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  • 101.
    Fan, Chenjing
    Örebro universitet, Institutionen för läkarutbildning.
    Pilot study of speckle-type POZprotein as a potential diagnosticmarker for prostate cancer2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 102.
    Fart, Frida
    Örebro universitet, Institutionen för läkarutbildning.
    Effects of habitual exercise on saliva immunoglobulin A in older adults2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 103.
    Faxberg, Carl
    Örebro universitet, Institutionen för läkarutbildning.
    Is the anti-thrombotic effect of angiotensin 1-7 a consequence of endothelial nitric oxideproduction?2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 104.
    Forsman, Cecilia
    Örebro universitet, Institutionen för läkarutbildning.
    "Säröbomber" and the effect on sleep in patients with dementia2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 105.
    Forsman, Cecilia
    Örebro universitet, Institutionen för läkarutbildning.
    The prevalence of overweight and obesity in children and adolescents with Attention Deficit/Hyperactive Disorder2015Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
  • 106.
    Forsman, Daniel
    Örebro universitet, Institutionen för läkarutbildning.
    Expression of CD24 and CD44 in breast cancer cells after cytotoxic drug exposure2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 107.
    Forsman Ekendahl, Jessica
    Örebro universitet, Institutionen för läkarutbildning.
    Uttryck av somatostatinreceptor typ 2 på bröstkarinomceller och makrofager2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 108.
    Fransson, Kalle
    Örebro universitet, Institutionen för läkarutbildning.
    DEXAMETHASONE AND RANIBIZUMAB AS TREATMENT FOR CENTRAL RETINAL VEIN OCCLUSION: A quality assessment of the efficacy of Dexamethasone and Ranibizumab following a central retinal vein occlusion at the department of Ophthalmology, Örebro University Hospital2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 109.
    Fransén, Karin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Franzén, Petra
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Magnuson, Anders
    Örebro University Hospital, Örebro, Sweden.
    Elmabsout, Ali
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Nyhlin, Nils
    Region Örebro län.
    Wickbom, Anna
    Örebro University Hospital, Örebro, Sweden.
    Curman, Bengt
    Örebro University Hospital, Örebro, Sweden.
    Törkvist, Leif
    Karolinska University Hospital, Stockholm, Sweden.
    D'Amato, Mauro
    Karolinska University Hospital, Stockholm, Sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Sirsjö, Allan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Polymorphism in the retinoic acid metabolizing enzyme CYP26B1 and the development of Crohn's disease2013Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, nr 8, s. e72739-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Several studies suggest that Vitamin A may be involved in the pathogenesis of inflammatory bowel disease (IBD), but the mechanism is still unknown. Cytochrome P450 26 B1 (CYP26B1) is involved in the degradation of retinoic acid and the polymorphism rs2241057 has an elevated catabolic function of retinoic acid, why we hypothesized that the rs2241057 polymorphism may affect the risk of Crohn's disease (CD) and Ulcerative Colitis (UC). DNA from 1378 IBD patients, divided into 871 patients with CD and 507 with UC, and 1205 healthy controls collected at Örebro University Hospital and Karolinska University Hospital were analyzed for the CYP26B1 rs2241057 polymorphism with TaqMan® SNP Genotyping Assay followed by allelic discrimination analysis. A higher frequency of patients homozygous for the major (T) allele was associated with CD but not UC compared to the frequency found in healthy controls. A significant association between the major allele and non-stricturing, non-penetrating phenotype was evident for CD. However, the observed associations reached borderline significance only, after correcting for multiple testing. We suggest that homozygous carriers of the major (T) allele, relative to homozygous carriers of the minor (C) allele, of the CYP26B1 polymorphism rs2241057 may have an increased risk for the development of CD, which possibly may be due to elevated levels of retinoic acid. Our data may support the role of Vitamin A in the pathophysiology of CD, but the exact mechanisms remain to be elucidated.

  • 110.
    Franzén, Karin M
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Obstetrics and Gynecology, Örebro University Hospital, Örebro, Sweden.
    Andersson, Gunnel
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Odeberg, Jenny
    Swedish Council on Health Technology Assessment (SBU), Stockholm, Sweden.
    Midlöv, Patrik
    Center for Primary Health Care Research, Lund University, Malmö, Sweden.
    Samuelsson, Eva
    Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden.
    Stenzelius, Karin
    Department of Care Science, Malmö University, Malmö, Sweden.
    Hammarström, Margareta
    Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm, Sweden.
    Surgery for urinary incontinence in women 65 years and older: a systematic review2015Inngår i: International Urogynecology Journal, ISSN 0937-3462, E-ISSN 1433-3023, Vol. 26, nr 8, s. 1095-1102Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Introduction and hypothesis: Urinary incontinence (UI) is common among the elderly, but the literature is sparse on the surgical treatment of UI among the elderly. This systematic review aims to assess the effectiveness of surgical interventions as treatment for urinary incontinence in the elderly population ≥65 years of age.

    Methods: Randomized controlled trials (RCT) and prospective nonrandomized studies (NRS) were included. The databases PubMed (NLM), EMBASE (Elsevier), Cochrane Library (Wiley), and Cinahl (EBSCO) were searched for the period 1966 up to October 2013. The population had to be ≥65 years of age and had to have undergone urethral sling procedures, periurethral injection of bulking agents, artificial urinary sphincter surgery, bladder injection treatment with onabotulinumtoxin A or sacral neuromodulation treatment. Eligible outcomes were episodes of incontinence/urine leakage, adverse events, and quality of life.

    The studies included had to be at a moderate or low risk of bias. Mean difference (MD) or standard mean difference (SMD)as well as risk difference (RD) and the 95 % CI were calculated.

    Results: Five studies-all on the suburethral sling procedure in women- that fulfilled the inclusion criteria were identified. The proportion of patients reporting persistent SUI after surgery ranged from 5.2 to 17.6 %. One study evaluating quality of life (QoL) showed a significant improvement after surgery. The complication rates varied between 1 and 26 %, mainly bladder perforation, bladder emptying disturbances, and de novo urge.

    Conclusion: The suburethral sling procedure improves continence as well as QoL among elderly women with SUI; however, evidence is limited.

  • 111.
    Fälker, Knut
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Biomedicine; Dept Clin & Expt Med, Linköping Univ, Linköping, Sweden.
    Klarström-Engström, Kristin
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Biomedicine.
    Bengtsson, Torbjörn
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Biomedicine.
    Lindahl, Tomas L.
    Dept Clin & Expt Med, Linköping Univ, Linköping, Sweden.
    Grenegård, Magnus
    Örebro universitet, Institutionen för läkarutbildning. Department of Biomedicine.
    The Toll-like receptor 2/1 (TLR2/1) complex initiates human platelet activation via the src/Syk/LAT/PLC gamma 2 signalling cascade2014Inngår i: Cellular Signalling, ISSN 0898-6568, E-ISSN 1873-3913, Vol. 26, nr 2, s. 279-286Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The specific TLR2/1 complex activator Pam3CSK4 has been shown to provoke prominent activation and aggregation of human non-nucleated platelets. As Pam3CSK4-evoked platelet activation does not employ the major signalling pathway established in nucleated immune cells, we investigated if the TLR2/1 complex on platelets may initiate signalling pathways known to be induced by physiological agonists such as collagen via GPVI or thrombin via PARs. We found that triggering TLR2/1 complex-signalling with Pam3CSK4, in common with that induced via GPVI, and in contrast to that provoked by PARS, involves tyrosine phosphorylation of the adaptor protein LAT as well as of PLC gamma 2 in a src- and Syk-dependent manner. In this respect, we provide evidence that Pam3CSK4 does not cross-activate GPVI. Further, by the use of platelets from a Glanzmann's thrombasthenia patient lacking beta(3), in contrast to findings in nucleated immune cells, we show that the initiation of platelet activation by Pam3CSK4 does not involve integrin beta(3) signalling; whereas the latter, subsequent to intermediate TXA2 synthesis and signalling, was found to be indispensable for proper dense granule secretion and full platelet aggregation. Together, our findings reveal that triggering the TLR2/1 complex with Pam3CSK4 initiates human platelet activation by engaging tyrosine kinases of the src family and Syk, the adaptor protein LAT, as well as the key mediator PLC gamma 2. (C) 2013 Elsevier Inc. All rights reserved.

  • 112.
    Gabrielson, Marike
    et al.
    School of Health and Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Reizer, Edwin
    School of Health and Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Stål, Olle
    Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Oncology, Linköping University, Linköping, Sweden.
    Tina, Elisabet
    Örebro universitet, Institutionen för läkarutbildning. Department of Clinical Research Laboratory.
    Mitochondrial regulation of cell cycle progression through SLC25A432016Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 469, nr 4, s. 1090-1096Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    An increasing body of evidence is pointing towards mitochondrial regulation of the cell cycle. In a previous study of HER2-positive tumours we could demonstrate a common loss in the gene encoding for the mitochondrial transporter SLC25A43 and also a significant relation between SLC25A43 protein expression and S-phase fraction. Here, we investigated the consequence of suppressed SLC25A43 expression on cell cycle progression and proliferation in breast epithelial cells.

    In the present study, we suppressed SLC25A43 using siRNA in immortalised non-cancerous breast epithelial MCF10A cells and HER2-positive breast cancer cells BT-474. Viability, apoptosis, cell proliferation rate, cell cycle phase distribution, and nuclear Ki-67 and p21, were assessed by flow cytometry. Cell cycle related gene expressions were analysed using real-time PCR.

    We found that SLC25A43 knockdown in MCF10A cells significantly inhibited cell cycle progression during G(1)-to-S transition, thus significantly reducing the proliferation rate and fraction of Ki-67 positive MCF10A cells. In contrast, suppressed SLC25A43 expression in BT-474 cells resulted in a significantly increased proliferation rate together with an enhanced G(1)-to-S transition. This was reflected by an increased fraction of Ki-67 positive cells and reduced level of nuclear p21. In line with our previous results, we show a role for SLC25A43 as a regulator of cell cycle progression and proliferation through a putative mitochondrial checkpoint. These novel data further strengthen the connection between mitochondrial function and the cell cycle, both in non-malignant and in cancer cells. (C) 2015 Elsevier Inc. All rights reserved.

  • 113.
    Gnosa, S.
    et al.
    County Council of Östergötland, Linköping, Sweden; University of Linköping, Linköping, Sweden.
    Zhang, Hong
    Örebro universitet, Institutionen för läkarutbildning.
    Brodin, V. P.
    County Council of Östergötland, Linköping, Sweden; University of Linköping, Linköping, Sweden.
    Carstensen, J.
    Linköping University, Linköping, Sweden.
    Adell, G.
    Karolinska University Hospital, Stockholm, Sweden.
    Sun, X.-F.
    County Council of Östergötland, Linköping, Sweden; University of Linköping, Linköping, Sweden.
    AEG-1 expression is an independent prognostic factor in rectal cancer patients with preoperative radiotherapy: a study in a Swedish clinical trial2014Inngår i: British Journal of Cancer, ISSN 0007-0920, E-ISSN 1532-1827, Vol. 111, nr 1, s. 166-173Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Preoperative radiotherapy (RT) is widely used to downstage rectal tumours, but the rate of recurrence varies significantly. Therefore, new biomarkers are needed for better treatment and prognosis. It has been shown that astrocyte elevated gene-1 (AEG-1) is a key mediator of migration, invasion, and treatment resistance. Our aim was to analyse the AEG-1 expression in relation to RT in rectal cancer patients and to test its radiosensitising properties.

    Methods: The AEG-1 expression was examined by immunohistochemistry in 158 patients from the Swedish clinical trial of RT. Furthermore, we inhibited the AEG-1 expression by siRNA in five colon cancer cell lines and measured the survival after irradiation by colony-forming assay.

    Results: The AEG-1 expression was increased in the primary tumours compared with the normal mucosa independently of the RT (P<0.01). High AEG-1 expression in the primary tumour of the patients treated with RT correlated independently with higher risk of distant recurrence (P = 0.009) and worse disease-free survival (P = 0.007). Downregulation of AEG-1 revealed a decreased survival after radiation in radioresistant colon cancer cell lines.

    Conclusions: The AEG-1 expression was independently related to distant recurrence and disease-free survival in rectal cancer patients with RT and could therefore be a marker to discriminate patients for distant relapse.

  • 114.
    Gnosa, Sebastian
    et al.
    Linköping University, Linköping, Sweden.
    Brodin, Veronika Patcha
    Linköping University, Linköping, Sweden.
    Ticha, Ivana
    Linköping University, Linköping, Sweden.
    Adell, Gunnar
    Linköping University, Linköping, Sweden.
    Arbman, Gunnar
    Vrinnevi Hospital, Norrköping, Sweden.
    Zhang, Hong
    Örebro universitet, Institutionen för läkarutbildning.
    Sun, Xiao-Feng
    Linköping University, Linköping, Sweden.
    Genetic variants and expression of AEG-1 in relation to clinical outcome and radiotherapy response in colorectal cancer patients and cell lines2014Inngår i: Cancer Research, ISSN 0008-5472, E-ISSN 1538-7445, Vol. 74, nr 19Artikkel i tidsskrift (Annet vitenskapelig)
  • 115.
    Grahn, Julia
    Örebro universitet, Institutionen för läkarutbildning.
    Fecal transplantation as a treatment for recurrent Clostridium difficile infection: a follow-up on patients treated at the Infections clinic at Örebro University Hospital2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 116.
    Grundell, Maja
    Örebro universitet, Institutionen för läkarutbildning.
    Förekomst av Helicobacter pylori, Hepatit B-antikroppar samt ABO-tillhörighet hos en högriskpopulation för ventrikelcancer i Nicaragua2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 117.
    Gunaltay, Sezin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Ghiboub, M.
    Frenche Comte Univ, Besancon, France.
    Hultgren, Olof
    Örebro universitet, Institutionen för läkarutbildning.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    The role of IL-37 on cytokine responses downstream of TLR4 and TLR5 signalling in intestinal epithelial cells2014Inngår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 143, s. 94-95Artikkel i tidsskrift (Annet vitenskapelig)
  • 118.
    Gunaltay, Sezin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kumawat, Ashok Kumar
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Institute of Infection, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, United Kingdom.
    Nyhlin, Nils
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Tysk, Curt
    Region Örebro län. Division of Gastroenterology, Department of Medicine, Örebro University, Örebro, Sweden.
    Hultgren, Olof
    Region Örebro län.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Enhanced levels of chemokines and their receptors in the colon of microscopic colitis patients indicate mixed immune cell recruitment2015Inngår i: Mediators of Inflammation, ISSN 0962-9351, E-ISSN 1466-1861, artikkel-id 132458Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Microscopic colitis (MC), comprising collagenous colitis (CC) and lymphocytic colitis (LC), is a common cause of chronic diarrhea. Various immune cell infiltrations in the epithelium and lamina propria are seen in MC immunopathology. We compared gene and protein expressions of different immune cell attracting chemokines and their receptors in colon biopsies from MC patients in active disease or histopathological remission (CC/LC-HR) with controls, using qRT-PCR and Luminex, respectively. CC and LC patients with active disease demonstrated a mixed chemokine profile with significantly enhanced gene and/or protein expressions of the chemokines CCL2, CCL3, CCL4, CCL5, CCL7, CCL22, CXCL8, CXCL9, CXCL10, CXCL11, and CX(3)CL1 and the receptors CCR2, CCR3, CCR4, CXCR1, CXCR2, and CX(3)CR1. Enhanced chemokine/chemokine receptor gene and protein levels in LC-HR patients were similar to LC patients, whereas CC-HR patients demonstrated almost normalized levels. These findings expand the current understanding of the involvement of various immune cells in MC immunopathology and endorse chemokines as potential diagnostic markers as well as therapeutic candidates. Moreover, this study further supports the hypothesis that CC and LC are two different entities due to differences in their immunoregulatory responses.

  • 119.
    Gunaltay, Sezin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Nyhlin, Nils
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kumawat, Ashok
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro University Hospital, Örebro, Sweden.
    Hultgren, Olof
    Örebro universitet, Institutionen för läkarutbildning.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Increased expression of T cell recruiting chemokines in the colonic mucosa of microscopic colitis patients2013Inngår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 140, s. 135-135Artikkel i tidsskrift (Annet vitenskapelig)
  • 120.
    Gunaltay, Sezin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Nyhlin, Nils
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kumawat, Ashok
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren, Olof
    Örebro universitet, Institutionen för läkarutbildning.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    IL-1/TLR signaling inhibitors in microscopic and ulcerative colitis: Immunopathogenic markers of active disease and remission2013Inngår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 140, s. 167-167Artikkel i tidsskrift (Annet vitenskapelig)
  • 121.
    Gunnarsson, Lars-Gunnar
    et al.
    Örebro universitet, Institutionen för läkarutbildning.
    Bodin, Lennart
    Epidemiologiskt påvisade samband mellan Parkinsons sjukdom och faktorer i arbetsmiljön2014Inngår i: Systematiska kunskapsöversikter: 6. Epidemiologiskt påvisade samband mellan Parkinsons sjukdom och faktorer i arbetsmiljön / [ed] Lars-Gunnar Gunnarsson och Lennart Bodin, Göteborg: Göteborgs universitet , 2014, s. 11-79Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    Epidemiological evidence on associations between work environment and Parkinson’s disease.

    Lars-Gunnar Gunnarsson, assistant professor at School of Medicine, Örebro University, Sweden and

    Lennart Bodin, professor emeritus, Department of Statistics, Örebro University, Sweden and senior researcher at Institute of Environmental Medicine, Karolinska Institute, Sweden.

    In this systematic literature review we identified 93 original publications on associations between work and Parkinson’s disease (PD). GRADE guidelines were used to secure high scientific quality and reliable guidelines were applied to classify the papers. Forty-nine papers fulfilled high quality standards while 44 were methodologically deficient and thus were excluded from our meta-analyses.

    Twenty five publications concerned work exposure to pesticides. The weighted relative risk (RR) estimate was 1.72 (95% confidence interval 1.46-2.04). A funnel plot and tests indicated that some publication bias concerning smaller studies might have been present. The risk estimate was not influenced by study design (case-control, cohort or cross section study) or gender. Higher estimates were found when there was a hereditary taint or onset below age 60.

    In 15 publications exposure to electromagnetic fields was considered and there was no indication of risk, RR 1.07 (95% confidence interval 0.98-1.18).

    Meta-analyses of ten studies on exposure for metals showed a statistically diminished risk estimate, RR 0.85 (95% confidence interval 0.82-0.89). A proposed explanation to this seemingly beneficial effect is hormesis meaning that exposures of a low-moderate dosage of toxic agents can induce neuroprotective mechanisms.

     

  • 122.
    Gunnarsson, Lars-Gunnar
    et al.
    Örebro universitet, Institutionen för läkarutbildning.
    Bodin, Lennart
    Örebro universitet, Handelshögskolan vid Örebro Universitet. Karolinska institutet, Stockholm, Sweden.
    Systematiska kunskapsöversikter;: 7. Epidemiologiskt påvisade samband mellan Amyotrofisk Lateral Skleros (ALS) och faktorer i arbetsmiljön2014Rapport (Fagfellevurdert)
    Abstract [sv]

    Metod

    Vid systematisk litteraturgenomgång identifierade vi 61 relevanta epidemiologiska publikationer med originaldata över samband mellan ALS och exponeringar i arbetslivet. Samtliga artiklar granskades och 34 av dessa studier uppfyllde kriterierna för god vetenskaplig kvalitet

    Resultat

    Bekämpningsmedel och andra kemikalier har i sju studier värderats med hjälp av fråge­formulär/intervjuer. Våra metaanalyser visade att riskskattningarna var något högre för fall-kontrollstudier än för kohortstudier (1,82 respektive 1,47) och sammantaget blev riskmåttet 1,67 (95% konfidensintervall 1,14-2,44). Höga och statistiskt signifikant ökade risker rapporteras i de tre studier som fokuserade på jordbruksarbete; riskmått 2,4-4,7. Ett möjligt samband finns också mellan ALS och yrkesmässig exponering för metaller.

    Tungt och långvarigt muskelarbete ofta i kombination med muskeltrauma kunna öka risken för ALS med RR runt 2,0. Till denna risk grupp hör professionella fotbolls­spelare i europeisk och amerikansk fotboll. Däremot verkar inte fysisk aktivitet i arbetet och på fritiden påverka risken för att drabbas av ALS.

    I 14 metodologiskt relevanta publikationer granskas eventuellt samband mellan ALS och exponering för elektromagnetiska fält (EMF) och/eller arbete med elektriskt utrustning. Våra egna metaanalyser samt tre publicerade metaanalyser indikerade en låg riskökning. Samman­vägd analys av exponeringar och resultat indikerar att också andra exponeringar än EMF skulle kunna förklara sambandet, t ex muskeltrauma till följd av elstötar.

    Diskussion

    Longitudinella epidemiologiska studier indikerar att ALS huvudsakligen drabbar personer som har en ökad sårbarhet (en genetisk disposition) för att sjukdomsprocessen skall starta. I kombination med olika exponeringar blir nervcellens sjukdomsbörda för stor så att sjukdomsprocessen startar.

  • 123.
    Gunnarsson, Linus
    Örebro universitet, Institutionen för läkarutbildning.
    Exposure to respirable dust and cardiovascular disease mortality among Swedish iron foundry workers2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 124.
    Gunnarsson, Martin
    et al.
    Örebro universitet, Institutionen för läkarutbildning.
    Udumyan, Ruzan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bahmanyar, S.
    Clinical Epidemiology Unit and Centre for Pharmacoepidemiology, Department of Medicine, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden; Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran .
    Nilsagård, Ylva
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Montgomery, Scott
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Clinical Epidemiology Unit, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden; Research Department of Epidemiology and Public Health, University College London, London, United Kingdom .
    Characteristics in childhood and adolescence associated with future multiple sclerosis risk in men: cohort study2015Inngår i: European Journal of Neurology, ISSN 1351-5101, E-ISSN 1468-1331, Vol. 22, nr 7, s. 1131-1137Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and purpose: Associations with multiple sclerosis (MS) of living conditions in childhood and characteristics in adolescence including physical fitness, cognitive function and psychological stress resilience were investigated.

    Methods: A cohort of male Swedish residents born 1952-1956 who were included in the Swedish Military Conscription Register was used to create a nested case-control study comprising 628 MS cases and 6187 controls matched on birth year, county of residence and vital status at time of diagnosis. Conscription examination records were linked with other national register data. Conditional logistic regression was used to evaluate associations with MS subsequent to the conscription examination.

    Results and conclusions: Men with MS were less likely to be from more crowded households in childhood (>two persons per room) with an adjusted odds ratio of 0.67 (95% confidence interval 0.51-0.86, P=0.023). They had lower physical working capacity in adolescence with adjusted odds ratio of 0.94 (95% confidence interval 0.89-0.99, P=0.026). Cognitive function and stress resilience scores displayed no significant differences between cases and controls. Parental occupation in childhood and body mass index in adolescence were not associated with future MS risk. The inverse association of MS risk with higher levels of household crowding may reflect environmental factors such as the pattern of exposure to microorganisms. Lower physical fitness in men at MS risk may indicate a protective effect of exercise or could be due to prodromal disease activity, although there was no association with cognitive function. Poor psychological stress resilience (and thus risk of chronic stress arousal) was not associated with MS.

  • 125.
    Gustafsson, Ulf O.
    et al.
    Department of Surgery, Ersta Hospital, Karolinska Institutet, Stockholm, Sweden.
    Segelman, Josefin
    Department of Molecular Medicine and Surgery, Ersta Hospital, Karolinska University Hospital, Stockholm, Sweden.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Surgery, Örebro University Hospital, Örebro, Sweden.
    Thorell, Anders
    Department of Surgery, Ersta Hospital, Karolinska Institutet, Stockholm, Sweden.
    Nygren, Jonas
    Department of Surgery, Ersta Hospital, Karolinska Institutet, Stockholm, Sweden.
    Can nutritional supplements and rectal enema be used as bowel cleansing for colonoscopy?: results of a randomized controlled pilot study2014Inngår i: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 49, nr 4, s. 485-491Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective. Currently available preparations for colonoscopy have low tolerability and may cause fluid and electrolyte shifts. An alternative method of bowel cleansing is required.

    Material and methods. Preparation of the gut using oral nutritional supplements (ONS) and rectal enema was tested as an alternative method of bowel cleansing. During 2008-2012, patients were randomized to oral nutritional supplements (n = 27) for 5 days and rectal enema or polyethylene glycol (PEG) (n = 23) prior to colonoscopy. Blinded endoscopists rated the degree of bowel cleansing according to the Ottawa bowel preparation scale (OBS) (primary outcome). Tolerability of either preparation was also assessed (ClinicalTrials.gov. Identifier no: NCT00123456).

    Results. Due to a high rate of bowel cleansing failure among patients receiving ONS, the study was interrupted prematurely. Colonoscopies were incomplete due to stools in 6 of 27 patients in the ONS group compared to 1 of 23 in the PEG group (ns). The mean total OBS were 8.3 +/- 3.3 and 5.3 +/- 2.8, respectively (p = 0.002). Four patients (15%) in the ONS group and eight patients (35%) receiving PEG had an OBS score <= 4 (good preparation) (ns). ONS was better tolerated than PEG with more patients reporting acceptable taste (27 of 27 [100%] vs. 15 of 23 [65%], p = 0.001), and fewer reporting difficulties with the intake (0 of 27 [0%] vs. 10 of 23 [43%], p < 0.001) and nausea (5 of 27 [19%] vs. 13 of 23 [57%], p < 0.008).

    Conclusions. For routine use, ONS with enema instead of traditional preparation for colonoscopy with PEG cannot be generally recommended.

  • 126. Gustavsson, Anders
    et al.
    Jaenerot, Gunnar
    Hertervig, Erik
    Friis-Iiby, Ingalill
    Blomquist, Lars
    Karlen, Per
    Granno, Christer
    Vilien, Mogens
    Strom, Magnus
    Damelsson, Ake
    Verbaan, Hans
    Hellstrom, Per M.
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning.
    Magnuson, Anders
    Tysk, Curt
    A 2-year follow-up of the swedish-danish Infliximab/Placebo trial in steroid resistant acute ulcerative colitis2007Inngår i: Gastroenterology, ISSN 0016-5085, E-ISSN 1528-0012, Vol. 132, nr 4, s. A146-A147Artikkel i tidsskrift (Annet vitenskapelig)
  • 127. Gustavsson, Anders
    et al.
    Magnuson, Anders
    Blomberg, Björn
    Andersson, Magnus V.
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning.
    Tysk, Curt
    Endoscopic Balloon Dilation of Intestinal Strictures in Crohn's Disease2011Inngår i: Gastroenterology, ISSN 0016-5085, E-ISSN 1528-0012, Vol. 140, nr 5, s. S140-S140Artikkel i tidsskrift (Annet vitenskapelig)
  • 128.
    Göranzon, C.
    et al.
    Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Kumawat, Ashok Kumar
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren-Hörnqvist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Eriksson, S.
    Department of Pathology, Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    School of Health and Medical Sciences, Örebro University, Örebro, Sweden; Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Nyhlin, Nils
    Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Immunohistochemical characterization of lymphocytes in microscopic colitis2013Inngår i: Journal of Crohn's and Colitis, ISSN 1197-4982, Vol. 7, nr 10, s. e434-e442Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and Aims: Microscopic colitis (MC), encompassing the subgroups collagenous colitis (CC) and lymphocytic colitis (LC), is characterized by macroscopically normal or near-normal colonic mucosa, and an increased number of intraepithelial lymphocytes (IELs) and mononuclear cell infiltration in the underlying lamina propria (LP), in addition to an increased collagen layer in CC. This study aimed to characterize the inflammatory cells involved in mucosal inflammation, using immunohistochemistry.

    Methods Paraffin-embedded biopsies from 23 untreated patients with MC (CC = 13, LC = 10) and 17 controls were stained with antibodies against CD3, CD4, CD8, CD20, CD30, Foxp3, CD45RO and Ki67. Computerized image analysis was used to calculate areas of stained lymphocytes in the surface and crypt epithelia as well as in the LP.

    Results In CC and LC, an increase of predominantly CD8+ lymphocytes was seen in both the epithelium and the lamina propria, whereas a decreased amount of CD4+ lymphocytes was found in the lamina propria. CD45RO+ and Foxp3+ cells were more abundant in all areas in both patient groups compared to controls, as were CD20+ areas, although more scarce. Ki67+ areas were only more abundant in the epithelium, whereas CD30+ areas were more abundant in the lamina propria of both patient groups compared to controls.

    Conclusions This study confirms an increased amount of CD8+ lymphocytes in the epithelium. Lymphocytic proliferation and activation markers were more abundant, whereas a decreased amount of CD4+ lymphocytes was seen in the LP. Further studies are needed to reveal the underlying mechanism(s).

  • 129.
    Göthlin Eremo, Anna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tina, Elisabet
    Örebro University Hospital, Örebro, Sweden.
    Kruse, Robert
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital, Örebro, Sweden.
    Fransén, Karin
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Wegman, Pia
    Linköping University Hospital, Linköping, Sweden.
    Repsilber, Dirk
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Montgomery, Scott
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden; Department of Epidemiology and Public Health, University College London, UK .
    Sollie, Tomas
    Örebro University Hospital, Örebro, Sweden.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    Gene expression profiles in breast tumors from tamoxifen treated patients with and without distant recurrenceManuskript (preprint) (Annet vitenskapelig)
  • 130.
    Göthlin Eremo, Anna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tina, Elisabet
    Örebro University Hospital.
    Wegman, Pia
    Linköping University Hospital.
    Stål, Olle
    Linköping University.
    Fransén, Karin
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Fornander, Tommy
    Karolinska University Hospital, Karolinska Institute; Regional Cancer Center Stockholm-Gotland,.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    HER4 tumor expression in breast cancer patients randomized to treatment with or without tamoxifenManuskript (preprint) (Annet (populærvitenskap, debatt, mm))
  • 131.
    Göthlin Eremo, Anna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tina, Elisabet
    Region Örebro län. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden.
    Wegman, Pia
    Department of Clinical Genetics, University Hospital, Linköping, Sweden.
    Stål, Olle
    Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
    Fransén, Karin
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Fornander, Tommy
    Department of Oncology, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden; Regional Cancer Center Stockholm-Gotland, Stockholm, Sweden.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    HER4 tumor expression in breast cancer patients randomized to treatment with or without tamoxifen2015Inngår i: International Journal of Oncology, ISSN 1019-6439, Vol. 47, nr 4, s. 1311-1320Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The human epidermal growth factor receptor (HER) 4 is a relative of HER2 and has been associated to endocrine breast cancer and prediction of tamoxifen response. In addition to PI3K/Akt and MAPK pathway activation, ligand binding to HER4 triggers proteolytic cleavage and release of an intracellular receptor domain (4ICD) with signaling properties. The aim of the present study was to analyze HER4 protein expression and intracellular localization in breast cancer tissue from patients randomized to treatment with or without adjuvant tamoxifen. To investigate HER4 expression and localization in response to estradiol (E2) and 4-hydroxytamoxifen (4-OHT) exposure, we also performed in vitro studies. Cytoplasmic, nuclear and membrane expression of HER4 protein was evaluated by immunohistochemical staining in tumor tissue from 912 breast cancer patients. Three different breast epithelia cancer cell lines were exposed to E2 and 4-OHT and mRNA expression was analyzed using qPCR. Further, nuclear and cytoplasmic proteins were separated and analyzed with western blotting. We found an association between nuclear HER4 protein expression and ER-positivity (P=0.004). Furthermore, significant association was found between cytoplasmic HER4 and ER-negativity (P<0.0005), PgR-negativity (P<0.0005), tumor size >20 mm (P=0.001) and HER2-negativity (P=0.008). However, no overall significance of HER4 on recurrence-free survival was found. After E2 exposure, HER4 mRNA and protein expression had decreased in two cell lines in vitro yet no changes in nuclear or cytoplasmic protein fractions were seen. In conclusion, nuclear HER4 seem to be co-located with ER, however, we did not find support for overall HER4 expression in independently predicting response of tamoxifen treatment. The possible influence of separate isoforms was not tested and future studies may further evaluate HER4 significance.

  • 132.
    Götlind, Y. Y.
    et al.
    Department of Microbiology and Immunology, Institute of Biomedicine and MIVAC, Sahlgrenska Academy, Göteborg, Sweden.
    Fritsch Fredin, M.
    Department of Bioscience, AstraZeneca, Mölndal, Sweden.
    Kumawat, Ashok Kumar
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Clinical Medicine.
    Strid, H.
    Department of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Willén, R.
    Department of Pathology and Cytology, Uppsala University Hospital, Uppsala, Sweden.
    Rangel, Ignacio
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Clinical Medicine.
    Bland, P. W.
    Department of Microbiology and Immunology, Institute of Biomedicine and MIVAC, Sahlgrenska Academy, Göteborg, Sweden.
    Hultgren Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning. Department of Clinical Medicine.
    Interplay between Th1 and Th17 effector T cell pathways in the pathogenesis of spontaneous colitis and colon cancer in the Gai2-deficient mouse2013Inngår i: International Immunology, ISSN 0953-8178, E-ISSN 1460-2377, Vol. 25, nr 1, s. 35-44Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Gαi2-deficient mice spontaneously develop colitis. Using xMAP technology and RT-PCR, we investigated cytokine/chemokine profiles during histologically defined phases of disease: (i) no/mild, (ii) moderate, (iii) severe colitis without dysplasia/cancer and (iv) severe colitis with dysplasia/cancer, compared with age-matched wild-type (WT) littermates. Colonic dysplasia was observed in 4/11 mice and cancer in 1/11 mice with severe colitis. The histology correlated with progressive increases in colon weight/cm and spleen weight, and decreased thymus weight, all more advanced in mice with dysplasia/cancer. IL-1β, IL-6, IL-12p40, IL-17, TNF-α, CCL2 and CXCL1 protein levels in colons, but not small intestines increased with colitis progression and were significantly increased in mice with moderate and severe colitis compared with WT mice, irrespective of the absence/presence of dysplasia/cancer. CCL5 did not change during colitis progression. Colonic IL-17 transcription increased 40- to 70-fold in all stages of colitis, whereas IFN-γ mRNA was gradually up-regulated 12- to 55-fold with colitis progression, and further to 62-fold in mice with dysplasia/cancer. IL-27 mRNA increased 4- to 15-fold during the course of colitis, and colonic IL-21 transcription increased 3-fold in mice with severe colitis, both irrespective of the absence/presence of dysplasia/cancer. FoxP3 transcription was significantly enhanced (3.5-fold) in mice with moderate and severe colitis, but not in mice with dysplasia/cancer, compared with WT mice. Constrained correspondence analysis demonstrated an association between increased protein levels of TNF-α, CCL2, IL-1β, IL-6 and CXCL1 and dysplasia/cancer. In conclusion, colonic responses are dominated by a mixed T(h)1/T(h)17 phenotype, with increasing T(h)1 cytokine transcription with progression of colitis in Gαi2(-/-) mice.

  • 133.
    Günaltay, Sezin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Nyhlin, Nils
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Kumawat, Ashok Kumar
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Hultgren, Olof
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Microbiology and Immunology, Örebro University Hospital, Örebro, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Differential expression of interleukin-1/Toll-like receptor signaling regulators in microscopic and ulcerative colitis2014Inngår i: World Journal of Gastroenterology, ISSN 1007-9327, E-ISSN 2219-2840, Vol. 20, nr 34, s. 12249-12259Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    AIM: To investigate Toll-like receptor (TLR) signaling regulators in microscopic and ulcerative colitis patients.

    METHODS: Total RNA and microRNA were isolated from fresh frozen colonic biopsies of non-inflamed controls and patients with active or in-remission collagenous colitis (CC), lymphocytic colitis (LC), or ulcerative colitis (UC). We compared expressions of interleukin-1 receptor-associated kinase (IRAK)-2, IRAK-M, interleukin (IL)-37, microRNA (miR)-146a, miR-155, and miR-21 using quantitative real time reverse transcription polymerase chain reaction.

    RESULTS: IRAK-M expression was increased in LC patients with active disease in histopathological remission (LC-HR; P = 0.02) and UC patients (P = 0.01), but no differences in IRAK-2 expression were detected compared to controls. miR-146a, -155 and -21 expressions were increased in LC-HR (P = 0.04, 0.07, and 0.004) and UC (P = 0.02, 0.04 and 0.03) patients. miR-146a and miR-21 expressions were significantly enhanced in UC patients compared to UC remission (UC-R; P = 0.01 and 0.04). Likewise, active CC patients showed significantly increased expression of miR-155 (P = 0.003) and miR-21 (P = 0.006). IL-37 expression was decreased in both CC (P = 0.03) and LC (P = 0.04) patients with a similar trend in UC patients but not statistically significant, whilst it was increased in UC-R patients compared to controls (P = 0.02) and active UC (P = 0.001).

    CONCLUSION: The identification of differentially expressed miRNAs, IL-37, and IRAK-M suggests different pathophysiologic mechanisms in various disease stages in LC, CC, and UC. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

  • 134.
    Halfvarson, Jonas
    et al.
    Örebro universitet, Institutionen för läkarutbildning.
    Jess, Tine
    Bodin, Lennart
    Örebro universitet, Handelshögskolan vid Örebro Universitet.
    Järnerot, Gunnar
    Munkholm, Pia
    Binder, Vibeke
    Tysk, Curt
    Örebro universitet, Hälsoakademin.
    Longitudinal concordance for clinical characteristics in a Swedish-Danish twin population with inflammatory bowel disease2007Inngår i: Inflammatory Bowel Diseases, ISSN 1078-0998, E-ISSN 1536-4844, Vol. 13, nr 12, s. 1536-1544Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    BACKGROUND: The genetic influence on disease course in inflammatory bowel disease (IBD) remains unknown. We therefore aimed to study longitudinal concordance for clinical characteristics and longitudinal stability using the Montreal Classification in an IBD twin population. METHODS: A total of 158 twins with ulcerative colitis (UC) (18 belonging to 9 concordant monozygotic pairs) and 141 twins with Crohn's disease (CD) (34 belonging to 17 concordant monozygotic pairs) were enrolled. Medical notes were scrutinized for clinical characteristics at diagnosis and after 10 years. Using the binominal distribution, we tested the hypothesis that clinical characteristics were independent within individuals in disease concordant monozygotic pairs. RESULTS: In CD, location was identical in 11/17 monozygotic concordant pairs at diagnosis (P = 0.008) and in 11/16 pairs after 10 years (P = 0.02). Behavior at diagnosis was identical in 13/17 pairs (P = 0.03) and in 11/16 pairs after 10 years (P = 0.01). Monozygotic UC twins were concordant (within 5 years) for age at diagnosis (6/9 pairs; P < 0.001) and symptomatic onset (4/9 pairs; P = 0.02) but not for extent of disease at diagnosis or after 10 years. The Montreal Classification did not demonstrate longitudinal stability, either regarding location or behavior of CD or extent of UC. CONCLUSIONS: The high phenotypic concordance, both at diagnosis and longitudinally, in monozygotic twins with CD supports a genetic influence not only on disease occurrence but also on disease course. This contrasts with UC, where the genetic impact appears less. Montreal Classification characteristics changed over time and should be used cautiously.

  • 135.
    Hamad, Tarza
    Örebro universitet, Institutionen för läkarutbildning.
    Antibiotic susceptibility of Staphylococcus epidermidis isolated from prosthetic joint infections, with special focus on doxycycline2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 136.
    Hamad, Tarza
    et al.
    Department of Laboratory Medicine, Clinical Microbiology, Örebro University, Örebro, Sweden; Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Hellmark, Bengt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Clinical Microbiology, Örebro University, Örebro, Sweden.
    Nilsdotter-Augustinsson, Åsa
    Department of Infectious Diseases, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Clinical Microbiology, Örebro University, Örebro, Sweden.
    Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections, with focus on doxycycline2015Inngår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 123, nr 12, s. 1055-1060Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In recent years, coagulase-negative staphylococci such as Staphylococcus epidermidis have gained importance as nosocomial pathogens, especially in immunocompromised patients and prosthetic joint infections (PJIs). These infections are often long lasting and difficult to treat due to the production of bacterial biofilm and the transformation of the bacteria into a stationary growth phase. Rifampicin is able to penetrate the biofilm, but to reduce the risk of development of rifampicin resistance it should be used in combination with an additional antibiotic. In this study we used Etest to investigate the antimicrobial susceptibility of 134 clinical isolates of S.epidermidis obtained from PJIs to six oral antibiotics: doxycycline, rifampicin, linezolid, fusidic acid, clindamycin, and ciprofloxacin. We also performed synergy testing on doxycycline in combination with each of the remaining antibiotics. Ninety-three (69%) of the 134 isolates were susceptible to doxycycline, 94/134 (70%) to rifampicin, 56/134 (42%) to clindamycin, 25/134 (19%) to ciprofloxacin, 81/134 (60%) to fusidic acid, and 100% to linezolid. Thirty-two (80%) of the 40 isolates not fully susceptible to rifampicin were susceptible to doxycycline. Doxycycline in combination with each of the other investigated antibiotics exerted an additive effect on nearly half of the isolates, with the exception of clindamycin, which displayed an even higher percentage of additive effect (69%). To conclude, as the majority of the S.epidermidis isolates were susceptible to doxycycline, this antimicrobial agent may provide a potential alternative for combination therapy together with rifampicin.

  • 137.
    Hamsten, C.
    et al.
    Department of Medicine Solna, Clinical Immunology and Allergy Unit, Karolinska Institutet and University Hospital Solna, Stockholm, Sweden; Center for Inflammatory Diseases, Karolinska Institutet, Stockholm, Sweden.
    Starkhammar, M.
    Department of Internal Medicine, Södersjukhuset, Stockholm, Sweden.
    Tran, T. A.
    Department of Medicine Solna, Clinical Immunology and Allergy Unit, Karolinska Institutet and University Hospital Solna, Stockholm, Sweden.
    Johansson, Magnus
    Örebro universitet, Institutionen för läkarutbildning. School of Life Sciences, Södertörns University, Stockholm, Sweden.
    Bengtsson, U.
    Department of Internal Medicine, Södersjukhuset, Stockholm, Sweden.
    Ahlén, G.
    Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet and University Hospital Huddinge, Stockholm, Sweden.
    Sällberg, M.
    Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet and University Hospital Huddinge, Stockholm, Sweden.
    Grönlund, H.
    Department of Medicine Solna, Clinical Immunology and Allergy Unit, Karolinska Institutet and University Hospital Solna, Stockholm, Sweden.
    van Hage, M.
    Department of Medicine Solna, Clinical Immunology and Allergy Unit, Karolinska Institutet and University Hospital Solna, Stockholm, Sweden.
    Identification of galactose-α-1,3-galactose in the gastrointestinal tract of the tick Ixodes ricinus; possible relationship with red meat allergy2013Inngår i: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 68, nr 4, s. 549-552Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Patients with IgE antibodies against the carbohydrate epitope galactose-α-1,3-galactose (α-Gal) have reported severe allergic reactions after consumption of red meat. Investigations have revealed associations between IgE to α-Gal and tick bites. We provide the first direct evidence that α-Gal is present within ticks thus potentially explaining the relationship between tick exposure and sensitization to α-Gal, with development of red meat allergy as a secondary phenomena. Serum from Swedish patients with delayed severe reactions to red meat was included in the study. A dose-dependent inhibition of IgE responses to α-Gal by the tick Ixodes ricinus is demonstrated. Furthermore, using cryostat-cut sections of I. ricinus, we show that both a monoclonal and a polyclonal antibody against α-Gal stains the gastrointestinal tract of the tick. The same pattern is seen when staining with patient sera IgE positive to α-Gal. These results confirm that the α-Gal epitope is present in I. ricinus and imply host exposure to α-Gal during a tick bite. This provides further evidence that tick bites are associated with IgE responses to α-Gal and red meat allergy.

  • 138.
    Hansson Bergén, Mattias
    Örebro universitet, Institutionen för läkarutbildning.
    ADENOSINTRIFOSFAT (ATP) SOM EXTRACELLULÄR SIGNALMOLEKYL I KOLONCANCERCELLINJEN HT-292014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 139.
    Hansson, Fanny
    Örebro universitet, Institutionen för läkarutbildning.
    Thymectomy in patients with myasthenia gravis – a retrospective cohort study2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 140.
    Hanås, Ragnar
    et al.
    Uddevalla Hospital, Uddevalla, Sweden.
    Eriksson, Miriam
    Särnblad, Stefan
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Örebro, Sweden.
    Diabetes - ketoacidos, hyperglukemi och hypoglukemi2010Inngår i: Akut Pediatrik / [ed] Svante Norgren, Jonas F. Ludvigsson, Mikael Norman, Stockholm: Liber, 2010, 7:e, s. 253-269Kapittel i bok, del av antologi (Annet vitenskapelig)
  • 141.
    Hartman, Linnea
    Örebro universitet, Institutionen för läkarutbildning.
    The sporicidal effect of Chlorine dioxide against Clostridium Difficile spores with and without presence of organic material2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 142.
    Hartwig, Markus
    Örebro universitet, Institutionen för läkarutbildning.
    Skillnader i postoperativsmärtupplevelse mellan patientersom sövts med TIVA respektive inhalationsanestesi.2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 143.
    Heap, Graham A.
    et al.
    BD Pharmacogenetics, Royal Devon and Exeter Foundation Trust, Exeter, UK; Precision Medicine Exeter, University of Exeter, Exeter, UK .
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning. Department of Gastroenterology, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Ahmad, Tariq
    BD Pharmacogenetics, Royal Devon and Exeter Foundation Trust, Exeter, UK; Precision Medicine Exeter, University of Exeter, Exeter, UK .
    Clinical Features and HLA Association of 5-Aminosalicylate (5-ASA)-induced Nephrotoxicity in Inflammatory Bowel Disease2016Inngår i: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 10, nr 2, s. 149-158Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and Aims: Nephrotoxicity is a rare idiosyncratic reaction to 5-aminosalicylate (5-ASA) therapies. The aims of this study were to describe the clinical features of this complication and identify clinically useful genetic markers so that these drugs can be avoided or so that monitoring can be intensified in high-risk patients.

    Methods: Inflammatory bowel disease patients were recruited from 89 sites around the world. Inclusion criteria included normal renal function prior to commencing 5-ASA, >= 50% rise in creatinine any time after starting 5-ASA, and physician opinion implicating 5-ASA strong enough to justify drug withdrawal. An adjudication panel identified definite and probable cases from structured case report forms. A genome-wide association study was then undertaken with these cases and 4109 disease controls.

    Results: After adjudication, 151 cases of 5-ASA-induced nephrotoxicity were identified. Sixty-eight percent of cases were males, with nephrotoxicity occurring at a median age of 39.4 years (range 6-79 years). The median time for development of renal injury after commencing 5-ASA was 3.0 years (95% confidence interval [CI] 2.3-3.7). Only 30% of cases recovered completely after drug withdrawal, with 15 patients requiring permanent renal replacement therapy. A genome-wide association study identified a suggestive association in the HLA region (p = 1 x 10(-7)) with 5-ASA-induced nephrotoxicity. A sub-group analysis of patients who had a renal biopsy demonstrating interstitial nephritis (n = 55) significantly strengthened this association (p = 4 x 10(-9), odds ratio 3.1).

    Conclusions: This is the largest and most detailed study of 5-ASA-induced nephrotoxicity to date. It highlights the morbidity associated with this condition and identifies for the first time a significant genetic predisposition to drug-induced renal injury.

  • 144. Heap, Graham A.
    et al.
    Singh, Abhey
    Bewshea, Claire M.
    Weedon, Michael
    Dubois, Patrick
    Andrews, Jane M.
    Annese, Vito
    Bampton, Peter A.
    Bell, Sally
    Cole, Andy
    Connor, Susan J.
    Creed, Tom
    D'Amato, Mauro
    Fedorak, Richard N.
    Florin, Timothy H.
    Gaya, Daniel R.
    Greig, Emma
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning.
    Irving, Peter M.
    Karban, Amir
    Lawrance, Ian C.
    Lev-Tzion, Raffi
    Lindsay, James O.
    Mawsley, Joel
    Mazher, Zia
    Orchard, Timothy R.
    Radford-Smith, Graham L.
    Reffitt, David
    Silverberg, Mark S.
    Sturniolo, Giacomo C.
    Tsianos, Epameinondas V.
    Walsh, Alissa
    Zeissig, Sebastian
    Holden, Arthur L.
    Ahmad, Tariq
    Thiopurine induced pancreatitis in inflammatory bowel disease: clinical features and genetic determinants2014Inngår i: Gastroenterology, ISSN 0016-5085, E-ISSN 1528-0012, Vol. 146, nr 5, Supplement 1, s. S2-S2Artikkel i tidsskrift (Fagfellevurdert)
  • 145.
    Heap, Graham A.
    et al.
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England; Precis Med, Univ Exeter, Exeter, England.
    Weedon, Michael N.
    Precision medicine, Exeter Medical School, University of Exeter, Exeter, England.
    Bewshea, Claire M.
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England; Precision Med Exeter, Univ Exeter, Exeter, England.
    Singh, Abhey
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England.
    Chen, Mian
    Oxford Transplant Ctr, Royal Devon & Exeter Hosp, Oxford, England.
    Satchwel, Jack B.
    Oxford Transplant Ctr, Oxford Univ Hosp Natl Hlth Serv NHS Trust, Oxford, England.
    Vivian, Julian P.
    Sch Biomed Sci, Dept Biochem & Mol Biol, Monash Univ, Clayton Vic, Australia.
    So, Kenji
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England.
    Dubois, Patrick C.
    Dept Gastroenterol, Kings Coll Hosp, London, England.
    Andrews, Jane M.
    Dept Gastroenterol, IBD Serv, Adelaide SA, Australia; Royal Adelaide Hosp, Univ Adelaide, Adelaide SA, Australia.
    Annese, Vito
    Div Gastroenterol, Azienda Osped, Univ Careggi, Florence, Italy.
    Bampton, Peter
    Flinders Med Ctr, Flinders Univ South Australia, Adelaide SA, Australia.
    Barnardo, Martin
    Oxford Transplant Ctr, Oxford Univ Hosp Natl Hlth Serv NHS Trust, Oxford, England.
    Bell, Sally
    Dept Gastroenterol, St Vincent Hosp, Fitzroy Vic, Australia.
    Cole, Andy
    Royal Derby Hosp, Derby, England.
    Connor, Susan J.
    Dept Gastroenterol & Hepatol, Liverpool Hosp, Sydney NSW, Australia.
    Creed, Tom
    Joint Clin Res Unit, Univ Hosp Bristol NHS Fdn Trust, Bristol, England.
    Cummings, Fraser R.
    Dept Gastroenterol, Univ Hosp Southampton NHS Fdn Trust, Southampton, England.
    D'Amato, Mauro
    Dept Biosci & Nutr, Karolinska Inst, Stockholm, Sweden.
    Daneshmend, Tawfique K.
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England.
    Fedorak, Richard N.
    Div Gastroenterol, Univ Alberta, Edmonton AB, Canada.
    Florin, Timothy H.
    Sch Med, Univ Queensland, South Brisbane Qld, Australia.
    Gaya, Daniel R.
    Gastroenterol Unit, Glasgow Royal Infirm, Glasgow, UK.
    Greig, Emma
    Dept Gastroenterol, Taunton & Somerset NHS Fdn Trust, Taunton, England.
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Hart, Alisa
    Dept Med, St Marks Hosp, London, England; Acad Inst, North West London Hosp NHS Trust, London, England.
    Irving, Peter M.
    Dept Gastroenterol, Guys & St Thomas NHS Fdn Trust, London, England.
    Jones, Gareth
    Dept Gastroenterol, Western Gen Hosp, Edinburgh, UK.
    Karban, Amir
    Dept Gastroenterol, Rambam Med Ctr, Haifa, Israel.
    Lawrance, Ian C.
    Ctr Inflammatory Bowel Dis, Fremantle Hosp, Univ Western Australia, Fremantle WA, Australia.
    Lee, James C.
    Dept Gastroenterol, Cambridge Univ Hosp NHS Trust, Cambridge, England.
    Lees, Charlie
    Dept Gastroenterol, Western Gen Hosp, Edinburgh, UK.
    Lev-Tzion, Raffi
    Paediatr Gastroenterol & Nutr Unit, Shaare Zedek Med Ctr, Jerusalem, Israel.
    Lindsay, James
    Dept Gastroenterol, Barts & London NHS Trust, London, England.
    Mansfield, John
    Dept Gastroenterol, Newcastle Univ Hosp NHS Trust, Newcastle NSW, Australia.
    Mawdsley, Joel
    Dept Gastroenterol, West Middlesex Univ Hosp NHS Trust, Isleworth, England.
    Mazhar, Zia
    Dept Gastroenterol, Basildon & Thurrock Hosp NHS Trust, Basildon, England.
    Parkes, Miles
    Dept Gastroenterol, Cambridge Univ Hosp NHS Trust, Cambridge, England.
    Parnell, Kirstie
    Precision Med Exeter, Univ Exeter, Exeter, England.
    Orchard, Timothy R.
    Dept Med, Imperial Coll Healthcare NHS, London, England.
    Radford-Smith, Graham
    Dept Gastroenterol, Royal Brisbane & Womens Hosp, Brisbane Qld, Australia; IBD Grp, Queensland Inst Med Res, Brisbane Qld, Australia; Sch Med, Univ Queensland, Brisbane Qld, Australia.
    Russell, Richard K.
    Dept Paediat Gastroenterol, Yorkhill Hosp, Glasgow, UK.
    Reffitt, David
    Dept Gastroenterol, Lewisham & Greenwich NHS Trust, London, England.
    Satsangi, Jack
    Dept Gastroenterol, Western Gen Hosp, Edinburgh, UK.
    Silverberg, Mark S.
    Zane Cohen Ctr Digest Dis, Inflammatory Bowel Dis Grp, Mt Sinai Hosp, Toronto ON, Canada.
    Sturniolo, Giacomo C.
    Univ Padua, Padua, Italy.
    Tremelling, Mark
    Dept Gastroenterol, Norfolk & Norwich Hosp NHS Trust, Norwich, England.
    Tsianos, Epameinondas V.
    Fac Med, Div Internal Med 1, Univ Ioannina, Ioannina, Greece; Fac Med, Div Gastroenterol, Univ Ioannina, Ioannina, Greece.
    van Heel, David A.
    Barts & London Sch Med & Dent, Blizard Inst, Queen Mary Univ, London, England.
    Walsh, Alissa
    Dept Gastroenterol, St Vincents Hosp, Sydney NSW, Australia.
    Watermeyer, Gill
    Gastrointestinal Clin, Groote Schuur Hosp, Cape Town, South Africa.
    Weersma, Rinse K.
    Dept Gastroenterol & Hepatol, Univ Med Ctr, Groningen, Netherlands; Univ Groningen, Groningen, Netherlands.
    Zeissig, Sebastian
    Dept Internal Med, Univ Med Ctr Schleswig Holstein, Kiel, Germany.
    Rossjohn, Jamie
    Sch Biomed Sci, Dept Biochem & Mol Biol, Monash Univ, Clayton Vic, Australia.
    Holden, Arthur L.
    Int Serious Adverse Events Consortium, Chicago IL, USA.
    Ahmad, Tariq
    IBD Pharmacogenet, Royal Devon & Exeter Hosp, Exeter, England; Precision Med, Univ Exeter, Exeter, England.
    HLA-DQA1-HLA-DRB1 variants confer susceptibility to pancreatitis induced by thiopurine immunosuppressants2014Inngår i: Nature Genetics, ISSN 1061-4036, E-ISSN 1546-1718, Vol. 46, nr 10, s. 1131-1134Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Pancreatitis occurs in approximately 4% of patients treated with the thiopurines azathioprine or mercaptopurine. Its development is unpredictable and almost always leads to drug withdrawal. We identified patients with inflammatory bowel disease (IBD) who had developed pancreatitis within 3 months of starting these drugs from 168 sites around the world. After detailed case adjudication, we performed a genome-wide association study on 172 cases and 2,035 controls with IBD. We identified strong evidence of association within the class II HLA region, with the most significant association identified at rs2647087 (odds ratio 2.59, 95% confidence interval 2.07-3.26, P = 2 x 10(-16)). We replicated these findings in an independent set of 78 cases and 472 controls with IBD matched for drug exposure. Fine mapping of the H LA region identified association with the HLA-DQA1*02:01-HLA-DRB1*07:01 haplotype. Patients heterozygous at rs2647087 have a 9% risk of developing pancreatitis after administration of a thiopurine, whereas homozygotes have a 17% risk.

  • 146.
    Hedbrant, Alexander
    et al.
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Erlandsson, Ann
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Delbro, Dick
    Örebro universitet, Institutionen för läkarutbildning.
    Wijkander, Jonny
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Conditioned media from human macrophages of M1 phenotype attenuate the cytotoxic effect of 5-fluorouracil on the HT-29 colon cancer cell line2015Inngår i: International Journal of Oncology, ISSN 1019-6439, Vol. 46, nr 1, s. 37-46Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Resistance of tumor cells to chemotherapy, such as 5-fluorouracil (5-FU), is an obstacle for successful treatment of cancer. As a follow-up of a previous study we have investigated the effect of conditioned media (CM) from macrophages of M1 or M2 phenotypes on 5-FU cytotoxicity on the colon cancer cell lines HT-29 and CACO-2. HT-29 cells, but not CACO-2 cells, having been treated with a combination of M1 CM and 5-FU recovered their cell growth to a much larger extent compared to cells having been treated with 5-FU alone when further cultured for 7 days in fresh media. M1 CM treatment of HT-29, but not CACO-2 cells, induced cell cycle arrest in the G(0)/G(1) and G(2)/M phases. 5-FU treatment induced accumulation of cells in S-phase in both HT-29 and CACO-2 cells. This accumulation of cells in S-phase was attenuated by combined M1 CM and 5-FU treatment in HT-29 cells, but not in CACO-2 cells. The mRNA expression of cell cycle regulatory proteins and 5-FU metabolic enzymes were analyzed in an attempt to find possible mechanisms for the M1 CM induced attenuation of 5-FU cytotoxicity in HT-29. Thymidylate synthetase (TS) and thymidine phosphorylase (TP) were found to be substantially downregulated and upregulated, respectively, in HT-29 cells treated with M1 CM, making them unlikely as mediators of reduced 5-FU cytotoxicity. Among cell cycle regulating proteins, p21 was induced in HT-29 cells, but not in CACO-2 cells, in response to M1 CM treatment. However, small interfering RNA (siRNA) knockdown of p21 had no effect on the M1 CM induced cell cycle arrest seen in HT-29 and neither did it change the growth recovery after combined treatment of HT-29 cells with M1 CM and 5-FU. In conclusion, treatment of HT-29 cells with M1 CM reduces the cytotoxic effect of 5-FU and this is mediated by a M1 CM induced cell cycle arrest in the G(0)/G(1) and G(2)/M phases. So far, we lack an explanation why this action is absent in the CACO-2 cells. The current findings may be important for optimization of chemotherapy in colon cancer.

  • 147.
    Hedbrant, Alexander
    et al.
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Wijkander, Jonny
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Seidal, Tomas
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Delbro, Dick
    Örebro universitet, Institutionen för läkarutbildning.
    Erlandsson, Ann
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Macrophages of M1 phenotype have properties that influence lung cancer cell progression2015Inngår i: Tumor Biology, ISSN 1010-4283, E-ISSN 1423-0380, Vol. 36, nr 11, s. 8715-8725Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Stromal macrophages of different phenotypes can contribute to the expression of proteins that affects metastasis such as urokinase-type plasminogen activator (uPA), its receptor uPAR, and plasminogen activator inhibitor-1 (PAI-1), but knowledge of how essential their contribution is in comparison to the cancer cells in small cell lung cancer (SCLC) and lung squamous cell carcinoma (SCC) is lacking. The expression of uPA, uPAR, and PAI-1 and of the matrix metalloproteinases (MMP)-2 and MMP-9 were studied in human macrophages of M1 and M2 phenotype and compared to a lung SCC (NCI-H520) and a SCLC (NCI-H69) cell line. Effects of treatment with conditioned media (CM) from M1 and M2 macrophages on the expression of these genes in H520 and H69 cells as well as effects on the cell growth were investigated. In addition, data on the stromal macrophages immunoreactivity of uPAR, MMP-2, and MMP-9 in a few SCC and SCLC biopsies was included. uPAR, MMP-2, and MMP-9 were confirmed in stromal cells including macrophages in the SCC and SCLC biopsies. In vitro, both macrophage phenotypes expressed considerably higher mRNA levels of uPA, uPAR, PAI-1, and MMP-9 compared to the cancer cell lines, and regarding uPAR, the highest level was found in the M1 macrophage phenotype. Furthermore, M1 CM treatment not only induced an upregulation of PAI-1 in both H520 and H69 cells but also inhibited cell growth in both cell lines, giving M1 macrophages both tumor-promoting and tumor-killing potential.

  • 148.
    Hedin, Marie
    Örebro universitet, Institutionen för läkarutbildning.
    Recurrence after pneumothorax surgery Author:2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 149.
    Hellmark, Bengt
    et al.
    Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Unemo, Magnus
    Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Nilsdotter-Augustinsson, Åsa
    Division of Infectious Diseases, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, University of Linköping, Linköping, Sweden.
    Comparison of Staphylococcus epidermidis isolated from prosthetic joint infections and commensal isolates in regard to antibiotic susceptibility, agr type, biofilm production, and epidemiology2013Inngår i: International Journal of Medical Microbiology, ISSN 1438-4221, E-ISSN 1618-0607, Vol. 303, nr 1, s. 32-39Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Staphylococcus epidermidis is the predominant bacterial species in the normal flora of the human skin and superficial mucosal membranes. However, it has also emerged as the most important pathogen in infections related to foreign-body materials, such as prosthetic joints and heart valves. The aims of this study were to characterise S. epidermidis isolated from prosthetic joint infections (PJI; n = 61) and commensal isolates from healthy individuals (n = 24) in regard to antimicrobial sensitivity, agr type, hid gene presence, biofllm production including presence of ica and aap genes involved in the biofilm formation process and epidemiology using both phenotypic (the PhenePlate-system) and genotypic [multilocus sequence typing (MLST)] methods. Among the PJI isolates, the majority (67%) were multidrug-resistant. Two major clusters of PJI isolates could be identified; 44% belonged to MLST sequence type (ST) 2, all but one were of agr type 1, and 31% were assigned ST215 and were of agr type 3. Of the commensal isolates, only one isolate was multidrug-resistant, and they were more molecular epidemiologically diverse with mainly MLST singletons and a maximum of 3 isolates assigned to the identical ST. Biofilm production was detected in 41% of the PJI isolates and 58% of the commensal isolates, with the aap gene (95%) more frequently detected than the ica genes (62%) in the biofilm-positive isolates. In conclusion, S. epidermidis isolated from PJIs and commensal isolates differed regarding antimicrobial sensitivity and molecular epidemiological typing using MLST, but not substantially in the distribution of agr types, biofilm production, or the presence of ica and aap genes.

  • 150.
    Helmrot, Kristina
    Örebro universitet, Institutionen för läkarutbildning.
    Survey of patient influx to emergency department at Örebro University Hospital: Survey of patient influx to emergency department at Örebro University Hospital2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
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