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  • 1.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Sartz, Lotta
    Örebro University, School of Science and Technology. Bergskraft Bergslagen AB, Kumla, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Sädbom, Stefan
    Bergskraft Bergslagen AB, Kumla, Sweden.
    Control of metal releases from historic sulphidic mine waste: Experience from the test site at the Ljusnarsberg mine field, Sweden (Project Bergskraft Bergslagen)2010In: Proc. EU Mine Drainage Research Exchange Conf. PADRE, June 11, Freiberg, Germany, 2010, p. 1 p-Conference paper (Refereed)
  • 2.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Black shale: a biogeochemical archive2014In: Sedimentary Pore Space Cementation: Role of Microbes / [ed] Kothe E, Büchel G, 2014, p. 6-Conference paper (Refereed)
  • 3.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology. Remedy by Sweden AB, Eskilstuna, Sweden; Mälardalen Univ., Västerås, Sweden.
    Hedlund, Jonas
    Structor Miljöteknik AB, Eskilstuna, Sweden.
    Carlsson, Peter
    Remedy by Sweden AB, Eskilstuna, Sweden; Structor Miljöteknik AB, Eskilstuna, Sweden.
    Eriksson, Ingvar
    Remedy by Sweden AB, Eskilstuna, Sweden; Structor Miljöteknik AB, Eskilstuna, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology. Remedy by Sweden AB, Eskilstuna, Sweden.
    Metal mobility or metal concentration as the basis for remediation strategy: a case study2014Conference paper (Refereed)
  • 4.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Metal loads or metal concentrations as the basis for risk assessment of a polluted site: a case study2013In: Sardinia 2013: executive summaries : proceedings of the fourteenth International Waste Management and Landfill Symposium / [ed] Raffaello Cossu, Pinjing He, Peter Kjeldsen, Yasushi Matsufuji, Debra Reinhart, Rainer Stegmann, Cagliari: CISA , 2013Conference paper (Refereed)
  • 5.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology. Remedy by Sweden AB, Eskilstuna, Sweden; Mälardalen Univ., Västerås, Sweden.
    Martell, Ulrika
    Structor Miljöteknik AB, Eskilstuna, Sweden.
    Andersson, Matilda
    Structor Miljöteknik AB, Eskilstuna, Sweden.
    Nordén, Anna
    Structor Miljöteknik AB, Eskilstuna, Sweden.
    Karlsson, Stefan
    Örebro University, School of Science and Technology. Remedy by Sweden AB, Eskilstuna, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Carlsson, Peter
    Remedy by Sweden AB, Eskilstuna, Sweden; Structor Miljöteknik AB, Eskilstuna, Sweden.
    Reduction in situ of chromium(VI) at a heavily polluted site: a feasible remediation strategy2014Conference paper (Refereed)
  • 6.
    Allard, Bert
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Susanne
    Department of Geological Sciences, Stockholm University, Stockholm, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Skogby, Henrik
    Department of Geosciences, Swedish Museum of Natural History, Stockholm, Sweden.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Metal Exchangeability in the REE-Enriched Biogenic Mn Oxide Birnessite from Ytterby, Sweden2023In: Minerals, E-ISSN 2075-163X, Vol. 13, no 8, article id 1023Article in journal (Refereed)
    Abstract [en]

    A black substance exuding from fractures was observed in 2012 in Ytterby mine, Sweden, and identified in 2017 as birnessite with the composition Mx[Mn(III,IV)](2)O-4 center dot(H2O)n. M is usually calcium and sodium, with x around 0.5. The Ytterby birnessite is unique, with M being calcium, magnesium, and also rare earth elements (REEs) constituting up to 2% of the total metal content. The biogenic origin of the birnessite was established in 2018. Analysis of the microbial processes leading to the birnessite formation and the REE enrichment has continued since then. The process is fast and dynamic, as indicated by the depletion of manganese and of REE and other metals in the fracture water during the passage over the precipitation zone in the mine tunnel. Studies of the exchangeability of metals in the structure are the main objective of the present program. Exposure to solutions of sodium, calcium, lanthanum, and iron led to exchanges and altered distribution of the metals in the birnessite, however, generating phases with almost identical structures after the exchanges, and no new mineral phases were detected. Exchangeability was more efficient for trivalent elements (REE) over divalent (calcium) and monovalent (sodium) elements of a similar size (ionic radii 90-100 pm).

  • 7.
    Chupani, Latifeh
    et al.
    The Life Science Centre—Biology, School of Science and Technology, Örebro University, Örebro, Sweden; South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Jass, Jana
    Örebro University, School of Science and Technology.
    Olsson, Per-Erik
    Örebro University, School of Science and Technology.
    Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna2022In: Fishes, E-ISSN 2410-3888, Vol. 7, no 5, article id 248Article in journal (Refereed)
    Abstract [en]

    The influence of water hardness on copper (Cu) toxicity in Daphnia magna was studied using gene expression analysis. Exposing D. magna to Cu in water with increasing levels of hardness decreased the acute toxicity. Hardness did not affect the predicted Cu complexation. After 24 h, D. magna showed an increased level of genes related to metal homeostasis (mt) following exposure to 25 mu g Cu/L in hard water. Daphnids in soft and medium water responded to 25 mu g Cu/L by upregulation of antioxidant defense and mt genes, revealing oxidative stress as a mechanism of Cu toxicity in D. magna. D. magna exposed to 25 mu g Cu/L in soft water did not survive for 96 h. In contrast, those exposed to 25 mu g Cu/L in medium and hard water survived for 96 h with significantly higher levels of mt genes. The genes related to oxidative damage (heat shock protein and glutathione S-transferase) in these groups did not deviate from control levels, indicating the protective effect of hardness. Metallothionein genes were upregulated at 17 mu g Cu/L at both 24 h and 96 h. The expression of catalase and ferritin increased in this group in soft and hard water at 96 h. The protective effect of hardness (in the tested range) on survival was also observed at a concentration of 25 mu g/L. The results suggest metallothionein (A and B), catalase, and ferritin genes, as potential biomarkers for copper exposure in D. magna regardless of hardness.

  • 8.
    Dubocq, Florian
    et al.
    Örebro University, School of Science and Technology.
    Wang, Thanh
    Örebro University, School of Science and Technology.
    Yeung, Leo W. Y.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Kärrman, Anna
    Örebro University, School of Science and Technology.
    Characterization of the Chemical Contents of Fluorinated and Fluorine-Free Firefighting Foams Using a Novel Workflow Combining Nontarget Screening and Total Fluorine Analysis2020In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 54, no 1, p. 245-254Article in journal (Refereed)
    Abstract [en]

    Aqueous film-forming foams (AFFFs) are widely used to extinguish liquid fires due to their film-forming properties. AFFF formulation historically contains per- and polyfluoroalkyl substances (PFASs) that can be very persistent and pose a health risk to biota and humans. Detailed analysis of the chemical composition of AFFFs can provide a better understanding on the potential environmental impact of the ingredients. In this study, a novel workflow combining target analysis, nontarget screening analysis (NTA), total fluorine (TF) analysis, and inorganic fluoride (IF) analysis was applied to disclose the chemical composition of 24 foams intended for liquid fires. Foams marketed as containing PFASs as well as fluorine-free foams were included. By comparing the sum of targeted PFASs and total organofluorine concentrations, a mass balance of known and unknown organofluorine could be calculated. Known organofluorine accounted for <1% in most fluorine-containing AFFFs, and it was confirmed that the foams marketed as fluorine-free did not contain measurable amounts of organofluorine substances. Five fluorinated substances were tentatively identified, and non-fluorinated zwitterionic betaine compounds, which are considered to be replacement substances for PFASs, were tentatively identified in the organofluorine-free foams.

  • 9.
    Grandin, Anna
    et al.
    Örebro University, School of Science and Technology.
    Ogar, Anna
    Nilsson, Charlotte
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Retention of uranium (VI) by live fungal biomass from a uranyl nitrate solution: Implications and applications under nutrient-poor conditionsManuscript (preprint) (Other academic)
  • 10. Grandin, Anna
    et al.
    Ogar, Anna
    Institute of Environmental Science, Jagiellonian University, Krakow, Poland.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Potential use of native fungal strains for assisted uranium retention2015In: Minerals Engineering, ISSN 0892-6875, E-ISSN 1872-9444, Vol. 81, p. 173-178Article in journal (Refereed)
    Abstract [en]

    Uranium-stabilizing ligands can be useful complexing agents for uranium in aqueous solution. The discovery of novel ligand candidates for selective uranium capture in artificial and natural waters could provide scope for their use in water remediation and metal recovery from low- and high grade ores. In this study we used seven fungal strains, isolated from shale waste, to monitor the uranium retention capacity from an aqueous solution. After four weeks of incubation, suspensions containing the fungal strains were filtered, and up to 100% of the total uranium inventory was removed from a 10 mg L-1 solution. Approximately 70% of the total uranium removal is attributed to complexation and/or adsorption by particles in the malt extract and some 10% is adsorbed by the fungal biomass. The additional 20% uranium removed could be related to the excretion of fungal metabolites. From 58% to 90% of the uranium is removed within ten minutes. The formation of colloidal/particulate uranium is proposed to be controlled by organic ligands in the culture medium and organic ligands excreted by the fungi where phosphorus moieties seem to be important. Membrane fouling by the hydrocarbons is also suggested to contribute to a loss of uranium from the aqueous phase.

  • 11.
    Grandin, Anna
    et al.
    Örebro University, School of Science and Technology.
    Ogar, Anna
    Jagiellonian University, Krakow, Poland. .
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Biosorption of uranium by fungi isolated from weathered alum shale residues2013In: / [ed] E. Kothe and G. Büchel, 2013Conference paper (Refereed)
  • 12.
    Grandin, Anna
    et al.
    Örebro University, School of Science and Technology.
    Ogar, Anna
    Jagellonian University, Krakow, Poland.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Uranium induced stress promotes fungal excretion of uranium/metal stabilizing ligands: Analysis of metal-organic compounds with Size Exclusion Chromatography and Inductively Coupled Plasma-Mass Spectroscopy2014In: Uranium - Past and Future Challenges: Proceedings of the 7th International Conference on Uranium Mining and Hydrogeology, Springer Publishing Company, 2014, p. 347-354Conference paper (Refereed)
  • 13.
    Grandin, Anna
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Mobilization of vanadium from LD slag by salt-roasting/alkaline heterotrophic leaching2011In: 25th International Applied Geochemistry Symposium, 22-26 August, 2011, Rovaniemi, Finland: Final Programme and Abstracts / [ed] Pertti Sarala, V. Juhani Ojala, Marja-Leena Porsanger, Vuorimiesyhdistys , 2011, p. 135-136, article id P19Conference paper (Refereed)
  • 14.
    Grandin, Anna
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Stability of weathered shales at water field capacity in the presence of Aspen wood shavings2011In: Mine water: managing the challenges:  proceedings of the International Mine Water Association Congress 2011 / [ed] Trude R.Rüde, Antje Freund, Christian Wolkersdorfer, Aachen: RWTH , 2011, p. 331-335Conference paper (Refereed)
  • 15.
    Grawunder, Anja
    et al.
    Friedrich Sciller Univ., Jena, Germany.
    Schäffner, Franziska
    Friedrich Sciller Univ., Jena, Germany.
    Merten, Dirk
    Friedrich Sciller Univ., Jena, Germany.
    Büchel, Georg
    Friedrich Sciller Univ., Jena, Germany.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Rare earth elements distribution and fractionation in a former acidic shale pit lake2011In: / [ed] E. Kothe, G. Büchel, 2011, p. 44-44Conference paper (Refereed)
  • 16. Gärdek, Oskar
    et al.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Retention of metals by optimized steel slag2017In: Bio-geo interactions: basic knowledge to application: 16th Symposium on remediation in Jena “Jenaer Sanierungskolloquium”. Conference proceedings, 2017, p. 24-24Conference paper (Other academic)
    Abstract [en]

    Silicon reduced Argon Oxygen Decarburization slag (Si-AOD) is a common by-product from manufacturing of stainless steel. In this work the possibility to use the slag as adsorbent for some selected metals (Cr, Ni, Cu, Zn and Pb) is tested. The slag was obtained from Outokumpu, Avesta, Sweden as one black, powder like, and one greenish, pumice-stone like. Due to its small grain size the black Si-AOD was not suitable as a filter material since it quickly clogged and formed almost impermeable layers. However, the greenish Si-AOD with its porous structure showed good permeability and seems to be good as a filter material. Its ability to remove the selected elements from an aqueous solution was tested at different metal concentrations, ionic strengths and pH. The absorption capacity was tested in columns each holding 30 gram of the greenish Si-AOD and 300 mL of the aqueous phase was passed through the material. Six fractions of 50 mL each were collected and analyzed with respect to pH and metal content to estimate the retention capacity. With an initial concentration of 10 mg/L (Ni, Cu and Zn) and 1 mg/L (Cr and Pb) an almost 100% adsorption was obtained. The adsorption seemed to follow the Freundlich adsorption isotherm and with a 1/n-value of 0.13 the adsorption process is probably chemical. The adsorption capacity of the surface was estimated to be at least 80 μeqv/g. Hence a liquid to solid ratio of up to 100 can be reached with the used metal solution before the retention capacity of the slag is depleted

  • 17.
    Karlsson, Lovisa
    et al.
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Release of metals from unprocessed and processed black shale due to natural weathering2013In: Reliable Mine Water Technology. Volume 1: Proceedings of the International Mine Water Association Annual Conference 2013 / [ed] Adrian Brown; Linda Figueroa; Christian Wolkersdorfer, Colorado, USA: IMWA , 2013, p. 391-397Conference paper (Refereed)
    Abstract [en]

    Black shale was mined and processed for recovery of hydrocarbons in Kvarntorp, some 200 km SW of Stockholm, Sweden, during 1942–66. Remains from the mining period is a deposit with 40 Mm³ of crushed shale residues: Unprocessed 3ne-grained shale as well as processed shale. The deposit is still hot; oxidation of sulphides as well as burning of hydrocarbons are still in progress some 50 years a1er closure. Weathering of the shale leads to releases of metal-rich leachates which will increase with time. The release of metals from pristine shale as well as weathered shale (exposed to the atmosphere for 50 years) and two di2erent processed shale residues have been studied using water of di2erent pH as leaching solution, to simulate extreme pH-variations in environmental waters: 3.0 (determined by oxidation of iron sulphides), 5.5 (bu2ered by carbon dioxide), 8.5 (bu2ered by calcite) and 12.5 (bu2ered by calcium hydroxide). Extraction of cationic elements was substantial (several percent of the total content) for Ca and Mg, as well as Ni, Co and U at pH 3, and very high (up to 50–60 %) for anionic elements (V, Mo, As) at pH 12.5. Especially the high-temperature processed shale would be a potential source for As and Mo at high pH. The pH-dependence of the weathering processes should be considered when future remediation (or metal recovery) processes and strategies are designed.

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  • 18. Karlsson, Lovisa
    et al.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Bark compost for removal of Nickel in complex waste water2012In: / [ed] E. Kothe and G. Büchel, 2012Conference paper (Refereed)
  • 19.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Concentration dynamics in a boreal catchment: trace elements, REEs and humic substances2011In: 25th International Applied Geochemistry Symposium, 22-26 August, 2011, Rovaniemi, Finland / [ed] Pertti Sarala, V. Juhani Ojala, Marja-Leena Porsanger, Rovaniemi, Finland: Vuorimiesyhdistys - Finnish Association of Mining and Metallurgical Engineers , 2011, p. 63-63Conference paper (Refereed)
  • 20.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Karlsson, Lovisa
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Water quality in a black shale mining area: effects of pH and natural organic acids on weathering and subsequent metal releases2014In: An Interdisciplinary Response to Mine Water Challenges / [ed] Wanghua Sui, Yajun Sun and Changshen Wang, China University of Mining and Techno logy Press , 2014, p. 136-136Conference paper (Refereed)
    Abstract [en]

    Mining of black shale (alum shale) of Late Cambrium age for the extraction of alum started in the early 17th century in Sweden, and in the late 19th century there was an extensive use of shale, with its high content of organic carbon, as fuel in lime-burning processes. Full-scale recovery of hydrocarbons from shale took place in Kvarntorp during 1942-66, and recovery of uranium in Ranstad during 1965-76. Remains from the historic mining and processing of black shale are some 50 major deposits with processed and unprocessed shale residues, as well as water-filled pits, at the former mining sites. There are also large areas covered with crushed shale, e.g. as road filling material. Studies of the weathering of shale due to exposure to the atmosphere and water with pH within the natural range indicate a high leachability of cationic elements at low pH, as well as anionic elements at high pH. The presence of organic complexing acids of natural origin (microbial exudates and humic acids) may give an enhanced leaching, particularly at pH above 8, when hydroxy-groups would constitute active metal binding sites. Results from studies of weathering and leaching of processed and unprocessed black shale are presented, as well as the effects on the water quality observed at former mining sites and related environmental impact.

  • 21.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Nilsson, Charlotte
    Örebro University, School of Science and Technology.
    Berg, Isabelle
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Avloppsslam som vattenreningsfilter2015Conference paper (Other academic)
  • 22.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Analysis of Acid Rock Drainage (ARD) with Micro Plasma Atomic Emission Spectroscopy (MP AES) – Comparison with ICP-MS2014In: Annual International Mine Water Association Conference: An Interdisciplinary Response to Mine Water Challenges / [ed] Wanghua Sui; Yajun Sun; Changshen Wang, Xuzhou: China University of Mining and Technology Press , 2014, p. 131-135Conference paper (Refereed)
    Abstract [en]

    Analysis of dissolved metals in acid rock drainage (ARD) with an Agilent 4100 MP AES instrument that combines a nitrogen micro plasma with atomic emission detection (MP AES) was compared with an ICP-MS. Sample preparation consisted only of filtration, acidification (HNO3 1%) and addition of internal standard elements after appropriate dilution. In these complex matrices the systems gave identical results provided that care was taken to avoid ionization. This was easily done by addition of CsNO3 which eliminated the need for matrix matching of calibration solutions. The use of internal standards is only needed for elements with known spectral interferences.

  • 23.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Influence of humic substances on metal mobility in soil from the Gessenwiese test field2014In: / [ed] E. Kothe and G. Büchel, 2014Conference paper (Refereed)
  • 24.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Impact of humic substances on the transport of metals from a boreal watershed: Time trends and annual variability2015Conference paper (Other academic)
  • 25.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Impact of humic substances on uranium mobility in soil: a case study from the Gessenwiese test field2014In: Uranium - Past and Future Challenges: Proceedings of the 7th International Conference on Uranium Mining and Hydrogeology / [ed] Broder J. Merkel, Alireza Arab, Cham: Springer Berlin/Heidelberg, 2014, p. 239-248Conference paper (Refereed)
  • 26.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Metal transport dynamics in a small watershed - Dylta bruk, Sweden2017In: Bio-geo interactions: basic knowledge to application: 16th Symposium on remediation in Jena “Jenaer Sanierungskolloquium”. Conference proceedings, 2017, p. 23-23Conference paper (Other academic)
    Abstract [en]

    Metal transport in small streams in boreal catchments is a function of weathering rate, water balance and redistribution mechanisms. Because of these highly dynamic processes long term water quality changes are difficult to determine but needed in order to assess the impact of several local and largescale changes on local water quality.

    The field site is situated at Dyltabruk, some 20 km North of Örebro in South Central Sweden. The 4 km2 catchment has deciduous and coniferous species on a granitic moraine with some 20% ofmires and fens adjacent to the oldest sulphur mine in Sweden. Grab samples were collected weekly since 2006 but more frequent during periods with large changes in water balance. The samples were analysed for general hydrochemical parameters (temperature, electrical conductivity, pH, dissolved organic carbon (DOC), inorganic carbon (IC), fulvic and humic acids and dissolved oxygen), dissolved principal anions, principal and trace metals. Standardized analytical procedures were applied. Temperature, precipitation and other climatic parameters were recorded some 2 km from thesite every 15 minutes.

    The results showed a general concentration pattern where the water balance had the largest single influence. The concentrations had a seasonality inversely related to the ground water level. Inter annual variations of one to two orders of magnitude were observed for group 1 and 2 elements. For transition metals with high affinity to solid matter as well as DOC the variation reached three to four orders of magnitude. Only aluminium and iron had concentrations that occasionally exceeded solubility limits which resulted in a similar inter annual variation.

    During the study period the average annual temperature and precipitation were no different (p 0.05) from the previous ten years but rainfall intensities increased. In a long term perspective the concentrations for all metals except calcium had positive trends. The tendencies remained when normalizing against chloride. The same was found for DOC, nitrate and sulphate. Hence, there is an accelerating loss of most elements that is not limited by weathering. It is uncertain, however, if the positive trends for DOC depend on increased production or a balancing release from the supply in mires and fens. In addition, there is also an indication of increasing inter annual concentration changescwith time. Although not exclusively proven such phenome would occur as a result of increased rainfall intensity. It is therefore likely that the accelerating loss of elements is a result of increased weathering rather than increasing water discharge.

  • 27.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Heterotrophic leaching of LD-slag: formation of organic ligands2011In: Mine Water: Managing the Challenges: proceedings of the International Mine Water Association Congress 2011 / [ed] Trude R.Rüde, Antje Freund, Christian Wolkersdorfer, Aachen: RWTH , 2011, p. 371-374Conference paper (Refereed)
  • 28.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Revegetating acidic mine waste using UMBRELLA guidelines: The second summer2012In: 11 Symposium on remediation in Jena, "Jenaer Sanierungskolloquium": Geobiotechnology: from lost areas to resources: Conference Proceedings, 2012Conference paper (Refereed)
  • 29.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Leaching pattern of metals from historic sulphidic mine waste upon addition of bark compost2013In: Annual International Mine Water Association Conference: Relialbe Mine Water Technology, Vol.1 / [ed] Adrian Brown; Linda Figueroa; Christian Wolkersdorfer, IMWA & QuarkXPress , 2013, p. 625-632Conference paper (Refereed)
  • 30.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Revegetation of historic acid mine waste with Agrostis capillaris: Impact on leachate composition in pot experiments2012In: International Mine Water Association Symposium / [ed] McCullough C D; Lund M A; Wyse L, Perth: IMWA & The Expo Group , 2012, p. 489-497Conference paper (Refereed)
  • 31.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Revegetation of historic acidic mine waste with Agrostis capillaris: Remediation strategy2012In: International Mine Water Association Symposium / [ed] McCullough, C.D.; Lund, M.A. & Wyse, L., International Mine Water Association ( IMWA ) , 2012, p. 317-325Conference paper (Refereed)
    Abstract [en]

    To establish vegetation on historical acidicmine waste and metal polluted soil has several environmental, physical and chemical advantages on metalrelease. Pot experiments with material from the Ljusnarsbergs mine waste deposit in Sweden showed that different kinds of refuse can be used to sustain germination and improve growth of Agrostiscapillariswhere it otherwise isimpossible. Bark compost increased the water holding capacity and an increase of pHin the top 50 mm by addition of water works granules made it possible for the grass to survive. An even better growth was obtained if those systems were also inoculated with mycorrhiza.

  • 32.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Substrate conditioning for growth of Agrostis capillaris on historical sulphidic mine waste: Impact on ARD composition2013Conference paper (Refereed)
  • 33.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Ogar, Anna
    Plant-Microbial Interactions, Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland.
    Comparison of MP AES and ICP-MS for analysis of principal and selected trace elements in nitric acid digests of sunflower (Helianthus annuus)2015In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 135, p. 124-132Article in journal (Refereed)
    Abstract [en]

    The use of nitrogen as plasma gas for microwave plasma atomic emission spectroscopy (MP AES) is an interesting development in analytical science since the running cost can be significantly reduced in comparison to the inductively coupled argon plasma. Here, we evaluate the performance of the Agilent 4100 MP AES instrument for the analysis of principal metals (Ca, K, Mg, and Na), lithogenic metals (Al, Fe, and Mn) and selected trace metals (As, Ba, Cd, Co, Cr, Cu, Mo, Ni, Pb, V, and Zn) in nitric acid plant digests. The digests were prepared by microwave-assisted dissolution of dry plant material from sunflower (Helianthus annuus) in concentrated nitric acid. Comparisons are made with analysis of the same solutions with ICP-MS (Agilent 7500cx) using the octopole reaction system (ORS) in the collision mode for As, Fe, and V.

    The limits of detection were usually in the low μg L-1 range and all principal and lithogenic metals were successfully determined with the MP AES and provided almost identical results with the ICP-MS. The same applies for the selected trace metals except for As, Co and Mo where the concentrations were below the detection limit with the MP AES. For successful analysis we recommend that (i) only atom lines are used, (ii) ionization is minimized (e.g. addition of CsNO3) and (iii) the use of internal standards should be considered to resolve spectral interferences.

  • 34.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Ogar, Anna
    Dep. Environ. Sci., Jagellonian University, Krakow, Poland.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Leachability of Cu, Zn, As, Ba and Pb from refuse in the Zelazny Most tailings dam2014In: Annual International Mine Water Association Conference: An Interdisciplinary Response to Mine Water Challenges / [ed] Sui, W., Sun, Y. & Wang, C., Xuzhou, China: China University of Mining and Technology Press , 2014, p. 121-125Conference paper (Refereed)
    Abstract [en]

    In order to establish plants on any kind of mine waste it is essential to determine its chemical properties, i.e. the potential to release elements. In this study we combine chemical extraction of six different solid metal species (water soluble, ion-exchangeable, carbonate, reducible, oxidizable acid leachable) on tailings from Zelazny Most, Poland. The results show that the plants encounter an environment with circumneutral pH and high availability of Ca, Na, and Cu. During reducing conditions there is also an increasing availability of As, Fe, Pb, and Zn.

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  • 35.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Pourjabbar, Anahita
    Friedrich Schiller Univ., Jena, Germany.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Distribution of rare earth elements and other metals in a stratified acidic pit lake in black shales 45 years after mine closure2012In: 9th International Conference on Acid Rock Drainage  (ICARD 2012): Ottawa, Canada20-26 May 2012 / [ed] Price WA, Hogan C, Tremblay G, Curran Associates, Inc., 2012, p. 812-821Conference paper (Refereed)
  • 36.
    Karlsson, Stefan
    et al.
    Örebro University, School of Science and Technology.
    Wetterholm, Petter
    Structor Miljöteknik AB, Västerås, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Allard, Bert
    Örebro University, School of Science and Technology.
    Chemical characterization of metal polluted soils: why and how2014Conference paper (Refereed)
  • 37.
    Kurtser, Polina
    et al.
    Örebro University, School of Science and Technology.
    Castro Alves, Victor
    Örebro University, School of Science and Technology.
    Arunachalam, Ajay
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Hanell, Ulf
    Örebro University, School of Science and Technology.
    Hyötyläinen, Tuulia
    Örebro University, School of Science and Technology.
    Andreasson, Henrik
    Örebro University, School of Science and Technology.
    Development of novel robotic platforms for mechanical stress induction, and their effects on plant morphology, elements, and metabolism2021In: Scientific Reports, E-ISSN 2045-2322, Vol. 11, no 1, article id 23876Article in journal (Refereed)
    Abstract [en]

    This research evaluates the effect on herbal crops of mechanical stress induced by two specially developed robotic platforms. The changes in plant morphology, metabolite profiles, and element content are evaluated in a series of three empirical experiments, conducted in greenhouse and CNC growing bed conditions, for the case of basil plant growth. Results show significant changes in morphological features, including shortening of overall stem length by up to 40% and inter-node distances by up to 80%, for plants treated with a robotic mechanical stress-induction protocol, compared to control groups. Treated plants showed a significant increase in element absorption, by 20-250% compared to controls, and changes in the metabolite profiles suggested an improvement in plants' nutritional profiles. These results suggest that repetitive, robotic, mechanical stimuli could be potentially beneficial for plants' nutritional and taste properties, and could be performed with no human intervention (and therefore labor cost). The changes in morphological aspects of the plant could potentially replace practices involving chemical treatment of the plants, leading to more sustainable crop production.

  • 38.
    Liem-Nguyen, Van
    et al.
    School of Science and Technology, Örebro University, Örebro, Sweden; Department of Environmental Science, Stockholm University, Stockholm, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Dinh, Ngoc Phuoc
    Diduco AB, Umeå, Sweden.
    Huy, Duong Huu
    Faculty of Food Science and Technology, Ho Chi Minh City University of Food Industry, Ho Chi Minh City, Viet Nam.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Removal mechanism of arsenic (V) by stainless steel slags obtained from scrap metal recycling2020In: Journal of Environmental Chemical Engineering, E-ISSN 2213-3437, Vol. 8, no 4, article id 103833Article in journal (Refereed)
    Abstract [en]

    With this study, the removal mechanisms of arsenate by steel slag and its potential for treatment of contaminated water were elucidated. While original slag showed a poor fit to the Langmuir equation (R2 = 0.960), washed slag (the original slag is washed by low pH water solutions to remove readily soluble oxides) conformed better (R2 = 0.995). An initial pH of 2.0 give optimal adsorption, with a strong impact from the chemical speciation observed with highest efficiency for the fully protonated (OH)3AsO form. Adsorption capacity of the slag is 4.0 mg g−1, while together with precipitation the retention capacity reaches 13.7 mg g−1. However, removal by precipitation is a non-steady process due to re-dissolution of Ca3(AsO4)2(s). The washed slag shows a similar adsorption capacity to the original one but has not as strong alkaline properties. Batch experiment shows fast adsorption kinetics and column loading tests indicate an instant adsorption kinetics with 80 % As(V) removal for a 10 mg L−1 As(V) solution by 1.0 g of washed slag using a solution flowrate of 1 mL min−1. Common ions like sulfate, carbonate, chloride, iron(III), humic acid and fulvic acid do not significantly interfere with the removal efficiency. In combination with limited hazardous metals leaching, the slag is thus appropriate for use as a filter material for treatment of contaminated water and it has been successfully applied as filter material for treatment of arsenate spiked natural water sample with average removal efficiency of 84 % (solid to liquid ratio of 200).

  • 39.
    Nilsson, Charlotte
    et al.
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Phosphorus speciation in sewage sludge and ashes from three municipal WWTPs in Sweden: investigating the possibilities for phosphorus recoveryManuscript (preprint) (Other academic)
  • 40.
    Nilsson, Charlotte
    et al.
    Örebro University, School of Science and Technology. Ekokem AB, Kumla, Sweden.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    von Kronhelm, Thomas
    Ekokem AB, Kumla, Sweden.
    Municipal sludge ash for abatement of ARD2016In: Mining Meets Water – Conflicts and Solutions: IMWA 2016 in Leipzig, Germany, July 11–15, 2016 / [ed] Drebenstedt, C. & Paul, M., Freiberg: TU Bergakademie Freiberg, Institute of Mining and Special Civil Engineering , 2016, p. 699-705Conference paper (Refereed)
    Abstract [en]

    Abatement of ARD with passive treatment systems can quite often rely only on pH-control if the iron content is high enough and to allow for the formation of ferric hydrous oxides, which act as efficient adsorbents. The stability of ferric hydrous oxides is sensitive to lowering of pH as well as Eh why they must be controlled. Hence, it would be favourable to use a well ordered adsorbent that is stable over time and under the chemical conditions of ARD. Municipal waste water sludge is a growing problem in many countries and incineration under oxidative conditions can be used to oxidize anthropogenic organic molecules which pose a threat to the environment. Sludge ashes rendering from wastewater treatment in which iron is used as a flocculation agent have high concentrations of calcium/ magnesium and ferric oxides, and should therefore, in theory be a suitable candidate for treatment of ARD. This study has therefore focused on the ability for these ashes to act as a sorbent for the removal of metals from ARD. The stability and potential release of metals from the material were quantified in batch experiments by extraction at pH 2-10, resulting in equilibrium concentrations (at pH 8) of 11.9, 0.08 and 24.1 mg L-1 for Al, Fe and Mn respectively. However, after washing with water the corresponding values were 0.01, 0.03 and 0.09 mgL(-1). In fact, after washing the sludge ash is stable from pH 4 to 10, with only slightly higher concentrations found at pH 2. Batch experiments on metal adsorption from ARD showed more than 99% sorption of Cr, Cu, Pb and V, corresponding values for Co, Ni and Zn were 56, 86 and 34% respectively. The overall results from this study show that sludge ashes are a promising solution for treatment of ARD.

  • 41.
    Nilsson, Charlotte
    et al.
    Örebro University, School of Science and Technology. Fortum Waste Solutions AB, Kumla, Sweden.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Grandin, Anna
    Örebro University, Örebro, Sweden.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Allard, B.
    Örebro University, School of Science and Technology.
    von Kronhelm, Thomas
    Fortum Waste Solutions AB, Kumla, Sweden.
    Phosphorus speciation in sewage sludge from three municipal wastewater treatment plants in Sweden and their ashes after incineration2022In: Waste Management & Research, ISSN 0734-242X, E-ISSN 1096-3669, Vol. 40, no 8, p. 1267-1276, article id 734242X211065231Article in journal (Refereed)
    Abstract [en]

    Given the high efficiency in phosphorus removal at municipal wastewater treatment plants (MMWWTP), sewage sludge constitutes a promising resource for phosphorus (P) recovery. Sewage sludge is, however, a complex matrix and its direct use as fertiliser is limited by its content of metals/metalloids and organic pollutants. In order to increase its usability as a potential resource of P, there is a need for increased knowledge on phosphorus speciation in these matrices. The sludge composition is highly influenced by local conditions (i.e. wastewater composition and treatment method), and it is therefore important to study sludge from several MMWWTPs. In this study, three different protocols for sequential extraction were utilised to investigate the chemical speciation of phosphorus in sludge from three different MMWWTP sludges in Sweden, as well as in corresponding ashes following incineration. The results showed that the total amounts of phosphorus ranged from 26 to 32 mg g-1 sludge (dry weight), of which 79-94% was inorganically bound (IP). In the sludge, 21-30% of the IP was associated with calcium (Ca-P), which is the preferred species for fertiliser production. Following incineration, this fraction increased to 54-56%, mainly due to transformation of iron-associated phosphorus (Fe-P), while aluminium-associated species of phosphorus (Al-P) remained unaltered. The results from this study confirm that incineration is a suitable treatment for sewage sludge in terms of potential phosphorus recovery.

  • 42.
    Ogar, Anna
    et al.
    Jagiellonian University, Krakow, Poland.
    Grandin, Anna
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Turnau, Katarzyna
    Jagiellonian University, Krakow, Poland.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Stabilization of Uranium(VI) at Low pH by Fungal Metabolites: Applications in Environmental Biotechnology2014In: APCBEE Procedia, ISSN 2212-6708, Vol. 10, p. 142-148Article in journal (Refereed)
    Abstract [en]

    Uranium contamination of soils and water is a worldwide problem due to geology or anthropogenic release such as mining, or use of inorganic fertilizers. In situ remediation of low and moderately contaminated sites is a complicated procedure due to the complex chemistry of uranium. This study demonstrates that at pH 3.5, a fungal strain isolated from unprocessed uranium bearing shale creates hydrochemical conditions that immobilize 97% of a total of 10 mg L-1 dissolved uranium in a 0.20 μm pore system. The redistribution occurred within 10 minutes and remained for five weeks and just 12% of the inventory was retrieved in the biomass. Size exclusion chromatography of the dissolved phase identified organic substances in the range of more than 60 kD down to 100 D as a response to time of incubation. Geochemical modeling indicates formation of uranium-organic complexes where ligand size, coordination chemistry and their tendency to agglomerate determine the redistribution.

  • 43.
    Ogar, Anna
    et al.
    Jagellonian University, Krakow, Poland.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Phytostabilization of uranium-containing shale residues using Hieracium pilosella2014In: Uranium Mining and Hydrogelogy: Uranium - Past and Future Challenges / [ed] Broder J. Merkel and Alireza Arab, Springer Publishing Company, 2014, p. 425-432Conference paper (Refereed)
    Abstract [en]

    A greenhouse pot experiment was conducted to evaluate the feasibility of using Hieracium pilosella and soil microorganisms for phytostabilization of uranium-containing shale residues. Conductivity of leachates significantly decreased and pH increased when plants were grown on the substratum. H. pilosella has ability to change the hydrochemical parameters and to decrease the mobilization of uranium. Moreover, H. pilosella is able to accumulate significant amounts of uranium in the shoots.

  • 44. Ogar, Anna
    et al.
    Sobczyk, L.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Turnau, Katarzyna
    Plant-associated microbes in heavy metal phytoremediation: a network of interaction2014In: / [ed] E. Kothe and G. Büchel, 2014, p. 6-Conference paper (Refereed)
  • 45.
    Paylar, Berkay
    et al.
    Örebro University, School of Science and Technology.
    Asnake, Solomon
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Ragnvaldsson, Daniel
    Envix Nord AB, Umeå, Sweden.
    Jass, Jana
    Örebro University, School of Science and Technology.
    Olsson, Per-Erik
    Örebro University, School of Science and Technology.
    Influence of water hardness on zinc toxicity in Daphnia magna2022In: Journal of Applied Toxicology, ISSN 0260-437X, E-ISSN 1099-1263, Vol. 42, no 9, p. 1510-1523Article in journal (Refereed)
    Abstract [en]

    Zinc is an essential trace metal required for the maintenance of multiple physiological functions. Due to this, organisms can experience both zinc deficiency and toxicity. Hardness is recognized as one of the main modifying physiochemical factors regulating zinc bioavailability. Therefore, the present study analyzed the effect of hardness on zinc toxicity using Daphnia magna. Endpoint parameters were acute-toxicity, development, reproduction, and expression data for genes involved in metal regulation and oxidative stress. In addition, the temporal expression profiles of genes during the initiation of reproduction and molting were investigated. Water hardness influenced the survival in response to exposures to zinc. A zinc concentration of 50μg/L in soft water (50 mg CaCO3 /L) caused 73% mortality after 96h exposure, whereas the same zinc concentration in the hardest water did not cause any significant mortality. Moreover, increasing water hardness from 100 to 200mg CaCO3 /L resulted in a reduced number of offspring. Fecundity was higher at first brood for groups exposed to higher Zn concentrations. The survival data was used to assess the precision of the bioavailability models (Bio-met) and the geochemical model (Visual MINTEQ). As the Bio-met risk predictions overestimated the Zn toxicity, a competition-based model to describe the effects of hardness on zinc toxicity is proposed. This approach can be used to minimize differences in setting environmental quality standards. Moreover, gene expression data showed that using the toxicogenomic approach was more sensitive than the physiological endpoints. Therefore, data presented in the study can be used to improve risk assessment for zinc toxicity.

  • 46.
    Rai, Neha
    et al.
    Örebro University, School of Science and Technology. The Life Science Centre - Biology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Forsberg, Göran
    Detectum AB, Jönköping, Sweden.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Olsson, Per-Erik
    Örebro University, School of Science and Technology. The Life Science Centre - Biology.
    Jass, Jana
    Örebro University, School of Science and Technology. The Life Science Centre - Biology.
    Metal contaminated soil leachates from an art glass factory elicit stress response, alter fatty acid metabolism and reduce lifespan in Caenorhabditis elegans2019In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 651, p. 2218-2227Article in journal (Refereed)
    Abstract [en]

    The present study evaluated the toxicity of metal contamination in soils from an art glass factory in Smaland Sweden using a Caenorhabditis elegans nematode model. The aim of the study was to chemically analyze the soil samples and study the biological effects of water-soluble leachates on the nematodes using different physiological endpoints. The total metal content showed that As, Cd and Pb were at levels above the guideline values for soils in areas around the factory. Less than 10% of the total metal content in the soil was found in the water-soluble leachates, however, Al, As, Fe and Pb remained higher than the guideline values for safe drinking water. Exposure of C. elegans to the water-soluble leachates, at both post-hatching larvae stage (L1-young adult) for 48 h and at the young adult stage (L4) for 6 h, showed significant gene alteration. Although the nematodes did not exhibit acute lethality, lifespan was significantly reduced upon exposure. C. elegans also showed altered gene expression associated with stress response and fat metabolism, as well as enhanced accumulation of body fat. The study highlighted the significance of assessing environmental samples using a combination of gene expression analysis, fatty acid metabolism and lifespan for providing valuable insight into the negative impact of metals. The altered fat metabolism and reduced lifespan on exposure to soil leachates motivates further studies to explore the mechanism of the toxicity associated with the metals present in the environment.

  • 47.
    Rist, Sinja
    et al.
    National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
    Rask, Sofie
    National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
    Ntinou, Iliana V.
    Department of Biological Sciences, University of Bergen, 5006 Bergen, Norway; Bjerknes Centre for Climate Research, University of Bergen, 5006 Bergen, Norway.
    Varpe, Øystein
    Department of Biological Sciences, University of Bergen, 5006 Bergen, Norway; Bjerknes Centre for Climate Research, University of Bergen, 5006 Bergen, Norway; Norwegian Institute for Nature Research, 5006 Bergen, Norway.
    Lindegren, Martin
    National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
    Ugwu, Kevin
    Örebro University, School of Science and Technology.
    Larsson, Maria
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Nielsen, Torkel Gissel
    National Institute of Aquatic Resources (DTU Aqua), Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
    Cumulative Impacts of Oil Pollution, Ocean Warming, and Coastal Freshening on the Feeding of Arctic Copepods2024In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851Article in journal (Refereed)
    Abstract [en]

    The Arctic is undergoing rapid changes, and biota are exposed to multiple stressors, including pollution and climate change. Still, little is known about their joint impact. Here, we investigated the cumulative impact of crude oil, warming, and freshening on the copepod species Calanus glacialis and Calanus finmarchicus. Adult females were exposed to ambient conditions (control; 0 °C + 33 psu) and combined warming and freshening: 5 °C + 27 psu (Scenario 1), 5 °C + 20 psu (Scenario 2) for 6 days. All three conditions were tested with and without dispersed crude oil. In Scenario 1, fecal pellet production (FPP) significantly increased by 40-78% and 42-122% for C. glacialis and C. finmarchicus, respectively. In Scenario 2, FPP decreased by 6-57% for C. glacialis, while it fluctuated for C. finmarchicus. For both species, oil had the strongest effect on FPP, leading to a 68-83% reduction. This overshadowed the differences between climatic scenarios. All variables (temperature, salinity, and oil) had significant single effects and several joint effects on FPP. Our results demonstrate that Arctic copepods are sensitive to environmentally realistic concentrations of crude oil and climate change. Strong reductions in feeding can reduce the copepods' energy content with potential large-scale impacts on the Arctic marine food web.

  • 48.
    Saqib, Naeem
    et al.
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Flotation tailings as a copper resource - Extraction and characterization through chemical leachingManuscript (preprint) (Other academic)
  • 49.
    Sartz, Lotta
    et al.
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    ARD treatment in sequential filter sections: efficiency of different alkaline waste materials2010In: Mine water and innovative thinking: proceedings 2010 / [ed] Christian Wolkersdorfer, Antje Freund, Nova Scotia, Canada: Cape Breton University Press , 2010, p. 271-274Conference paper (Refereed)
    Abstract [en]

    Six alkaline waste materials are tested as potential filter materials for ARD treatment. The study is an ongoing project since 2.5 years on a mine waste remediation test field in  opparberg, Sweden. The filters (0.5 m3) are operating under field conditions and general parameters (pH, electric conductivity, redox potential (Eh), alkalinity, acidity and sulphate) are measured immediately after sampling in a mobile laboratory. The reactive (alkaline) materials are followed by other filter materials in sequence with non-reactive support materials, to ensure iron/aluminum precipitation and trace metal sorption.

  • 50.
    Sartz, Lotta
    et al.
    Örebro University, School of Science and Technology.
    Bäckström, Mattias
    Örebro University, School of Science and Technology.
    Sjöberg, Viktor
    Örebro University, School of Science and Technology.
    Karlsson, Stefan
    Örebro University, School of Science and Technology.
    Successive neutralization, precipitation and trace metal immobilization in meso-scale filters for ARD treatmentManuscript (preprint) (Other academic)
    Abstract [en]

    Six alkaline by-products were studied in reactive filters (3×0.4 m3) built on the historic mine site Ljusnarsbergsfältet, Kopparberg, Sweden. Each filter was made out of three connected sections in a sequence (neutralization – oxidation (precipitation) – sorption). Total buffering capacity of the alkaline materials was found to have a minor impact on their respective performances. Other chemical and physical circumstances were more important, e.g. carbonation, iron precipitation and preferential flow path formations. A combination of fresh fly ash and lime mud had similar chemical responses as a carbonated fly ash. These filters were also the worst performers, considering overall trace element immobilizations.

    No particular differences were found between two different passive adsorbents in the oxidation stage, where iron and aluminum were intended to precipitate and act as sorbent phases for other elements: Cu, Zn, Cd, and Pb. In general it was found that highly alkaline materials containing alkalinities as CaO/Ca(OH)2 were superior to materials with carbonate alkalinity.

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  • text
  • asciidoc
  • rtf