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  • 101.
    Meerits, Jonna
    Örebro University, School of Health and Medical Sciences.
    Blodtrycksmanschett eller manuell handkompression vid bedömning av venös insufficiens i Vena saphena magna2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    Bakgrund: Venduplex har på senare år vuxit fram som den mest använda metoden vid diagnostik av venös insufficiens eftersom det är en ofarlig, kostnadseffektiv, non-invasiv metod med hög sensitivitet och specificitet där både den anatomiska placeringen av insufficiensen samt den funktionella förändringen av venen kan påvisas direkt vid undersökningen. Olika manövrar kan genomföras för att påfresta venklaffarna och därmed framkalla en eventuell reflux, men det är oklart om de olika manövrerna kan påverka den reflux som provoceras fram och därmed också påverkar bedömningen av insufficiensgraden.

    Syfte: Syftet med studien var att undersöka om det förelåg skillnad i graderingen av insufficiensen samt om den maximala refluxhastigheten påverkades beroende på vilken manöver som användes för att framkalla en reflux.

    Metod: 20 v. saphena magna mitt på låret med påvisad reflux deltog i studien. Två upprepade mätningar genomfördes där venklaffarna provocerades med hjälp av en blodtrycksmanschett över vaden, med en manuell kompression över låret samt med en manuell kompression över vaden.

    Resultat: Den maximala refluxhastigheten blev 0,33±0,20m/s när en blodtrycksmanschett över vaden användes, 0,31±0,22m/s när en manuell handkompression över vaden användes samt 0,17±0,16m/s när en manuell lårkompression genomfördes.

    Slutsats: Ingen signifikant skillnad på maximal refluxhastighet kunde konstateras när en automatisk uppblåsbar blodtrycksmanschett över vaden jämfördes med en manuell handkompression över vaden. En signifikant högre maximal refluxhastighet konstaterades när en manuell vadkompression jämfördes med en manuell lårkompression.

  • 102.
    Mezan, Daniela
    Örebro University, School of Health Sciences.
    Manuell och automatisk analys av apné-hypopné index (AHI) under sömn vid frågeställning obstruktiv sömnapné2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 103.
    Mirvic, Sejla
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Betydelsen av polymorfismen rs4353135 i NLRP3 genen för utveckling av kardiovaskulära sjukdomar2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 104.
    Mogren, Sofia
    Örebro University, School of Health Sciences.
    Evaluation of mast cell tryptase in wound healing response in the bronchial epithelium2019Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Background: The inflammatory response in asthma is a complex interaction between structural cells and inflammatory cells. Increased mast cell (MC) densities in the airway epithelium is a hallmark of asthma. However, little is known regarding the role and the effect of MC mediators on epithelium, especially when damaged by respiratory viruses.

    Aim: To evaluate the effect of the MC mediator tryptase on wound healing responses on bronchial epithelial cells. Also, the effect of combined doses of tryptase and/or the viral mimic TLR3 agonist poly (I:C) is going to be investigated.

    Methods: In BEAS-2B cells the effect of tryptase and TLR3 agonist poly I:C on wound healing was studied using live cell imaging. RNA was collected and analysed with RT2 profiler PCR array for inflammation and wound healing related pathways and supernatant was collected and analysed for extra cellular protein secretion with ELISA and Luminex.

    Results: Stimulation of BEAS-2B with tryptase promoted wound healing compared to untreated controls (scratch gap closure: p<0.005). MC tryptase upregulated gene expression of epidermal growth factor ligand amphiregulin (p=0.004) and a combination of poly I:C and tryptase increased CXCL1 (p=0.002), CXCL11 (p=0.03), IL-6 (0.002) and CXCL2 (p=0.003) compared to untreated controls.

  • 105.
    Mohammed Salah, Muna
    Örebro University, School of Health Sciences.
    Intima media tjockleken hos personer med olika body mass index2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 106. Mohlin, Camilla
    et al.
    Liljekvist-Soltic, Ingela
    Johansson, Kjell
    Örebro University, School of Health and Medical Sciences.
    Further assessment of neuropathology in retinal explants and neuroprotection by human neural progenitor cells2011In: Journal of Neural Engineering, ISSN 1741-2560, E-ISSN 1741-2552, Vol. 8, no 6, p. 066012-Article in journal (Refereed)
    Abstract [en]

    Explanted rat retinas show progressive photoreceptor degeneration that appears to be caspase-12-dependent. Decrease in photoreceptor density eventually affects the inner retina, particularly in the bipolar cell population. Explantation and the induced photoreceptor degeneration are accompanied by activation of Muller and microglia cells. The goal of this study was to determine whether the presence of a feeder layer of human neural progenitor cells (hNPCs) could suppress the degenerative and reactive changes in the explants. Immunohistochemical analyses showed considerable sprouting of rod photoreceptor axon terminals into the inner retina and reduced densities of cone and rod bipolar cells. Both sprouting and bipolar cell degenerations were significantly lower in retinas cultured with feeder layer cells compared to cultured controls. A tendency toward reduced microglia activation in the retinal layers was also noted in the presence of feeder layer cells. These results indicate that hNPCs or factors produced by them can limit the loss of photoreceptors and secondary injuries in the inner retina. The latter may be a consequence of disrupted synaptic arrangement.

  • 107.
    Mordenfeld, Arne
    et al.
    Dept Oral & Maxillofacial Surg, Publ Hlth Serv, Gävle, Sweden; Ctr Res & Dev, Uppsala Univ, Gavleborg, Sweden; Dept Biomat, Inst Clin Sci, Univ Gothenburg, Gothenburg, Sweden.
    Hallman, Mats
    Dept Oral & Maxillofacial Surg, Publ Hlth Serv, Gävle, Sweden; Ctr Res & Dev, Uppsala Univ, Gävleborg, Sweden.
    Johansson, Carina B.
    Örebro University, School of Health and Medical Sciences.
    Albrektsson, Tomas
    Dept Biomat, Inst Clin Sci, Univ Gothenburg, Gothenburg, Sweden.
    Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone2010In: Clinical Oral Implants Research, ISSN 0905-7161, E-ISSN 1600-0501, Vol. 21, no 9, p. 961-970Article in journal (Refereed)
    Abstract [en]

    Objective The purpose of the present study was to histologically and histomorphometrically evaluate the long-term tissue response to deproteinized bovine bone (DPBB) particles used in association with autogenous bone and to compare particle size after 6 months and 11 years, in the same patients, in order to determine possible resorption. Material and methods Twenty consecutive patients (14 women and six men) with a mean age of 62 years (range 48-69 years) with severe atrophy of the posterior maxilla were included in this study. Thirty maxillary sinuses with < 5 mm subantral alveolar bone were augmented with a mixture of 80% DPBB and 20% autogenous bone. Eleven years (mean 11.5 years) after augmentation, biopsies were taken from the grafted areas of the 11 patients who volunteered to participate in this new surgical intervention. The following histomorphometrical measurements were performed in these specimens: total bone area in percentage, total area of the DPBB, total area of marrow space, the degree of DPBB-bone contact (percentage of the total surface length for each particle), the length of all DPBB particles and the area of all DPBB particles. The length and the area of the particles were compared with samples harvested from the same patients at 6 months (nine samples) and pristine particles from the manufacturer. Results The biopsies consisted of 44.7 +/- 16.9% lamellar bone, 38 +/- 16.9% marrow space and 17.3 +/- 13.2% DPBB. The degree of DPBB to bone contact was 61.5 +/- 34%. There were no statistically significant differences between the length and area of the particles after 11 years compared with those measured after 6 months in the same patients or to pristine particles from the manufacturer. Conclusion DPBB particles were found to be well integrated in lamellar bone, after sinus floor augmentation in humans, showing no significant changes in particle size after 11 years.

  • 108.
    Mulugeta Melkie, Zegeye
    Örebro University, School of Health Sciences.
    Impact of IL-17 cytokines on the proinflammatory responses in cultured human monocytes and vascular cells2016Independent thesis Advanced level (degree of Master (Two Years)), 10 credits / 15 HE creditsStudent thesis
  • 109.
    Naderfard, Anna
    Örebro University, School of Health and Medical Sciences.
    Extrahering av mikroRNA från plasma för användning som potentiell biomarkör2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 110.
    Nikolajeva, Jelena
    Örebro University, School of Health Sciences.
    Jämförelse av två multiplexa meningit-paneler för analys av cerebrospinalvätska från Nepal2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 111.
    Norberg, Marie-Louise
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Hållbarhetsstudie av provtagningsrör vid bestämning av SR med Excyte-20e system2012Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 112.
    Nordén, Marcus
    et al.
    Örebro University, School of Science and Technology.
    Westman, Ola
    Örebro University, School of Science and Technology.
    Venizelos, Nikolaos
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Engwall, Magnus
    Örebro University, School of Science and Technology.
    Perfluorooctane sulfonate increases β-oxidation of palmitic acid in chicken liver2012In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 19, no 5, p. 1859-1863Article in journal (Refereed)
    Abstract [en]

    Purpose: Perfluorooctane sulfonate (PFOS) belongs to a group of chemicals called perfluoroalkyl acids that have been extensively used in various applications such as stain and oil resistant treatments for fabrics, fire-fighting foams, and insecticides. These chemicals present an environmental and health risk being present in many samples both in wildlife and humans. In this study, we investigate the effect of PFOS on fatty acid β-oxidation in developing chicken embryos.

    Methods: Fertilized chicken eggs were exposed in ovo to PFOS at day 4 of incubation. On day 10, the eggs were dissected and livers were incubated in vitro with (3)H-palmitic acid for 2 h. The media were collected, and after clean up, the amount of tritiated water was measured with liquid scintillation counting to determine the rate of palmitic acid β-oxidation.

    Results: PFOS was found to induce fatty acid β-oxidation at doses starting from a lowest observed effect level (LOEL) of 0.1 μg/g egg weight. Maximum induction of 77 % compared to control was seen at 0.3 μg/g.

    Conclusions: The administered doses in which effects are seen are around and even lower than the levels that can be found in wild populations of birds. General population human levels are a factor of two to three times lower than the LOEL value of this study. The environmental contamination of PFOS therefore presents a possibility of effects in wild populations of birds.

  • 113.
    Norin, Johanna
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    A retrospective evaluation study of diagnostic accuracy of Xpert® MTB/RIF assay, used for detection of Mycobacterium tuberculosis in Greece2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 114.
    Nsona, Sandra
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Intracellulärt Ca²+ hos trombocyter efter stimulering med Porphyromonas gingivalis stammar: (betydelsen av P2Y 1-receptorer och PLC-signalvägen)2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 115.
    Olsson, Linnea
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Detection of synergistic activity of antibiotics in Klebsiella pneumoniae using MALDI-TOF MS2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 116.
    Olsson, Lovisa A.
    et al.
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Örebro University Hospital. Department of Laboratory Medicine/Clinical Chemistry, Örebro University Hospital, Örebro, Sweden.
    Hagnelius, Nils-Olof
    Örebro University Hospital. Department of Geriatrics, Örebro University Hospital, Örebro, Sweden.
    Nilsson, Torbjörn K.
    Department of Medical Biosciences, Clinical Chemistry, Umeå University, Umeå, Sweden.
    Renal function is a determinant of subjective well-being in active seniors but not in patients with subjective memory complaints2014In: BMC Research Notes, ISSN 1756-0500, E-ISSN 1756-0500, Vol. 7, no 1, article id 647Article in journal (Refereed)
    Abstract [en]

    Results: There were no significant differences in cystatin C and eGFR values between the two cohorts: cystatin C medians 0.88 vs 0.86 mg/L and eGFR 73 vs 80 mL/min/1.73 m2(AS vs DGM). In the AS cohort cystatin C was negatively related to PGWB index in women (P &lt; 0.001, R 2≈ 5%), and the covariates age and BMI did not improve the models. The renal biomarkers were unrelated to the PGWB index in the DGM cohort. Cystatin C in the AS cohort was adversely related to the PGWB subdimensions anxiety, depressed mood, positive well-being, and vitality in women, but in men only to depressed mood (P &lt; 0.006; R 2≈ 6%). In the DGM cohort, depressed mood in men was also significantly related to cystatin C (P = 0.050), but not in women.

    Background: During our whole life span, factors influencing health and functioning are accumulated. In chronic kidney disease, quality of life is adversely affected. We hypothesized that biomarkers of renal function could also be determinants of subjective well-being (SWB) in Swedish elderly subjects. SWB was assessed by the Psychological General Well-Being index (PGWB index) in two study groups: Active seniors (AS) consisted of community-dwelling elderly Swedes leading an active life (n = 389), and the DGM cohort (n = 300) consisted of subjects referred to the Memory Unit at the Department of Geriatrics for memory problems, Serum creatinine, cystatin C, and eGFR (CKD-EPI) were used as biomarkers of renal function.

    Conclusions: Renal function even within the normal range, measured by serum cystatin C concentration, has significant and sex specific associations with subjective well-being and its subdimensions in healthy elderly subjects. Maintenance of good renal function in aging may be of importance in maintaining a high subjective well-being.

  • 117.
    Onate Öberg, Bernard
    Örebro University, School of Health Sciences.
    Jämförelse av bildkvalité mellan standardsskelettscintigrafi och programmet Evolution for Bone TM i GE-healthcare gammakamera2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 118.
    Oresic, Matej
    Örebro University, School of Medical Sciences. VTT Technical Research Centre of Finland, Espoo, Finland.
    Informatics and computational strategies for the study of lipids2011In: Biochimica et Biophysica Acta, ISSN 0006-3002, E-ISSN 1878-2434, Vol. 1811, no 11, p. 991-999Article in journal (Refereed)
    Abstract [en]

    The ability to translate vast amounts of information, as obtained from lipidomic analysis, into the knowledge and understanding of biological phenomena is an important challenge faced by the lipidomics community. While many of the informatics and computational tools from other domains such as bioinformatics and metabolomics are also applicable to lipidomics data processing and analysis, new solutions and strategies are needed for the studies of lipidomes at the systems level. This is due to enormous functional and structural diversity of lipids as well as because of their complex regulation at multiple spatial and temporal scales. In order to better understand the lipidomes at the physiological level, lipids need to be modeled not only at the level of biological pathways but also at the level of the biophysical systems they are part of, such as cellular membranes or lipoprotein particles. Herein the current state, recent advances and new opportunities in the field of lipid bioinformatics are reviewed.

  • 119.
    Oresic, Matej
    et al.
    Örebro University, School of Medical Sciences. VTT Technical Research Centre of Finland, Espoo, Finland.
    Lötjönen, Jyrki
    VTT Technical Research Centre of Finland, Tampere, Finland.
    Soininen, Hilkka
    Kuopio University Hospital, University of Eastern Finland, Kuopio, Finland.
    Systems medicine and the integration of bioinformatic tools for the diagnosis of Alzheimer's disease2010In: Genome Medicine, ISSN 1756-994X, E-ISSN 1756-994X, Vol. 2, no 11, article id 83Article in journal (Refereed)
    Abstract [en]

    Because of the changes in demographic structure, the prevalence of Alzheimer's disease is expected to rise dramatically over the next decades. The progression of this degenerative and terminal disease is gradual, with the subclinical stage of illness believed to span several decades. Despite this, no therapy to prevent or cure Alzheimer's disease is currently available. Early disease detection is still important for delaying the onset of the disease with pharmacological treatment and/or lifestyle changes, assessing the efficacy of potential therapeutic agents, or monitoring disease progression more closely using medical imaging. Sensitive cerebrospinal-fluid-derived marker candidates exist, but given the invasiveness of sample collection their use in routine diagnostics may be limited. The pathogenesis of Alzheimer's disease is complex and poorly understood. There is thus a strong case for integrating information across multiple physiological levels, from molecular profiling (metabolomics, lipidomics, proteomics and transcriptomics) and brain imaging to cognitive assessments. To facilitate the integration of heterogeneous data, such as molecular and image data, sophisticated statistical approaches are needed to segment the image data and study their dependencies on molecular changes in the same individuals. Molecular profiling, combined with biophysical modeling of molecular assemblies associated with the disease, offer an opportunity to link the molecular pathway changes with cell- and tissue-level physiology and structure. Given that data acquired at different levels can carry complementary information about early Alzheimer's disease pathology, it is expected that their integration will improve early detection as well as our understanding of the disease.

  • 120.
    Palm, Eleonor
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Inflammatory responses of gingival fibroblasts in the interaction with the periodontal pathogen Porphyromonas gingivalis2015Doctoral thesis, comprehensive summary (Other academic)
    List of papers
    1. Porphyromonas gingivalis downregulates the immune response of fibroblasts
    Open this publication in new window or tab >>Porphyromonas gingivalis downregulates the immune response of fibroblasts
    2013 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 13, p. 155-Article in journal (Refereed) Published
    Abstract [en]

    Background: Porphyromonas gingivalis is a key pathogen in periodontitis, an inflammatory disease leading to destruction of bone and tooth-supporting tissue. P. gingivalis possesses a number of pathogenic properties to enhance growth and survival, including proteolytic gingipains. Accumulating data shows that gingipains are involved in the regulation of host inflammatory responses. The aim of this study was to determine if P. gingivalis infection modulates the inflammatory response of fibroblasts, including the release of chemokines and cytokines. Human gingival fibroblasts or primary dermal fibroblasts were pre-stimulated with tumor-necrosis factor-alpha (TNF-alpha) and cocultured with P. gingivalis. Gingipain inhibitors were used to explore the effect of gingipains. CXCL8 levels were determined with ELISA and the relative levels of various inflammatory mediators were determined by a cytokine assay.

    Results: TNF-alpha-triggered CXCL8 levels were completely abolished by viable P. gingivalis, whereas heat-killed P. gingivalis did not suppress CXCL8. Accumulation of CXCL8 was partially restored by an arginine-gingipain inhibitor. Furthermore, fibroblasts produced several inflammatory mediators, notably chemokines, all of which were suppressed by viable P. gingivalis.

    Conclusion: These findings provide evidence that fibroblast-derived inflammatory signals are modulated by heat-instable gingipains, whereby the bacteria can escape killing by the host immune system and promote its own growth and establishment. In addition, we show that fibroblasts are important mediators of inflammation in response to infection and thereby play a crucial role in determining the nature and magnitude of the invasion of immune cells.

    Keywords
    Porphyromonas gingivalis, Fibroblasts, Chemokines, Cytokines
    National Category
    Microbiology in the medical area
    Research subject
    Medicine
    Identifiers
    urn:nbn:se:oru:diva-30310 (URN)10.1186/1471-2180-13-155 (DOI)000322041700001 ()2-s2.0-84880027349 (Scopus ID)
    Funder
    Swedish Research CouncilSwedish Heart Lung Foundation
    Note

    Funding Agency: Foundation of Olle Engkvist; Mats Kleberg Foundation

    Available from: 2013-08-23 Created: 2013-08-23 Last updated: 2018-01-11Bibliographically approved
    2. Suppression of inflammatory responses of human gingival fibroblasts by gingipains from Porphyromonas gingivalis
    Open this publication in new window or tab >>Suppression of inflammatory responses of human gingival fibroblasts by gingipains from Porphyromonas gingivalis
    2015 (English)In: Molecular Oral Microbiology, ISSN 2041-1006, E-ISSN 2041-1014, Vol. 30, no 1, p. 74-85Article in journal (Refereed) Published
    Abstract [en]

    The interaction between human gingival fibroblasts (HGFs) and Porphyromonas gingivalis plays an important role in the development and progression of periodontitis. Porphyromonas gingivalis possesses several virulence factors, including cysteine proteases, the arginine-specific (Rgp) and lysine-specific (Kgp) gingipains. Studying the mechanisms that P.gingivalis, and its derived virulence, use to propagate and interact with host cells will increase the understanding of the development and progression of periodontitis. In this study, we aimed to elucidate how P.gingivalis influences the inflammatory events in HGFs regarding transforming growth factor-(1) (TGF-(1)), CXCL8, secretory leucocyte protease inhibitor (SLPI), c-Jun and indoleamine 2,3-dioxygenase (IDO). HGFs were inoculated for 6 and 24h with the wild-type strains ATCC 33277 and W50, two gingipain-mutants of W50 and heat-killed ATCC 33277. The P.gingivalis regulated CXCL8 and TGF-(1) in HGFs, and the kgp mutant gave significantly higher immune response with increased CXCL8 (P<0.001) and low levels of TGF-(1). We show that HGFs express and secrete SLPI, which was significantly suppressed by P.gingivalis (P<0.05). This suggests that by antagonizing SLPI, P.gingivalis contributes to the tissue destruction associated with periodontitis. Furthermore, we found that P.gingivalis inhibits the expression of the antimicrobial IDO, as well as upregulating c-Jun (P<0.05). In conclusion, P.gingivalis both triggers and suppresses the immune response in HGFs. Consequently, we suggest that the pathogenic effects of P.gingivalis, and especially the activity of the gingipains on the inflammatory and immune response of HGFs, are crucial in periodontitis.

    Keywords
    CXCL8, gingipain, indoleamine 2, 3-dioxygenase, periodontitis, secretory leucocyte protease inhibitor, transforming growth factor-β
    National Category
    Microbiology in the medical area Medical and Health Sciences
    Research subject
    Microbiology; Medicine
    Identifiers
    urn:nbn:se:oru:diva-42617 (URN)10.1111/omi.12073 (DOI)000347897100007 ()25055828 (PubMedID)2-s2.0-84920913980 (Scopus ID)
    Funder
    Swedish Research CouncilSwedish Heart Lung Foundation
    Note

    Funding Agencies:

    Foundation of Olle Engkvist

    Knowledge Foundation

    Available from: 2015-02-13 Created: 2015-02-13 Last updated: 2018-01-11Bibliographically approved
    3. The role of toll-like and protease-activated receptors in the expression of cytokines by gingival fibroblasts stimulated with the periodontal pathogen Porphyromonas gingivalis
    Open this publication in new window or tab >>The role of toll-like and protease-activated receptors in the expression of cytokines by gingival fibroblasts stimulated with the periodontal pathogen Porphyromonas gingivalis
    (English)Manuscript (preprint) (Other academic)
    National Category
    Microbiology in the medical area
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-43307 (URN)
    Available from: 2015-03-04 Created: 2015-03-04 Last updated: 2018-01-11Bibliographically approved
    4. The role of toll-like and protease-activated receptors and associated intracellular signalling in Porphyromonas gingivalis-infected gingival fibroblasts
    Open this publication in new window or tab >>The role of toll-like and protease-activated receptors and associated intracellular signalling in Porphyromonas gingivalis-infected gingival fibroblasts
    (English)Manuscript (preprint) (Other academic)
    National Category
    Microbiology in the medical area
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-43309 (URN)
    Available from: 2015-03-04 Created: 2015-03-04 Last updated: 2018-01-11Bibliographically approved
  • 121.
    Palmbrandt, Linda
    Örebro University, School of Health Sciences.
    Temperaturens inverkan på sensoriska delen av nervus medianus förmåga att leda elektriska signaler2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 122.
    Persson, Julia
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Validering av en multiplex realtids-PCR för direkt detektion av Herpes simplex virus och Varicella zoster virus2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 123.
    Pettersson, Fredrika
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Identifiering och kvantifiering av humant papillomvirus typ 16 med droplet digital PCR2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 124.
    Pinsanor, Preeyanun
    Örebro University, School of Health Sciences.
    Insamling av referensvärden för Visual evoked potentials (VEP) hos friska individer vid Centralsjukhuset i Karlstad2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 125.
    Ranta, Isabella
    Örebro University, School of Health Sciences.
    Kvantitativt sensoriskt test - tröskelvärden vid olika hudtemperaturer2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 126.
    Rask, Maja
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Jämförelse av bakterieväxt på fasta, generella anaerobodlingsmedier av fem olika fabrikat2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 127.
    Rolf, Maria
    Örebro University, School of Health and Medical Sciences.
    Utprovning av Western blot mätning av signalproteiner vid apotops av myeloida leuktocyter2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 128.
    Rosendahl, Maja
    Örebro University, School of Health Sciences.
    Validering av två varianter av Kinds reagens2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 129.
    Rumyantseva, Tatiana
    et al.
    Central Research Institute for Epidemiology, Moscow, Russia.
    Shipitsyna, Elena
    Laboratory of Microbiology, D.O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, St. Petersburg, Russia; Department of Laboratory Medicine, Microbiology, WHO Collaborating Centre for Gonorrhoea and Other STIs, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Guschin, Alexander
    Central Research Institute for Epidemiology, Moscow, Russia.
    Unemo, Magnus
    Örebro University, School of Health Sciences. Department of Laboratory Medicine, Microbiology, WHO Collaborating Centre for Gonorrhoea and Other STIs, Örebro University Hospital, Örebro, Sweden.
    Evaluation and subsequent optimizations of the quantitative AmpliSens Florocenosis/Bacterial vaginosis-FRT multiplex real-time PCR assay for diagnosis of bacterial vaginosis2016In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 12, p. 1099-1108Article in journal (Refereed)
    Abstract [en]

    Traditional microscopy-based methods for diagnosis of bacterial vaginosis (BV) are underutilized in many settings, and molecular techniques may provide opportunities for rapid, objective, and accurate BV diagnosis. This study evaluated the quantitative AmpliSens Florocenosis/Bacterial vaginosis-FRT multiplex real-time PCR (Florocenosis-BV) assay. Vaginal samples from a previous study including unselected female subjects (n = 163) and using Amsel criteria and 454 pyrosequencing for BV diagnosis were examined with the Florocenosis-BV test and additionally tested for the presence and quantity of Gardnerella vaginalis clades 3 and 4. The Florocenosis-BV assay demonstrated 100% and 98% sensitivity compared with the Amsel criteria and 454 pyrosequencing, respectively, with 91% specificity. The modified Florocenosis-BV assay (detecting also G. vaginalis clades 3 and 4) resulted in 100% sensitivity vs the Amsel criteria and 454 pyrosequencing with specificity of 86% and 88%, respectively. Further optimizations of thresholds for the quantitative parameters used in the kit resulted in 99-100% accuracy vs Amsel criteria and 454 pyrosequencing for selected parameters. The Florocenosis-BV assay is an objective, accurate, sensitive, and specific method for BV diagnosis; however, the performance of the test can be further improved with some minor optimizations.

  • 130.
    Rus Ciuca, Mihaela
    Örebro University, School of Health Sciences.
    Jämförelse av immunhistokemisk färgning för antikropparna P16, ER samt cytokeratin på cytospinglas med instrumentet Dako Autostainer Link 48 och ThinPrepglas med instrumentet Dako Omnis2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 131.
    Saad Muse, Safia
    Örebro University, School of Health Sciences.
    En jämförelsestudie av multiple sleep latency test undersökningsresultat; vid misstänkt narkolepsi mellan åren 2007-20172018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 132.
    Sahdo, Berolla
    et al.
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    DeLeo, Frank R.
    Rocky Mountins Laboratories, National Institute an Infectious Deseases, National Institute of Health, 903 South 4th St, Hamilton, Mt 59840 USA.
    Söderqvist, Bo
    Örebro University, School of Medicine, Örebro University, Sweden.
    Särndahl, Eva
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Staphylococcus aureus-mediated caspase-1 activation in human neutrophils: a role for Panton-Valentine Leukocidin?Manuscript (preprint) (Other academic)
  • 133.
    Sahdo, Berolla
    et al.
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Clinical Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Särndahl, Eva
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Clinical Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Elgh, Fredrik
    Department of Clinical Microbiology, Virology, Umeå University, Umeå, Sweden.
    Söderquist, Bo
    Örebro University, School of Medicine, Örebro University, Sweden. Örebro University Hospital.
    Propionibacterium acnes activates caspase-1 in human neutrophils2013In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 121, no 7, p. 652-63Article in journal (Refereed)
    Abstract [en]

    Propionibacterium acnes is a Gram-positive, slow-growing, anaerobic bacillus, predominantly found as a commensal on the skin and mucous membranes of adults. It is, however, also considered an opportunistic pathogen; mostly associated with acne vulgaris, but rarely also with severe infections such as infective endocarditis, prosthetic joint infections, and deep sternal wound infections following cardiothoracic surgery. In addition, P. acnes has recently been found in high frequency in prostate tissue from patients with prostatitis and prostate cancer. The NOD-like receptors (NLR) act as intracellular sensors of microbial components, and a number of various bacteria have been found to induce assembling and activation of NLR-inflammasomes; leading to a pro-inflammatory response. The inflammasome-mediated formation of the pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-18 involves the auto-proteolytic maturation of caspase-1. This study investigated if P. acnes activates inflammasomes. Propionibacterium acnes isolates (n = 29) with diverse origin were used as stimuli for peripheral leukocytes obtained from blood donors (BDs). The activity of inflammasomes was determined by measuring caspase-1 by flow cytometry and cytokine production by ELISA. A significant amount of caspase-1 was found in neutrophils upon P. acnes stimulation, whereas only a modest activation was seen in monocytes. The activation was mainly produced by components of the bacterial cell and no exo-products, because heat-killed and live bacteria caused high activation of caspase-1 as well as cytokine production, whereas the bacterial supernatant elicited minor effect. The response among different BDs varied significantly, almost fivefold. In addition, P. acnes of various origins showed considerable variation, however, the commensal isolates showed a stronger response compared with the invasive. In conclusion, although regarded as a harmless commensal of the skin, P. acnes strongly activates the inflammasome of human peripheral neutrophils.

  • 134.
    Salek, Reza M
    et al.
    European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus Hinxton, Cambridge, United Kingdom; Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
    Neumann, Steffen
    Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
    Schober, Daniel
    Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
    Hummel, Jan
    Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
    Billiau, Kenny
    Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
    Kopka, Joachim
    Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
    Correa, Elon
    School of Chemistry & Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom.
    Reijmers, Theo
    Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University, Leiden, Netherlands.
    Rosato, Antonio
    Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino FI, Italy.
    Tenori, Leonardo
    Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino FI, Italy; FiorGen Foundation, Sesto Fiorentin FI, Italy.
    Turano, Paola
    Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino FI, Italy.
    Marin, Silvia
    Department of Biochemistry and Molecular Biology, IBUB, Universitat de Barcelona, Barcelona, Spain.
    Deborde, Catherine
    INRA, Univ. Bordeaux, UMR1332 Fruit Biology and Pathology, Metabolome Facility of Bordeaux (MetaboHUB), Functional Genomics Center, IBVM, Centre INRA Bordeaux, Villenave d’Ornon, France.
    Jacob, Daniel
    INRA, Univ. Bordeaux, UMR1332 Fruit Biology and Pathology, Metabolome Facility of Bordeaux (MetaboHUB), Functional Genomics Center, IBVM, Centre INRA Bordeaux, Villenave d’Ornon, France.
    Rolin, Dominique
    INRA, Univ. Bordeaux, UMR1332 Fruit Biology and Pathology, Metabolome Facility of Bordeaux (MetaboHUB), Functional Genomics Center, IBVM, Centre INRA Bordeaux, Villenave d’Ornon, France.
    Dartigues, Benjamin
    Centre of bioinformatics of Bordeaux (CBiB), University of Bordeaux, Bordeaux, France.
    Conesa, Pablo
    European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus Hinxton, Cambridge, United Kingdom.
    Haug, Kenneth
    European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus Hinxton, Cambridge, United Kingdom.
    Rocca-Serra, Philippe
    University of Oxford e-Research Centre, Oxford, United Kingdom.
    O'Hagan, Steve
    School of Chemistry & Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom.
    Hao, Jie
    Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London, London, United Kingdom.
    van Vliet, Michael
    Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University, Leiden, Netherlands.
    Sysi-Aho, Marko
    Zora Biosciences OY, Espoo, Finland.
    Ludwig, Christian
    School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.
    Bouwman, Jildau
    Microbiology & Systems Biology TNO, Zeist, Netherlands.
    Cascante, Marta
    Department of Biochemistry and Molecular Biology, IBUB, Universitat de Barcelona, Barcelona, Spain.
    Ebbels, Timothy
    Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London, London, United Kingdom.
    Griffin, Julian L
    Medical Research Council Human Nutrition Research, Cambridge, United Kingdom; Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
    Moing, Annick
    INRA, UMR1332 Fruit Biology and Pathology, Metabolome Facility of Bordeaux (MetaboHUB), Functional Genomics Center (IBVM), Centre INRA Bordeaux Villenave d’Ornon, Univ. Bordeaux, Bordeaux, France.
    Nikolski, Macha
    University of Bordeaux CBiB/LaBRI, Bordeaux, France.
    Oresic, Matej
    Örebro University, School of Medical Sciences. Zora Biosciences OY, Espoo, Finland.
    Sansone, Susanna-Assunta
    University of Oxford e-Research Centre, Oxford, United Kingdom.
    Viant, Mark R.
    School of Biosciences, University of Birmingham Edgbaston, Birmingham, United Kingdom.
    Goodacre, Royston
    School of Chemistry & Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom.
    Günther, Ulrich L
    School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.
    Hankemeier, Thomas
    Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University, Leiden, Netherlands.
    Luchinat, Claudio
    Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino FI, Italy.
    Walther, Dirk
    Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
    Steinbeck, Christoph
    European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus Hinxton, Cambridge, United Kingdom.
    COordination of Standards in MetabOlomicS (COSMOS): facilitating integrated metabolomics data access2015In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 11, no 6, p. 1587-1597Article in journal (Refereed)
    Abstract [en]

    Metabolomics has become a crucial phenotyping technique in a range of research fields including medicine, the life sciences, biotechnology and the environmental sciences. This necessitates the transfer of experimental information between research groups, as well as potentially to publishers and funders. After the initial efforts of the metabolomics standards initiative, minimum reporting standards were proposed which included the concepts for metabolomics databases. Built by the community, standards and infrastructure for metabolomics are still needed to allow storage, exchange, comparison and re-utilization of metabolomics data. The Framework Programme 7 EU Initiative 'coordination of standards in metabolomics' (COSMOS) is developing a robust data infrastructure and exchange standards for metabolomics data and metadata. This is to support workflows for a broad range of metabolomics applications within the European metabolomics community and the wider metabolomics and biomedical communities' participation. Here we announce our concepts and efforts asking for re-engagement of the metabolomics community, academics and industry, journal publishers, software and hardware vendors, as well as those interested in standardisation worldwide (addressing missing metabolomics ontologies, complex-metadata capturing and XML based open source data exchange format), to join and work towards updating and implementing metabolomics standards.

  • 135.
    Sarwari, Wahida
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Optimering av realtids-PCR för identifiering och kvantifiering av Humant T-lymfotropt virus2013Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    Human T-lymfotropt virus (HTLV) är ett C-typ onkovirus som tillhör familjen Retroviridae som huvudsakligen angriper T-lymfocyter och orsakar leukemi och andra autoimmuna sjukdomar. I den nuvarande kliniska diagnostiken används Enzyme-linked immunosorbent assay och ibland vid screeningsmetoden uppträder ospecifika reaktioner. På senare tid har flera metoder baserade på real-tids PCR utvecklats för att bestämma halten av virala genom i patienter. Syftet med denna studie var att sätta upp och optimera en realtids-PCR för detektion av humant T-lymfotropt virus typ-1 och 2. Inför optimering av realtids-PCR extraherades DNA från MT-4 och MO-T cellinjer.  Under optimering av realtids-PCR användes SYBR Green och smältpunktsanalys där flera komponenter bl.a. templat-, primer-, magnesium- koncentrationer samt annealing/elongeringstemperaturen modifierades. Efter avslutad optimering utfördes probetitrering för att specifikt skilja ut HTLV-1 och HTLV-2. Amplifieringsprodukten verifierades med Sangersekvensering. För att hitta den lämpligaste metoden för extrahering av DNA från helblod testades olika extraheringsmetoder för DNA-preparering. Efter färdig optimering såg PCR-programmet ut enligt följande: 95ºC i 3 min följt av 45 cykler med 95ºC i 3s och 60ºC i 10s. De optimala koncentrationerna av de olika reagenserna var 0,5µM HTV-F5, 0,7µM HTV-R4, 0,2µM HTLV-P1 och 0,1µM HTLV-P2.  Den optimala extraktionsmetoden från helblod visades vara med MagNA Pure Compact med 500µL helblod. Den färdig optimerade PCR-metoden är en semi-kvanitativ metod som fungerar väl för detektion av HTLV 1 och 2, men vidareutveckling av metoden krävs innan den kan tas i bruk för klinisk diagnostik. 

  • 136.
    Scandurra, Isabella
    Uppsala universitet, Biomedicinsk informatik och teknik.
    Building Usability into Health Informatics: Development and Evaluation of Information Systems for Shared Homecare2007Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    How can we develop usable and work process-oriented ICT systems for shared homecare?

    Shared homecare involves different professionals, consists of mobile work and requires immediate and ubiquitous access to patient-oriented information, supporting an integrated view on the care process.

    This thesis presents a new collaborative design method for user needs analysis and requirements specification in the context of health information systems development; the Multi-disciplinary Thematic Seminar (MdTS) method. The thesis also describes the MdTS method’s application and two different usability evaluations of the developed system.

    The MdTS addresses a significant problem with health information technologies; they tend to support collaborative work of healthcare professionals poorly, sometimes leading to a fragmentation of workflow and disruption of healthcare processes. Based on human-computer interaction methods, MdTS implies a multiple-user needs analysis by thorough investigation of the entire interdisciplinary cooperative work and its transformation into technical specifications in order to develop appropriate information and communication technology (ICT) for the users’ differing work situations.

    Application of the MdTS resulted in a prototype, the OLD@HOME Virtual Health Record (VHR), adapted to the specific demands in shared homecare. Through mobile devices each care professional accessed patient information in profession-specific views from an integrated platform.

    This thesis provides an interesting case, illustrating how mobile ICT can support shared homecare, thereby bridging health and social care activities and improving knowledge about joint work processes.

    Results from the usability evaluations were overall positive. Information needed at point of care was available on mobile devices and presented in an understandable manner. However, the evaluations also indicated that it is difficult to transfer results from one homecare setting to another due to differences in operational routines.

    In conclusion, application of the MdTS method, in this study, succeeded in elicitation of correct user needs and in transferring correct requirements specifications to system developers for implementation.

  • 137.
    Sjölund, Fanny
    Örebro University, School of Health and Medical Sciences.
    Förhållandet mellan hudblodflöde och fysisk aktivitet.2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    SAMMAMFATTNING

    Bakgrund: Reaktiv hyperemi definieras som ett övergående ökat blodflöde över det normala efter en tids ischemi. Det kan registreras med laserdopplerteknik. Att registrera reaktiv hyperemi är ett sätt att värdera mikrocirkulationen. Det finns många flödesvariabler att studera varav tid till maxflöde efter ocklusion är en. Det har gjorts studier som undersöker om det finns ett samband mellan reaktiv hyperemi och fysisk aktivitet/syreupptagningsförmåga. Det har inte gjorts någon studie som undersöker tid till maxflöde och fysisk aktivitet. Syftet var att undersöka om det finns ett samband mellan fysisk aktivitet och reaktiv hyperemi med avseende på tid till maxflöde.

    Material och metod: Testpersoner fick bära en accelerometer en vecka under dygnets alla vakna timmar samt göra en registrering av reaktiv hyperemi med laserdoppler. För statistiska beräkningar användes oparat T-test för att undersöka skillnad mellan olika grad av fysisk aktivitet och tid till maxflöde.

    Resultat: Ingen statistiskt signifikant skillnad mellan olika aktivitetsgrad och tid till maxflöde kunde observeras.

    Slutsats: Den här studien visade inte på statistiskt signifikant samband mellan blodflöde och fysisk aktivitet.

  • 138.
    Sjöström, Ebba
    Örebro University, School of Health Sciences.
    Jämförelse mellan ImmuView och BinaxNow antigentest för detektion av Streptococcus pneumoniae och Legionella pneumophila i urin2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 139.
    Skröder, Sofia
    Örebro University, School of Health Sciences.
    Jämförelse av planar bildinsamling och kombinerad single photon emission computed tomography med datortomografi (SPECT/CT) hos patienter med frågeställning primär hyperparatyreoidism2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 140.
    Sohel Aboud, Aje
    Örebro University, School of Health Sciences.
    Jämförelse av total lungkapacitet mätt med kroppspletysmografi och heliumspädningsmetod En jämförelse av apparatur2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 141.
    Sterner, Sandra
    Örebro University, School of Health Sciences.
    Smittspårning av Yersinia enterocolitica och Yersinia pseudotuberculosis med Multilocus Variable-Number Tandem-Repeat Analysis2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 142.
    Torsson, Ylva
    Örebro University, School of Health Sciences.
    Förvaringsförhållandens effekt på urinprov från hund och katt2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 143.
    Törös, Bianca
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Genome-based characterization of Neisseria meningitidis with focus on the emergent serogroup Y disease2014Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Neisseria meningitidis, also referred to as meningococcus, is one of the leading causes of epidemic meningitis and septicaemia worldwide. Despite modern treatment, meningococcal disease remains associated with a high mortality (about 10%). Meningococcal disease is mainly restricted to specific hypervirulent lineages and specific capsular groups (serogroups), which have a changing global distribution over time. At the end of the 2000s, the previously unusual serogroup Y emerged, corresponding to half of all of the invasive meningococcal disease (IMD) cases in Sweden by the beginning of the 2010s. The aim of this thesis is to describe the emergence of serogroup Y meningococci genetically in an effort to understand some of the factors involved in the successful spread of this group throughout Sweden. In addition, genetic typing schemes were evaluated for surveillance and outbreak investigation.

    Our results indicate that the currently recommended typing for surveillance of meningococci could be altered to include the factor H-binding protein (fHbp). A highly variable multilocus variable number tandem repeat analysis (HV-MLVA) was able to confirm connected cases in a suspected small outbreak. In addition, a strain type sharing the same porA, fetA, porB, fHbp, penA and multilocus sequence type was found to be the principal cause of the increase in serogroup Y disease. However, a deeper resolution obtained from the core genomes revealed a subtype of this strain, which was mainly responsible for the increase. Finally, when the Swedish serogroup Y genomes were compared internationally, different strains seemed to dominate in different regions. This indicates that the increase was probably not due to one or more point introductions of a strain previously known internationally but more probably multifactorial.

    List of papers
    1. Evaluation of molecular typing methods for identification of outbreak-associated Neisseria meningitidis isolates
    Open this publication in new window or tab >>Evaluation of molecular typing methods for identification of outbreak-associated Neisseria meningitidis isolates
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    2013 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 121, no 6, p. 503-510Article in journal (Refereed) Published
    Abstract [en]

    It is essential in an outbreak investigation that strain characterization of Neisseria meningitidis is performed in a rapid and accurate manner. This study evaluated two new molecular typing methods, multiple- locus variable number tandem repeat analysis (MLVA) and repetitive sequence-based PCR (rep-PCR) (DiversiLab; bioMe´rieux) and compared them with current recommended methodologies. This retrospective study included 36 invasive N. meningitidis serogroup C isolates collected in Sweden 2001 through 2009 and previously subjected to outbreak investigation. All strains were typed with highly variable- MLVA (HV-MLVA) and rep-PCR. The isolates were further characterized by multilocus sequence typing (MLST) and sequencing of the fetA, fHbp, penA, porA and porB genes. The results showed that HVMLVA had the highest index of diversity (0.99) and rep-PCR had the highest congruence (40%) with the currently recommended typing methods. The HV MLVA correlated best to the spatiotemporal connections and had the overall highest Adjusted Wallace coefficients, suggesting that HV-MLVA can predict the results of the other typing methods in the study. We therefore suggest that after initial confirmation of species, serogroup and genosubtype, HV-MLVA should be used asthe most discriminatorymethod for first hand investigation of N. meningitidis serogroup C isolates.

    Keywords
    Neisseria meningitidis, molecular typing, repetitive sequence-based PCR, MLVA, epidemiology.
    National Category
    Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
    Research subject
    Microbiology
    Identifiers
    urn:nbn:se:oru:diva-36111 (URN)10.1111/apm.12022 (DOI)000319427100004 ()
    Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2019-03-26Bibliographically approved
    2. Genetic characterisation of the emerging invasive Neisseria meningitidis serogroup Y in Sweden, 2000 to 2010
    Open this publication in new window or tab >>Genetic characterisation of the emerging invasive Neisseria meningitidis serogroup Y in Sweden, 2000 to 2010
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    2011 (English)In: Eurosurveillance, ISSN 1025-496X, E-ISSN 1560-7917, Vol. 16, no 23, article id 19885Article in journal (Refereed) Published
    Abstract [en]

    Neisseria meningitidis serogroups B and C have beenresponsible for the majority of invasive meningococcaldisease in Europe. Recently, an increase of N. meningitidisdisease due to serogroup Y has been notedin Sweden (in 2010, the proportion was 39%, with anincidence of 0.23 per 100,000 population), as well as inother northern European countries. We aimed to investigatethe clonal pattern of the emerging serogroup Yin Sweden during 2000 to 2010. The serogroup Y isolatesidentified during this time (n=85) were characterisedby multilocus sequence typing and sequencing ofthe fetA, fHbp, penA, porA and porB genes. The mostfrequent clone (comprising 28 isolates) with identicalallele combinations of the investigated genes, waspartly responsible for the observed increased numberof N. meningitidis serogroup Y isolates. It was sulfadiazineresistant, with genosubtype P1.5-2,10-1,36-2,sequence type 23, clonal complex 23, porB allele 3-36,fetA allele F4-1, fHbp allele 25 and penA allele 22. Thefirst case with disease due to this clone was identifiedin 2002: there was a further case in 2004, six during2006 to 2007, eight during 2008 to 2009, with a peakof 12 cases in 2010. An unusual increase of invasivedisease in young adults (aged 20–29 years) caused bythis clone was shown, but no increase in mortality ratewas observed.

    Place, publisher, year, edition, pages
    Saint-Maurice, France: European Centre for the Epidemiological Monitoring of AIDS, 2011
    National Category
    Biomedical Laboratory Science/Technology
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-36113 (URN)000291586300002 ()21679677 (PubMedID)2-s2.0-79959480972 (Scopus ID)
    Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2019-03-26Bibliographically approved
    3. Surveillance of invasive Neisseria meningitidis with a serogroup Y update, Sweden 2010 to 2012
    Open this publication in new window or tab >>Surveillance of invasive Neisseria meningitidis with a serogroup Y update, Sweden 2010 to 2012
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    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    As previously described in this journal, an increase of invasive meningococcal disease caused by Neisseria meningitidis serogroup Y has been noted in Sweden, and to a lower extent throughout Europe. In the present study, we aimed to describe the current epidemiology of invasive N. meningitidis isolates in Sweden, with focus on the still increasing serogroup Y, and to find an optimal molecular typing scheme for both surveillance and outbreak investigations.

    All invasive N. meningitidis isolates in Sweden from 2010 to 2012 (n=208) were genetically characterized.

    The predominant serogroup in Sweden is still serogroup Y, in 2010, 2011 and 2012 corresponding to 22/57, 31/61 and 44/90of all invasive isolates (incidence 0.23, 0.33 and 0.46 per 100,000 population). Of the serogroup Y isolates in 2010, 2011 and 2012: 15/22, 23/32 and 19/44 were genetically clonal (Y: P1.5-2,10-1,36-2: F4-1: ST-23 (cc23), ‘porB allele 3- 36, fHbp allele 25 and penA allele 22), respectively. Our findings further support those of others that currently recommended FetA typing could be replaced by FHbp. Moreover, in line with our previous study, the current results indicate that highly variable multiple-locus variable number tandem repeat analysis (HV-MLVA) can be used as a first-hand rapid method for small outbreak investigations.

    National Category
    Biomedical Laboratory Science/Technology
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-36118 (URN)
    Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2017-10-17Bibliographically approved
    4. Whole-genome characterization of emergent invasive Neisseria meningitidis serogroup Y in Sweden from the two recent decades
    Open this publication in new window or tab >>Whole-genome characterization of emergent invasive Neisseria meningitidis serogroup Y in Sweden from the two recent decades
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    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    Background and Objective: Invasive meningococcal disease (IMD) caused by Neisseria meningitidis serogroup Y has increased in Europe, especially in Scandinavia. In Sweden, serogroup Y is the dominating serogroup and in 2012 the serogroup Y disease incidence was 0.46/100,000 population. We have previously shown that a strain type belonging to ST-23 is responsible for the emergence of this serogroup in Sweden. The objective of this study was to compare the meningococcal population structure and phylogeography of Swedish invasive serogroup Y strains to other countries with different disease incidence.

    Materials and Methods: Whole-genome sequencing was performed on invasive serogroup Y isolates from 1995 to 2012 in Sweden (n=186). A comparison of serogroup Y isolates was performed using a collection of isolates from England, Wales and Northern Ireland (n=143), which has relatively low incidence, and two isolates from the USA, where serogroup Y remains one of the major causes of IMD.

    Results: The meningococcal population structures were similar in the investigated regions; however, different strain types were dominating in each geographic region. A number of genes, known or hypothesized to have an impact on meningococcal virulence, were shown to be associated with different strain types and subtypes.

    Conclusions: The emergence of serogroup Y is most likely not associated with a previously described strain type that has been introduced into the Swedish meningococcal population. The reasons for the disease increase are most probably multifactorial; both increased virulence and host adaptive immunity influence infection and transmission. Future genomewide association studies could reveal additional genes associated with serogroup Y meningococcal disease.

    Keywords
    Neisseria meningitidis, genome sequencing, epidemiology, serogroup Y, invasive meningococcal disease
    National Category
    Biomedical Laboratory Science/Technology
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-36120 (URN)
    Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2017-10-17Bibliographically approved
  • 144.
    Törös, Bianca
    et al.
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Hedberg, Sara [Thulin]
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Jacobsson, Susanne
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Fredlund, Hans
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Olcén, Per
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Mölling, Paula
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Surveillance of invasive Neisseria meningitidis with a serogroup Y update, Sweden 2010 to 2012Manuscript (preprint) (Other academic)
    Abstract [en]

    As previously described in this journal, an increase of invasive meningococcal disease caused by Neisseria meningitidis serogroup Y has been noted in Sweden, and to a lower extent throughout Europe. In the present study, we aimed to describe the current epidemiology of invasive N. meningitidis isolates in Sweden, with focus on the still increasing serogroup Y, and to find an optimal molecular typing scheme for both surveillance and outbreak investigations.

    All invasive N. meningitidis isolates in Sweden from 2010 to 2012 (n=208) were genetically characterized.

    The predominant serogroup in Sweden is still serogroup Y, in 2010, 2011 and 2012 corresponding to 22/57, 31/61 and 44/90of all invasive isolates (incidence 0.23, 0.33 and 0.46 per 100,000 population). Of the serogroup Y isolates in 2010, 2011 and 2012: 15/22, 23/32 and 19/44 were genetically clonal (Y: P1.5-2,10-1,36-2: F4-1: ST-23 (cc23), ‘porB allele 3- 36, fHbp allele 25 and penA allele 22), respectively. Our findings further support those of others that currently recommended FetA typing could be replaced by FHbp. Moreover, in line with our previous study, the current results indicate that highly variable multiple-locus variable number tandem repeat analysis (HV-MLVA) can be used as a first-hand rapid method for small outbreak investigations.

  • 145.
    Törös, Bianca
    et al.
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Hedberg, Sara [Thulin]
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Unemo, Magnus
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Jacobsson, Susanne
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Hill, Dorothea M.C.
    Department of Zoology, University of Oxford, Oxford, United Kingdom.
    Olcén, Per
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Fredlund, Hans
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Bratcher, Holly B.
    Department of Zoology, University of Oxford, Oxford, United Kingdom.
    Jolley, Keith A.
    Department of Zoology, University of Oxford, Oxford, United Kingdom.
    Maiden, Martin C.J.
    Department of Zoology, University of Oxford, Oxford, United Kingdom.
    Mölling, Paula
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Whole-genome characterization of emergent invasive Neisseria meningitidis serogroup Y in Sweden from the two recent decadesManuscript (preprint) (Other academic)
    Abstract [en]

    Background and Objective: Invasive meningococcal disease (IMD) caused by Neisseria meningitidis serogroup Y has increased in Europe, especially in Scandinavia. In Sweden, serogroup Y is the dominating serogroup and in 2012 the serogroup Y disease incidence was 0.46/100,000 population. We have previously shown that a strain type belonging to ST-23 is responsible for the emergence of this serogroup in Sweden. The objective of this study was to compare the meningococcal population structure and phylogeography of Swedish invasive serogroup Y strains to other countries with different disease incidence.

    Materials and Methods: Whole-genome sequencing was performed on invasive serogroup Y isolates from 1995 to 2012 in Sweden (n=186). A comparison of serogroup Y isolates was performed using a collection of isolates from England, Wales and Northern Ireland (n=143), which has relatively low incidence, and two isolates from the USA, where serogroup Y remains one of the major causes of IMD.

    Results: The meningococcal population structures were similar in the investigated regions; however, different strain types were dominating in each geographic region. A number of genes, known or hypothesized to have an impact on meningococcal virulence, were shown to be associated with different strain types and subtypes.

    Conclusions: The emergence of serogroup Y is most likely not associated with a previously described strain type that has been introduced into the Swedish meningococcal population. The reasons for the disease increase are most probably multifactorial; both increased virulence and host adaptive immunity influence infection and transmission. Future genomewide association studies could reveal additional genes associated with serogroup Y meningococcal disease.

  • 146.
    Uporova, Ludmila
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Betydelsen av polyformismen rs2254958 i Protein Kinas R (PKR/EIF2AK2) genen för utveckling av inflammatorisk tarmsjukdom (IBD)2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 147.
    Wallin, Sara
    Örebro University, School of Health Sciences.
    Användande av Microwave HistoSTATION vid urkalkning av histologiska preparat: En jämförelse med konventionell teknik2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 148.
    Wallin, Sofia
    Örebro University, School of Health and Medical Sciences.
    Effekten av 5 veckors utövande av yoga och meditation på ambulatoriskt blodtryck och mikrocirkulatoriskt hudblodflöde2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 149.
    Wennerholm, Julia
    Örebro University, School of Health Sciences.
    Utvärdering av IRIS iQ200 för screening av urinprover innan odling2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 150.
    Wilenius, Kristin
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Retrospective evaluation of the diagnostic accuracy of FluoroType® MTB assay used for detection of Mycobacterium tuberculosis in clinical samples in Greece2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
1234 101 - 150 of 151
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