses1998Inngår i: Food microbiology (Print), ISSN 0740-0020, E-ISSN 1095-9998, Vol. 15, nr 5, s. 521-525Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Two hundred strains ofListeria monoctyogenespreviously isolated from 19 soft and semi-soft cheeses by enrichment were characterized by serotyping and pulsed-field gel electrophoresis (PFGE). Strains of serogroup 1/2 predominated, with 33.5% of all strains belonging to serovar 1/2a, 58.5% to serovar 1/2b and 5% to serovar 1/2c. By using a PFGE method, 16 different clonal types were obtained. All 10 isolates ofL. monocytogenesrecovered from one white mould cheese were serovar 1/2a, but displayed two different PFGE profiles. Another 10L. monocytogenesisolates from white mould cheese recovered after a 48-h enrichment broth procedure revealed two clonal types, only one of which could be detected after 5 additional days of incubation in the same enrichment broth. Cross-contamination in dairy plants and/or retail stores may play an important role in the incidence and diversity ofL. monocytogenesclonal types recovered from soft and semi-soft cheeses. Therefore, it is necessary to characterize several isolates from the same sample when conducting epidemiological investigations.

    Copyright © 1998 Academic Press. All rights reserved.

  • 3.
    Loncarevic, Semir
    et al.
    Department of Food Hygiene, Faculty of Veterinary Medicine SLU, Uppsala, Sweden.
    Danielsson-Tham, Marie-Louise
    Department of Food Hygiene, Faculty of Veterinary Medicine SLU, Uppsala, Sweden.
    Gerner-Smidt, Peter
    National Serum Institute, Department of Gastrointestinal Infections, Copenhagen, Denmark.
    Sahlström, Lena
    Department of Food Hygiene, Faculty of Veterinary Medicine SLU, Uppsala, Sweden.
    Tham, Wilhelm
    Department of Food Hygiene, Faculty of Veterinary Medicine SLU, Uppsala, Sweden.
    Potential sources of human listeriosis in Sweden1998Inngår i: Food microbiology (Print), ISSN 0740-0020, E-ISSN 1095-9998, Vol. 15, nr 1, s. 65-69Artikkel i tidsskrift (Fagfellevurdert)
  • 4.
    Tham, Wilhelm
    et al.
    Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Hajdu, Lajos
    Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    A comparison of six media for isolating Staphylococcus aureus from foods1987Inngår i: Food microbiology (Print), ISSN 0740-0020, E-ISSN 1095-9998, Vol. 4, nr 2, s. 133-146Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Six culture media were compared with blood agar regarding colony counts and colony diameters of 53 Staphylococcus aureus strains. There were no statistically significant differences between the media. The forced differentiation of the results via cluster analysis, however, gave some indications that differences existed. In terms of colony counts, LSM, BP Oxoid and BP Oxoid + pp gave results similar to those given by blood agar. Colony diameter seemed to be a doubtful measure of the media's suitability and none of the six media showed similar diameter values to those of blood agar. As regards the appearance of the S. aureus strains on the six media, BP BBL and LSM corresponded the most closely to the inventors descriptions. The selectivities of the media were tested by cultivating 102 food samples from various sources. The most favourable retardation against micro-organisms other than S. aureus was shown by BP BBL, KRANEP and LSM. In terms of all tests performed, BP BBL was the most satisfactory medium for isolating S. aureus. PCVJ was the poorest of all media used in this study.

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