oru.sePublikationer
Ändra sökning
Avgränsa sökresultatet
1 - 11 av 11
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Träffar per sida
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
Markera
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1.
    Holster, Savanne
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Faecal Microbiota Transfer in Irritable Bowel Syndrome and Collagenous Colitis: Clinical outcomes and host-microbe interactions2020Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Faecal microbiota transfer (FMT) aims at restoring a disturbed gut microbiotaby introducing faecal material from a healthy donor into a patient’s intestine. This approach is known as a safe and effective treatment in patients with recurrent Clostridioides difficile infection. This thesis describes the outcomes of two clinical studies in which FMT was investigated as a therapy for irritable bowel syndrome (IBS) patients andin collagenous colitis (CC) patients. Paper I showed that there were no significant differences in IBS symptom scores between patients receiving faecal material from a healthy donor (allogenic FMT) and patients receiving their own faecal material back (autologous FMT). However, unlike autologous FMT, allogenic FMT significantly decreased symptom scores compared to baseline. Additionally, allogenic FMT wasshown to alter the faecal as well as the mucosal microbiota of the IBSpatients. However, also the autologous FMT had an effect on the faecal and mucosal microbiota indicating that the bowel cleansing and/or theprocessing of the autologous faecal material affected the gut microbiota. Paper II showed that the allogenic FMT evoked a clearly different host response in IBS patients than the autologous FMT. Paper III showedthat allogenic FMT did not result in altered faecal metabolite profilesbut in disturbed interactions between the gut microbiota and its metabolites compared to autologous FMT. Paper IV describes the outcomes of the second clinical study in which repeated FMTs resulted inless diarrhoea in a subset of the treated CC patients.

    Although symptoms improved in both clinical studies, the introduction of a new microbiota by FMT did not seem to be the miracle curefor IBS and CC. However, a subset of patients could benefit from FMT, and a future challenge is to identify this subset. The findings of this thesis are essential for designing further studies aimed at increasing FMT efficacy.

    Delarbeten
    1. The Effect of Allogenic Versus Autologous Fecal Microbiota Transfer on Symptoms, Visceral Perception and Fecal and Mucosal Microbiota in Irritable Bowel Syndrome: A Randomized Controlled Study
    Öppna denna publikation i ny flik eller fönster >>The Effect of Allogenic Versus Autologous Fecal Microbiota Transfer on Symptoms, Visceral Perception and Fecal and Mucosal Microbiota in Irritable Bowel Syndrome: A Randomized Controlled Study
    Visa övriga...
    2019 (Engelska)Ingår i: Clinical and Translational Gastroenterology, ISSN 2155-384X, E-ISSN 2155-384X, Vol. 10, nr 4, artikel-id e00034Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    OBJECTIVES: Fecal microbiota transfer (FMT) is suggested as a potential treatment for patients with irritable bowel syndrome (IBS). We aimed to study the effect of allogenic and autologous FMT on IBS symptoms, visceral sensitivity, and compositional changes in fecal and mucosa-adherent microbiota.

    METHODS: Seventeen patients with IBS were randomized either to receive fecal material from a healthy donor (allogenic) or to receive their own fecal material (autologous). The fecal material was administered into the cecum by whole colonoscopy after bowel cleansing.

    RESULTS: No significant differences were found between the allogenic and the autologous FMT regarding symptom scores. However, symptom scores of patients receiving allogenic fecal material significantly decreased after FMT compared with baseline (P 5 0.02), which was not the case in the autologous group (P50.16). Visceral sensitivity was not affected except for a small beneficial effect on urge scores in the autologous group (P < 0.05). While both fecal and mucosa-adherent microbiota of some patients shifted to their respective donor’s fecal microbiota, some patients showed no relevant microbial changes after allogenic FMT. Large compositional shifts in fecal and mucosa-adherent microbiota also occurred in the autologous group.

    CONCLUSIONS: This study showed that a single FMT by colonoscopy may have beneficial effects in IBS; however, the allogenic fecal material was not superior to the autologous fecal material. This suggests that bowel cleansing prior to the colonoscopy and/or processing of the fecal material as part of the FMT routine contribute to symptoms and gut microbiota composition changes in IBS.

    Ort, förlag, år, upplaga, sidor
    Nature Publishing Group, 2019
    Nationell ämneskategori
    Gastroenterologi
    Forskningsämne
    Medicin
    Identifikatorer
    urn:nbn:se:oru:diva-74035 (URN)10.14309/ctg.0000000000000034 (DOI)000466787000001 ()31009405 (PubMedID)
    Forskningsfinansiär
    Stiftelsen Svensk Näringsforskning
    Anmärkning

    Funding Agencies:

    SIAM Gravitation Grant  024.002.002 

    Spinoza 2008 Award of the Netherlands Organization for Scientific Research (NWO) 

    Tillgänglig från: 2019-05-06 Skapad: 2019-05-06 Senast uppdaterad: 2019-12-20Bibliografiskt granskad
    2. Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome
    Öppna denna publikation i ny flik eller fönster >>Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome
    Visa övriga...
    2019 (Engelska)Ingår i: Biomolecules, E-ISSN 2218-273X, Vol. 9, nr 10, artikel-id 586Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Faecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. This study aims to investigate the colonic mucosa gene expression response to allogenic (from a donor) or autologous (own) FMT in patients with irritable bowel syndrome (IBS). In a recently conducted randomised, double-blinded, controlled clinical study, 17 IBS patients were treated with FMT by colonoscopy. RNA was isolated from colonic biopsies collected by sigmoidoscopy at baseline, as well as two weeks and eight weeks after FMT. In patients treated with allogenic FMT, predominantly immune response-related gene sets were induced, with the strongest response two weeks after the FMT. In patients treated with autologous FMT, predominantly metabolism-related gene sets were affected. Furthermore, several microbiota genera showed correlations with immune-related gene sets, with different correlations found after allogenic compared to autologous FMT. This study shows that the microbe–host response is influenced by FMT on the mucosal gene expression level, and that there are clear differences in response to allogenic compared to autologous FMT.

    Ort, förlag, år, upplaga, sidor
    MDPI, 2019
    Nyckelord
    Faecal microbiota transfer, Faecal microbiota transplantation, irritable bowel syndrome, gene expression, gut microbiota, host-microbe interaction
    Nationell ämneskategori
    Gastroenterologi
    Forskningsämne
    Medicin
    Identifikatorer
    urn:nbn:se:oru:diva-77171 (URN)10.3390/biom9100586 (DOI)000497726800078 ()31597320 (PubMedID)2-s2.0-85073107066 (Scopus ID)
    Forskningsfinansiär
    KK-stiftelsen
    Anmärkning

    Funding Agencies:

    European Society of Clinical Nutrition and Metabolism (ESPEN)  

    Netherlands Organization for Scientific Research (NWO)

    Tillgänglig från: 2019-10-10 Skapad: 2019-10-10 Senast uppdaterad: 2020-03-11Bibliografiskt granskad
    3. Faecal microbiota transfer in irritable bowel syndrome results inaltered correlations between the gut microbiota and its metabolites
    Öppna denna publikation i ny flik eller fönster >>Faecal microbiota transfer in irritable bowel syndrome results inaltered correlations between the gut microbiota and its metabolites
    Visa övriga...
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nationell ämneskategori
    Allmänmedicin
    Identifikatorer
    urn:nbn:se:oru:diva-78657 (URN)
    Tillgänglig från: 2019-12-16 Skapad: 2019-12-16 Senast uppdaterad: 2019-12-20Bibliografiskt granskad
    4. Faecal microbiota transfer in patients with microscopic colitis: A proof-of-concept study in collagenous colitis
    Öppna denna publikation i ny flik eller fönster >>Faecal microbiota transfer in patients with microscopic colitis: A proof-of-concept study in collagenous colitis
    Visa övriga...
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nationell ämneskategori
    Allmänmedicin
    Identifikatorer
    urn:nbn:se:oru:diva-78659 (URN)
    Tillgänglig från: 2019-12-16 Skapad: 2019-12-16 Senast uppdaterad: 2019-12-16Bibliografiskt granskad
  • 2.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Repsilber, Dirk
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Fecal Microbiota Transplantation in Irritable Bowel Syndrome and a Randomized Placebo-Controlled Trial2017Ingår i: 2017 DDW Abstracts, Saunders Elsevier, 2017, Vol. 152, nr 5, s. S101-S102Konferensbidrag (Övrigt vetenskapligt)
  • 3.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Hooiveld, Guido J.
    Nutrition, Metabolism and Genomics group, Division of Human Nutrition and Health, Wageningen University, Wageningen, The Netherlands.
    Repsilber, Dirk
    Örebro universitet, Institutionen för medicinska vetenskaper.
    de Vos, Willem
    Laboratory of Microbiology, Wageningen University and Research Centre and Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome2019Ingår i: Biomolecules, E-ISSN 2218-273X, Vol. 9, nr 10, artikel-id 586Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Faecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. This study aims to investigate the colonic mucosa gene expression response to allogenic (from a donor) or autologous (own) FMT in patients with irritable bowel syndrome (IBS). In a recently conducted randomised, double-blinded, controlled clinical study, 17 IBS patients were treated with FMT by colonoscopy. RNA was isolated from colonic biopsies collected by sigmoidoscopy at baseline, as well as two weeks and eight weeks after FMT. In patients treated with allogenic FMT, predominantly immune response-related gene sets were induced, with the strongest response two weeks after the FMT. In patients treated with autologous FMT, predominantly metabolism-related gene sets were affected. Furthermore, several microbiota genera showed correlations with immune-related gene sets, with different correlations found after allogenic compared to autologous FMT. This study shows that the microbe–host response is influenced by FMT on the mucosal gene expression level, and that there are clear differences in response to allogenic compared to autologous FMT.

  • 4.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Lindqvist, Carl Mårten
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Repsilber, Dirk
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Salonen, Anne
    Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
    de Vos, Willem
    Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland; Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, the Netherlands.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    The Effect of Allogenic Versus Autologous Fecal Microbiota Transfer on Symptoms, Visceral Perception and Fecal and Mucosal Microbiota in Irritable Bowel Syndrome: A Randomized Controlled Study2019Ingår i: Clinical and Translational Gastroenterology, ISSN 2155-384X, E-ISSN 2155-384X, Vol. 10, nr 4, artikel-id e00034Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    OBJECTIVES: Fecal microbiota transfer (FMT) is suggested as a potential treatment for patients with irritable bowel syndrome (IBS). We aimed to study the effect of allogenic and autologous FMT on IBS symptoms, visceral sensitivity, and compositional changes in fecal and mucosa-adherent microbiota.

    METHODS: Seventeen patients with IBS were randomized either to receive fecal material from a healthy donor (allogenic) or to receive their own fecal material (autologous). The fecal material was administered into the cecum by whole colonoscopy after bowel cleansing.

    RESULTS: No significant differences were found between the allogenic and the autologous FMT regarding symptom scores. However, symptom scores of patients receiving allogenic fecal material significantly decreased after FMT compared with baseline (P 5 0.02), which was not the case in the autologous group (P50.16). Visceral sensitivity was not affected except for a small beneficial effect on urge scores in the autologous group (P < 0.05). While both fecal and mucosa-adherent microbiota of some patients shifted to their respective donor’s fecal microbiota, some patients showed no relevant microbial changes after allogenic FMT. Large compositional shifts in fecal and mucosa-adherent microbiota also occurred in the autologous group.

    CONCLUSIONS: This study showed that a single FMT by colonoscopy may have beneficial effects in IBS; however, the allogenic fecal material was not superior to the autologous fecal material. This suggests that bowel cleansing prior to the colonoscopy and/or processing of the fecal material as part of the FMT routine contribute to symptoms and gut microbiota composition changes in IBS.

  • 5.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Repsilber, Dirk
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Faecal microbiota transfer in irritable bowel syndrome: clinical outcomes of a randomised placebo-controlled trial2017Ingår i: UEG Week 2017 Oral Presentations, Sage Publications, 2017, Vol. 5, s. A155-A156Konferensbidrag (Refereegranskat)
  • 6.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Repsilber, Dirk
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Geng, Dawei
    Science and Engineering, School of Science and Technology, Örebro University, Sweden.
    Hyötyläinen, Tuulia
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Lindqvist, Carl Mårten
    Örebro universitet, Institutionen för medicinska vetenskaper.
    de Vos, W.
    Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland; Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Faecal microbiota transfer in irritable bowel syndrome results inaltered correlations between the gut microbiota and its metabolitesManuskript (preprint) (Övrigt vetenskapligt)
  • 7.
    Holster, Savanne
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Rode, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Bohr, Johan
    Örebro universitet, Institutionen för medicinska vetenskaper. Region Örebro län. Department of Gastroenterology.
    Kumawat, Ashok Kumar
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Hultgren Hörnquist, Elisabeth
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Faecal microbiota transfer in patients with microscopic colitis: A proof-of-concept study in collagenous colitisManuskript (preprint) (Övrigt vetenskapligt)
  • 8.
    König, Julia
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Holster, Savanne
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Bruins, Maaike
    DSM Biotechnology Centre, Delft, Netherlands.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Aspergillus Niger-Derived Enzyme Degrades Gluten in the Stomach of Gluten-Sensitive Subjects2017Ingår i: 2017 DDW Abstracts, Saunders Elsevier, 2017, Vol. 152, nr 5, s. S481-S481Konferensbidrag (Övrigt vetenskapligt)
  • 9.
    König, Julia
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Holster, Savanne
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Bruins, Maaike
    DSM Biotechnology Centre, Delft, Netherlands.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Effective gluten degradation in non-coeliac gluten-sensitive subjects by Aspergillus Niger derived enzyme: A placebo-controlled randomized clinical trial2017Konferensbidrag (Refereegranskat)
  • 10.
    König, Julia
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Holster, Savanne
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Maaike, Bruins
    DSM Biotechnology Centre, Delft, Netherlands.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Randomized clinical trial: Effective gluten degradation by Aspergillus niger-derived enzyme in a complex meal setting2017Ingår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, nr 13100Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The Aspergillus niger-derived prolyl endoprotease (AN-PEP) has previously been shown to degrade gluten in healthy subjects when added to an intragastrically infused meal. The current study investigated the efficacy of AN-PEP in a physiological meal setting. In this randomized placebo-controlled crossover study, 18 gluten-sensitive subjects consumed a porridge containing 0.5 g gluten together with two tablets either containing a high or low dose of AN-PEP, or placebo. Gastric and duodenal content was sampled over 180 minutes, and areas under the curve of gluten concentrations were calculated. The primary outcome, i.e. success rate of high dose AN-PEP defined as at least 50% gluten degradation compared to placebo in the duodenum, was achieved in 10 of 13 comparisons. In the stomach, gluten levels were reduced from 176.9 (median, interquartile range 73.5–357.8) to 22.0 (10.6–50.8, p = 0.001) in the high dose and to 25.4 μg × min/ml (16.4–43.7, p = 0.001) in the low dose. In the duodenum, gluten levels were reduced from 14.1 (8.3–124.7) in the placebo to 6.3 (3.5–19.8, p = 0.019) in the high dose and to 7.4 μg × min/ml in the low dose (3.8–12.0, p = 0.015). Thus even in a physiological meal setting, AN-PEP significantly degraded most gluten in the stomach before it entered the duodenum.

  • 11.
    Marques, Tatiana M.
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Holster, Savanne
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Wall, Rebecca
    Örebro universitet, Institutionen för medicinska vetenskaper.
    König, Julia
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Correlating the gut microbiome to health and disease2016Ingår i: The Gut-Brain Axis: Dietary, Probiotic, and Prebiotic Interventions on the Microbiota / [ed] Niall Hyland, Catherine Stanton, Elsevier, 2016, s. 261-291Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [en]

    The gut microbiota is a complex ecosystem consisting of a diverse population of prokaryotes that has a symbiotic relationship with its host; thus it plays a vital role for the host’s health. Our understanding of the effect of the gut microbiome in health and disease has grown substantially over the past 2 decades, mostly because of recent advances in sequencing and other high-throughput technologies. Given its high metabolic potential, close proximity to the intestinal mucosa, and interaction with the immune system, it is not surprising that the gut microbiome is an important partaker in human health. Evidence to the importance of the gut microbiome in human health and disease is the growing number of conditions now linked to changes in the resident gut microbiota, including recurrent Clostridium difficile infections, inflammatory bowel disease, irritable bowel syndrome, colorectal cancer, allergies, neurological diseases, and metabolic diseases. Research into this field of the association of the gut microbiome with health and disease continues to expand at a rapid pace as we come to accept the gut microbiome as our “second genome.” Targeting the gut microbiome to restore/modulate its composition with the use of antibiotics, probiotics, prebiotics, and even fecal microbiota transplantation is considered a promising future strategy for the development of new solutions in the treatment of various diseases associated with an imbalance in microbiota composition and functioning.

1 - 11 av 11
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf