oru.sePublikationer
Ändra sökning
Avgränsa sökresultatet
2345678 201 - 250 av 464
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Träffar per sida
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
Markera
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 201.
    Klarström-Engström, Kristin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Khalaf, Hazem
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kälvegren, H.
    Department of Clinical Pathology and Clinical Genetics, Linköping University Hospital, Linköping, Sweden.
    Bengtsson, Torbjörn
    Örebro universitet, Institutionen för läkarutbildning.
    The role of Porphyromonas gingivalis gingipains in platelet activation and innate immune modulation2015Ingår i: Molecular Oral Microbiology, ISSN 2041-1006, E-ISSN 2041-1014, Vol. 30, nr 1, s. 62-73Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Platelets are considered to have important functions in inflammatory processes and as actors in the innate immunity. Several studies have shown associations between cardiovascular disease and periodontitis, where the oral anaerobic pathogen Porphyromonas gingivalis has a prominent role in modulating the immune response. Porphyromonas gingivalis has been found in atherosclerotic plaques, indicating spreading of the pathogen via the circulation, with an ability to interact with and activate platelets via e.g. Toll-like receptors (TLR) and protease-activated receptors. We aimed to evaluate how the cysteine proteases, gingipains, of P.gingivalis affect platelets in terms of activation and chemokine secretion, and to further investigate the mechanisms of platelet-bacteria interaction. This study shows that primary features of platelet activation, i.e. changes in intracellular free calcium and aggregation, are affected by P.gingivalis and that arg-gingipains are of great importance for the ability of the bacterium to activate platelets. The P.gingivalis induced a release of the chemokine RANTES, however, to a much lower extent compared with the TLR2/1-agonist Pam(3)CSK(4), which evoked a time-dependent release of the chemokine. Interestingly, the TLR2/1-evoked response was abolished by a following addition of viable P.gingivalis wild-types and gingipain mutants, showing that both Rgp and Kgp cleave the secreted chemokine. We also demonstrate that Pam(3)CSK(4)-stimulated platelets release migration inhibitory factor and plasminogen activator inhibitor-1, and that also these responses were antagonized by P.gingivalis. These results supports immune-modulatory activities of P.gingivalis and further clarify platelets as active players in innate immunity and in sensing bacterial infections, and as target cells in inflammatory reactions induced by P.gingivalis infection.

  • 202.
    Klarström-Engström, Kristin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Magnusson, A.
    Demirel, I.
    Lönn, J.
    Starkhammar Johansson, C.
    Kälvegren, Hanna
    Department of Clinical Pathology and Clinical Genetics, Linköping University Hospital, Linköping, Sweden; Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden .
    Bengtsson, Torbjörn
    Örebro universitet, Institutionen för läkarutbildning.
    Porphyromonas gingivalis-induced lipid peroxidation: the role of platelets, gingipains and periodontal status.Manuskript (preprint) (Övrigt vetenskapligt)
  • 203.
    Klarström-Engström, Kristin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Skoglund, C.
    Linköping University, Linköping, Sweden.
    Kälvegren, Hanna
    Linköping University Hospital, Linköping, Sweden.
    Bengtsson, Torbjörn
    Örebro universitet, Institutionen för läkarutbildning.
    The role of platelets in inflammation at sites of infection: toll like receptor 2/1 mediated platelet adhesion on bacterial peptide-mimetic surfaces2012Ingår i: Cardiovascular Research, ISSN 0008-6363, E-ISSN 1755-3245, Vol. 93, s. S8-S8Artikel i tidskrift (Övrigt vetenskapligt)
  • 204.
    Kongstad, Isabella
    Örebro universitet, Institutionen för läkarutbildning.
    Evaluation of existing screening and outcome of pre-eclampsia in Örebro County2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 205.
    Koskinen, Masi
    Örebro universitet, Institutionen för läkarutbildning.
    Perioperative pregabalin in pain management after laparoscopic cholecystectomy: A quality of evidence assessment2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 206.
    Kosuta, Vezita
    Örebro universitet, Institutionen för läkarutbildning.
    How successful is treatment of ectopic pregnancy with methotrexate?: Gynecology Clinic at Örebro University hospital, between the years of 2013 and 2014 2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 207.
    Krauss, Wolfgang
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Gunnarsson, Martin
    Örebro universitet, Institutionen för läkarutbildning.
    Andersson, Torbjorn
    Örebro universitet, Institutionen för läkarutbildning.
    Thunberg, Per
    Department of Medical Physics, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Accuracy and reproducibility of a quantitative magnetic resonance imaging method for concurrent measurements of tissue relaxation times and proton density2015Ingår i: Magnetic Resonance Imaging, ISSN 0730-725X, E-ISSN 1873-5894, Vol. 33, nr 5, s. 584-591Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To evaluate the accuracy and reproducibility of a quantitative magnetic resonance (qMR) imaging method (QRAPMASTER) for simultaneous measurements of T1 and T2 relaxation times, and proton density (PD).

    Materials and Methods: Measurements of T1, T2, and PD with qMR were performed using phantoms with different relaxation times and concentrations of heavy water. Healthy volunteers were examined with different head coils. Regional measurements were performed in normal-appearing white and gray matter from the healthy control subjects, and in multiple sclerosis (MS) patients.

    Results: In phantom measurements, QRAPMASTER slightly underestimated T1, and T2 variations between repeated measurements were modest. PD was generally overestimated. The overall relative difference was 1.2 5.3% (T1), 6.6 1.9% (12), and 0.7 5.1% (PD). In healthy volunteers, there were no statistically significant differences of T1, T2 or PD using different head coils. Values of T1, T2, and PD obtained in healthy controls and MS patients were within reference ranges. However, significant differences were found in normal-appearing gray and white matter.

    Conclusion: QRAPMASTER can be considered a sufficiently accurate and reproducible method for use in clinical practice. Neuropathology in normal-appearing brain tissue may be revealed using this MR method, with putative implications for quantification of tissue damage in neurological diseases. (C) 2015 Elsevier Inc. All rights reserved.

  • 208.
    Kronqvist, Johanna
    Örebro universitet, Institutionen för läkarutbildning.
    A pilot study on the effect of Mindfulness Based StressReduction on emotion regulation2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 209.
    Kruse, Robert
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Clinical Research Centre (KFC), Örebro University Hospital, Örebro, Sweden.
    Demirel, Isak
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Säve, Susanne
    School of Natural Sciences, Linnaeus University, Kalmar, Sweden.
    Persson, Katarina
    Örebro universitet, Institutionen för läkarutbildning. Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    IL-8 and global gene expression analysis define a key role of ATP in renal epithelial cell responses induced by uropathogenic bacteria2014Ingår i: Purinergic Signalling Purinergic Signalling, ISSN 1573-9538, E-ISSN 1573-9546, Vol. 10, nr 3, s. 499-508Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The recent recognition of receptor-mediated ATP signalling as a pathway of epithelial pro-inflammatory cytokine release challenges the ubiquitous role of the TLR4 pathway during urinary tract infection. The aim of this study was to compare cellular responses of renal epithelial cells infected with uropathogenic Escherichia coli (UPEC) strain IA2 to stimulation with ATP-gamma-S. A498 cells were infected or stimulated in the presence or absence of apyrase, that degrades extracellular ATP, or after siRNA-mediated knockdown of ATP-responding P2Y(2) receptors. Cellular IL-8 release and global gene expression were analysed. Both IA2 and A498 cells per se released ATP, which increased during infection. IA2 and ATP-gamma-S caused a similar to 5-fold increase in cellular release of IL-8 and stimulations performed in the presence of apyrase or after siRNA knockdown of P2Y(2) receptors resulted in attenuation of IA2-mediated IL-8 release. Microarray results show that both IA2 and ATP-gamma-S induced marked changes in gene expression of renal cells. Thirty-six genes were in common between both stimuli, and many of these are key genes belonging to classical response pathways of bacterial infection. Functional analysis shows that 88 biological function-annotated cellular pathways were identical between IA2 and ATP-gamma-S stimuli. Results show that UPEC-induced release of IL-8 is dependent on P2Y(2) signalling and that cellular responses elicited by UPEC and ATP-gamma-S have many identical features. This indicates that renal epithelial responses elicited by bacteria could be mediated by bacteria- or host-derived ATP, thus defining a key role of ATP during infection.

  • 210.
    Kumakech, Edward
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Makerere University College of Health Sciences, Kampala, Uganda.
    Berggren, Vanja
    Medical Faculty, Lund University, Lund; Global Health, Karolinska Institute, Stockholm.
    Lillsunde-Larsson, Gabriella
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro.
    Kaliff, Malin
    Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University.
    Karlsson, Mats
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro.
    Musubika, Carol
    Makerere University College of Health Sciences, Kampala, Uganda.
    Kirimunda, Samuel
    Makerere University College of Health Sciences, Kampala, Uganda.
    Wabinga, Henry
    Makerere University College of Health Sciences, Kampala, Uganda.
    Andersson, Sören
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University.
    Prevalence, genotypes and risk factors for vaccine and non-vaccine types of Human Papillomavirus (HPV) infections among Bivalent HPV-16/18 vaccinated and non-vaccinated young women in Ibanda district Uganda: 5 year follow up studyManuskript (preprint) (Övrigt vetenskapligt)
  • 211.
    Kumawat, Ashok Kumar
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Elgbratt, Kristina
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro universitet, Hälsoakademin. Division of Gastroenterology, Department of Medicine, Örebro University Hospital, Region Örebro County, Örebro, sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Reduced T cell receptor excision circle levels in the colonic mucosa of microscopic colitis patients indicate local proliferation rather than homing of peripheral lymphocytes to the inflamed mucosa2013Ingår i: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141, artikel-id 408638Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Dysregulated T cell responses in the intestine may lead to chronic bowel inflammation such as collagenous colitis (CC) and lymphocytic colitis (LC), together known as microscopic colitis (MC). Having demonstrated increased local T cell responses in the intestinal mucosa of MC patients, we investigated the recent thymic emigrants by measuring T cell receptor excision circle (TREC) levels in the colonic biopsies from CC (n = 8), LC (n = 5), and CC or LC patients in histopathological remission (CC-HR, n = 3) (LC-HR, n = 6), non-inflamed diarrhoea patients (n = 17), and controls (n = 10) by real-time PCR. We observed lower median TREC levels in both CC and LC patients as well as in LC-HR patients compared to controls. In contrast to MC patients, non-inflamed diarrhoea patients presented with enhanced TREC levels compared to controls. None of the recorded differences did, however, reach statistical significance. A trend towards increased relative expression of CD3 was noted in all MC subgroups examined and reached statistical significance in LC patients compared to controls. In conclusion, reduced TRECs level in the colonic mucosa, together with our previously demonstrated enhanced expression of Ki67(+) T cells, suggests local expansion of resident T lymphocytes in the inflamed mucosa of MC patients.

  • 212.
    Kumawat, Ashok Kumar
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Nyhlin, Nils
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Wickbom, Anna
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Hultgren, Olof
    Region Örebro län. Department of Microbiology and Immunology, Örebro University Hospital, Örebro, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4(+) T Cells2014Ingår i: Mediators of Inflammation, ISSN 0962-9351, E-ISSN 1466-1861, artikel-id 879843Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Soluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut. We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation of T cells. Peripheral blood CD4(+) T cells from healthy donors were polyclonally activated in the presence of conditioned medium (CM) generated from denuded biopsies (DNB) or isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients compared to noninflamed controls, to determine proliferation and secretion of cytokines involved in T-cell differentiation. Compared to controls, we observed significantly increased production of the proinflammatory cytokines IFN-gamma, IL-17A, IL-6, and IL-1 beta and the anti-inflammatory cytokines IL-4 and IL-10 in the presence of CC-DNB-CM. The most pronounced effect of CC-LPMC-CM on peripheral CD4(+) T cells was a trend towards increased production of IL-17A and IL-10. A trend towards reduced inhibition of T-cell proliferation was noted in the presence of CC-DNB-CM. In conclusion, our in vitro model reveals implications of soluble factors from CC colonic mucosa on peripheral T cells, enhancing their production of both pro-and anti-inflammatory cytokines.

  • 213. Kumawat, Ashok Kumar
    et al.
    Strid, H.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bohr, J.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Patienter med mikroskopisk kolit har blandad Th1/Th17 samt CTL-associerad cytokinprofil2012Konferensbidrag (Övrigt vetenskapligt)
  • 214.
    Kumawat, Ashok Kumar
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Strid, Hilja
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Elgbratt, Kristina
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Dept. of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Dept. of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden.
    Hultgren Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Microscopic colitis patients have increased frequencies of Ki67+proliferating and CD45RO+ active/memory CD8+ and CD4+8mucosal T cells2013Ingår i: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 7, nr 9, s. 694-705Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Collagenous colitis (CC) and lymphocytic colitis (LC) are chronic inflammatory bowel disorders of unknown etiology. This study investigated phenotypic characteristics of the mucosal lymphocytes in CC and LC.

    Methods: Lamina propria and intraepithelial lymphocytes (LPLs, IELs) isolated from mucosal biopsies from CC (n = 7), LC (n = 6), as well as LC or CC patients in histopathological remission, (LC-HR) (n = 6) and CC-HR (n = 4) and non-inflamed controls (n = 10) were phenotypically characterized by four-color flow cytometry.

    Results: The proportions of CD8+ IELs were increased in CC and LC (p < 0.01) compared to controls. Increased proportions of CD45RO+CD8+ IELs and LPLs were observed in LC and even more in CC patients (p < 0.01). Both CC (p < 0.05) and LC patients had elevated proportions of CD4+8+ IELs and LPLs compared to controls. The proportions of CD45RO+ cells were increased in CD4+8+ IELs and LPLs (p < 0.05) in CC and LC patients compared to controls. Both CC (p < 0.05) and LC patients had higher proportions of Ki67+CD8+ IELs and LPLs compared to controls.

    In contrast, decreased proportions of CD4+ LPLs were observed in CC and LC as well as CD4+ IELs in LC compared to controls. Increased proportions of Ki67+CD4+ IELs and LPLs (p < 0.05) were observed in CC and LC patients. CC-HR but not LC-HR patients demonstrated normalized proportions of both IELs and LPLs compared to CC and LC patients respectively.

    Conclusion: LC and CC patients have differences in mucosal lymphocyte subsets, with increased proportions of Ki67+ and CD45RO+ CD8+ and CD4+8+ mucosal T cells.

  • 215.
    Kumawat, Ashok Kumar
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bohr, Johan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren, Olof
    Örebro universitet, Institutionen för läkarutbildning.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    An in vitro model for analysis of the impact of the colonic milieu in collagenous colitis patients on peripheral T lymphocyte activation and differentiationManuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Background: Soluble factors released by intestinal mucosal cells contribute to immune homeostasis in the gut. This is the first study to investigate the role of soluble factors from the intestinal mucosa of collagenous colitis (CC) patients in the regulation of effector T cells using a novel system that mimics the in vivo exposure of newly recruited peripheral blood T cells to soluble factors derived from the colonic milieu of normal individuals and inflamed CC patient mucosa.

    Methods: Denuded biopsies (DNB) and isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients and non-inflamed controls were cultured to collect conditioned medium (CM). Enriched peripheral blood CD4+ T cells from healthy donors were polyclonally activated in the absence or presence of CM from CC patients and controls. Proliferation, as well as secretion of IL-1β IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α was analysed the latter with Luminex® analysis.

    Results: Peripheral CD4+ T cells exposed to CM from the colonic mucosa demonstrated reduced proliferation. This inhibition was less pronounced with DNB-CM derived from CC patients compared to non-inflamed control mucosa. In contrast, LPMC-CM from non-inflamed controls inhibited T-cell proliferation less than LPMC-CM from CC patients. Both DNB-CM and LPMC-CM from CC patients induced more or less increased production of the proinflammatory cytokines IFN-γ, IL-17A, IL-6 and TNF-α as well as the anti-inflammatory cytokines IL-4 and IL-10 from peripheral CD4+ T cells compared to non-inflamed controls. In contrast, IL-1β production by peripheral T cells showed mixed results – it was either increased or reduced in the presence of both DNB and LPMC-CM from CC patients compared to noninflamed controls with different blood donors and different concentrations.

    Conclusion: Our preliminary data indicates reduced inhibition of proliferation of peripheral CD4+ T cells in the presence of mucosa-derived soluble factors from CC patients compared to controls. In addition, increased production of both inflammatory and anti-inflammatory cytokines by peripheral CD4+ T cells was recorded in the presence of soluble factors from the colonic mucosa of CC patients compared to controls. This model can be valuable in evaluating the effect(s) of existing and new drugs on T cell differentiation in the intestinal mucosa.

  • 216.
    Kumawat, Ashok
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro University Hospital, Örebro, Sweden.
    Bohr, Johan
    Örebro University Hospital, Örebro, Sweden.
    Hultgren, Olof
    Örebro University Hospital, Örebro, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    An in vitro model for analysis of the impact of the colonic milieu in collagenous colitis patients on peripheral T lymphocyte activation and differentiation2013Ingår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 140, s. 168-168Artikel i tidskrift (Övrigt vetenskapligt)
  • 217.
    Kämpe, Mary
    et al.
    Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, Uppsala, Sweden.
    Lisspers, Karin
    Department of Public Health and Caring Science, Family Medicine and Preventive Medicine, Uppsala University, Uppsala, Sweden.
    Ställberg, Björn
    Department of Public Health and Caring Science, Family Medicine and Preventive Medicine, Uppsala University, Uppsala, Sweden.
    Sundh, Josefin
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Deparment of Respiratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Montgomery, Scott
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Clinical Epidemiology Unit, Department of Medicine, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden; Department of Epidemiology and Public Health, University College London, London, UK.
    Jansson, Christer
    Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, Uppsala, Sweden.
    Determinants of uncontrolled asthma in a Swedish asthma population: cross-sectional observational study2014Ingår i: European Clinical Respiratory Journal, ISSN 2001-8525, Vol. 1, artikel-id 24109Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Asthma control is achieved in a low proportion of patients. The primary aim was to evaluate risk factors for uncontrolled asthma. The secondary aim was to assess quality of life associated with asthma control.

    Methods: In a cross-sectional study, asthma patients aged 18–75 were randomly selected from primary and secondary health care centers. Postal questionnaires were sent to 1,675 patients and the response rate was 71%. A total of 846 patients from primary and 341 patients from secondary care were evaluated. Data were collected using a questionnaire and review of medical records. The questionnaire included questions about asthma control and a quality-of-life questionnaire, the mini-AQLQ, with four domains (symptoms, activity limitation, emotional function, and environmental stimuli). The mean score for each domain and the overall score were calculated. Asthma control was divided into three levels according to the GINA guidelines and partly and uncontrolled asthma were combined into one group – poorly controlled asthma.

    Results: Asthma control was achieved in 36% of the sample: 38% in primary and 29% in secondary care. In primary and secondary care, 35 and 45% had uncontrolled asthma, respectively. Risk factors for poorly controlled asthma were female sex [OR 1.31 (1.003–1.70)], older age [OR 2.18 (1.28–3.73)], lower educational level [OR 1.63 (1.14–2.33)], and current smoking [OR 1.68 (1.16–2.43)]. Older age and lower educational level remained statistically significantly associated with poorly controlled asthma when the analyses were limited to never-smokers. Depression was an independent risk factor for poorly controlled asthma in men [OR 3.44 (1.12–10.54)]. The mini-AQLQ scores and the mean overall score were significantly lower in uncontrolled asthma.

    Conclusion: Risk factors for poorly controlled asthma were female sex, older age, low educational level, and smoking. Uncontrolled asthma was significantly associated with lower quality of life.

  • 218.
    König, Julia
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Brummer, Robert-Jan
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Alteration of the intestinal microbiota as a cause of and a potential therapeutic option in irritable bowel syndrome2014Ingår i: Beneficial Microbes, ISSN 1876-2883, E-ISSN 1876-2891, Vol. 5, nr 3, s. 247-261Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    The intestinal microbiota forms a complex ecosystem that is in close contact with its host and has an important impact on health. An increasing number of disorders are associated with disturbances in this ecosystem. Also patients suffering from irritable bowel syndrome (IBS) show an altered composition of their gut microbiota. IBS is a multifactorial chronic disorder characterised by various abdominal complaints and a worldwide prevalence of 10-20%. Even though its aetiology and pathophysiology are complex and not well understood, it is widely accepted that aberrations along the microbe-gut-brain axis are involved. In this review, it will be discussed how exogenous factors, e.g. antibiotics, can cause disbalance in the intestinal microbiota and thereby contribute to the development of IBS. In addition, several new IBS treatment options that aim at re-establishing a healthy, beneficial ecosystem will be described. These include antibiotics, probiotics, prebiotics and faecal transplantation.

  • 219.
    König, Julia
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Brummer, Robert-Jan
    Örebro universitet, Institutionen för läkarutbildning.
    Modulation of the gut ecosystem in irritable bowel syndrome2014Ingår i: Pharma-Nutrition: an overview / [ed] Gert Folkerts, Johan Garssen, Springer, 2014, s. 55-73Kapitel i bok, del av antologi (Refereegranskat)
  • 220.
    König, Julia
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Rangel, Ignacio
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Brummer, Robert Jan
    Örebro universitet, Institutionen för läkarutbildning.
    The Role of Lactic Acid Bacteria in the Pathophysiology and Treatment of Irritable Bowel Syndrome (IBS)2013Ingår i: Food and Nutrition Sciences, ISSN 2157-944X, E-ISSN 2157-9458, Vol. 4, nr 11, s. 27-39Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Irritable bowel syndrome (IBS) is a multifactorial chronic disorder characterized by various abdominal complaints and a worldwide prevalence of 10% - 20%. Although its etiology and pathophysiology are complex and still not completely understood, aberrations along the microbe-gut-brain axis are known to play a central role. IBS is characterized by inter-related alterations in intestinal barrier function, gut microbe composition, immune activation, afferent sensory signaling and brain activity. Pharmaceutical treatment is generally ineffective and, hence, most therapeutic strategies are based on non-drug approaches. A promising option is the administration of probiotics, in which lactic acid bacteria strains are considered specifically beneficial. This review aims to provide a concise, although comprehensive, overview of the role of lactic acid bacteria in the pathophysiology and treatment of IBS.

  • 221.
    Landström, Fredrik J
    et al.
    Department of Otolaryngology, Örebro University Hospital, Örebro, Sweden.
    Nilsson, Christer O. S.
    Department of Otolaryngology, Örebro University Hospital, Örebro, Sweden.
    Crafoord, Sven
    Örebro universitet, Institutionen för läkarutbildning. Department of Ophthalmology, Örebro University Hospital, Örebro, Sweden.
    Reizenstein, Johan A.
    Department of Oncology, Örebro University Hospital, Örebro, Sweden.
    Adamsson, Gun-Britt M.
    Department of Head and Neck Oncology Center, Örebro University Hospital, Örebro, Sweden.
    Löfgren, Lennart A.
    Department of Head and Neck Oncology Center, Örebro University Hospital, Örebro, Sweden.
    Electroporation therapy of skin cancer in the head and neck area.2010Ingår i: Dermatologic Surgery, ISSN 1076-0512, E-ISSN 1524-4725, Vol. 36, nr 8, s. 1245-50Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Electroporation therapy is a new cancer treatment modality in which a locally applied electrical field enhances cell membrane permeability, allowing greater intracellular accumulation of a chemotherapeutic agent.

    OBJECTIVE: To evaluate the efficacy of electroporation therapy in treating basal cell and squamous cell carcinomas of the skin.

    MATERIALS AND METHODS: Six patients with skin cancer of the head and neck were treated using electroporation therapy with intratumorally injected bleomycin. Orbital growth, facial nerve proximity, or proximity to cartilage of the external meatus complicated four of these tumors. The intention was curative. The follow-up period was 24 months and included biopsies after 8 weeks.

    RESULTS: In four of the six patients, one treatment was enough to eradicate the tumor. In one patient, the tumor persisted even after a second treatment with electroporation therapy. A septal cartilage perforation was the only major complication. The cosmetic results were very satisfactory. One additional recurrence was recorded 6 months after the follow-up period

    CONCLUSION: Electroporation therapy is a promising new cancer treatment that should be further evaluated as an alternative to surgery, especially in complicated skin cancer.

  • 222.
    Larsson, Matilda
    Örebro universitet, Institutionen för läkarutbildning.
    Antibiotic susceptibility patterns of ESBL-producing E. coli isolates from water environments and urine samples from patients2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 223.
    Lassen, K.
    et al.
    University Hospital Northern Norway, Tromsø, Norway; Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Dejong, C. H. C.
    Maastricht University Hospital , Maastricht, The Netherlands; School for Nutritition, Toxicology and Metabolism (NUTRIM ), Maastricht University Medical Centre+, Maastricht, The Netherlands.
    Demartines, N.
    University Hospital of Lausanne (CHUV), Lausanne, Switzerland.
    Parks, R W
    Royal Infirmary of Edinburgh, University of Edinburgh, Edinburgh, United Kingdom.
    Lobo, D. N.
    Nottingham University Hospitals, Queen's Medical Centre, Nottingham, United Kingdom.
    Coolsen, M. M. E.
    Maastricht University Hospital , Maastricht, The Netherlands; School for Nutritition, Toxicology and Metabolism (NUTRIM ), Maastricht University Medical Centre+, Maastricht, The Netherlands.
    Fearon, K. C. H.
    Royal Infirmary of Edinburgh, University of Edinburgh, Edinburgh, United Kingdom.
    Pancreaticoduodenectomy: ERAS recommendations.2013Ingår i: Clinical Nutrition, ISSN 0261-5614, E-ISSN 1532-1983, Vol. 32, nr 5, s. 870-871Artikel i tidskrift (Refereegranskat)
  • 224. Lassen, Kristoffer
    et al.
    Coolsen, Marielle M. E.
    Slim, Karem
    Carli, Francesco
    de Aguilar-Nascimento, José E.
    Schäfer, Markus
    Parks, Rowan W.
    Fearon, Kenneth C. H.
    Lobo, Dileep N.
    Demartines, Nicolas
    Braga, Marco
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Dejong, Cornelis H. C.
    Guidelines for perioperative care for pancreaticoduodenectomy: enhanced recovery after surgery (ERAS®) society recommendations2013Ingår i: World Journal of Surgery, ISSN 0364-2313, E-ISSN 1432-2323, Vol. 37, nr 2, s. 240-258Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Protocols for enhanced recovery provide comprehensive and evidence-based guidelines for best perioperative care. Protocol implementation may reduce complication rates and enhance functional recovery and, as a result of this, also reduce length-of-stay in hospital. There is no comprehensive framework available for pancreaticoduodenectomy.

    METHODS: An international working group constructed within the Enhanced Recovery After Surgery (ERAS(®)) Society constructed a comprehensive and evidence-based framework for best perioperative care for pancreaticoduodenectomy patients. Data were retrieved from standard databases and personal archives. Evidence and recommendations were classified according to the GRADE system and reached through consensus in the group. The quality of evidence was rated "high", "moderate", "low" or "very low". Recommendations were graded as "strong" or "weak".

    RESULTS: Comprehensive guidelines are presented. Available evidence is summarised and recommendations given for 27 care items. The quality of evidence varies substantially and further research is needed for many issues to improve the strength of evidence and grade of recommendations.

    CONCLUSIONS: The present evidence-based guidelines provide the necessary platform upon which to base a unified protocol for perioperative care for pancreaticoduodenectomy. A unified protocol allows for comparison between centres and across national borders. It facilitates multi-institutional prospective cohort registries and adequately powered randomised trials.

  • 225.
    Lassen, Kristoffer
    et al.
    Department of Gastrointestinal and Hepatopancreatobiliary Surgery, University Hospital Northern Norway, Tromsø, Norway; Institute of Clinical Medicine, Arctic University of Norway, Tromsø, Norway.
    Dejong, Cornelis H.
    Department of Surgery, Maastricht University Medical Centre, Maastricht, The Netherlands; NUTRIM School for Nutrition Toxicology and Metabolism, Maastrich University, Maastricht, The Netherlands.
    Revhaug, Arthur
    Department of Gastrointestinal and HepatoPancreatoBiliary Surgery, University Hospital Northern Norway, Tromsø, Norway; Institute of Clinical Medicine, Arctic University of Norway, Tromsø, Norway.
    Fearon, Ken
    Department of Clinical and Surgical Sciences, University of Edinburgh, Edinburgh, United Kingdom.
    Lobo, Dileep N.
    Gastrointestinal Surgery, Nottingham Digestive Diseases Centre, Queen’s Medical Centre Nottingham, Nottingham University Hospitals, Nottingham, United Kingdom.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning.
    Food at will after pancreaticoduodenectomies. Re. "Perioperative nutritional support of patients undergoing pancreatic surgery in the age of ERAS"2015Ingår i: Nutrition (Burbank, Los Angeles County, Calif.), ISSN 0899-9007, E-ISSN 1873-1244, Vol. 31, nr 7-8, s. 1057-1058Artikel i tidskrift (Refereegranskat)
  • 226. Lehmann, S
    et al.
    Bykov, VJ
    Ali, D
    Andrén, Ove
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Cherif, H
    Tidefelt, Ulf
    Örebro universitet, Institutionen för läkarutbildning.
    Uggla, Bertil
    Örebro universitet, Institutionen för läkarutbildning.
    Yachnin, J
    Juliusson, G
    Moshfegh, A
    Paul, C
    Wiman, KG
    Andersson, PO
    Targeting p53 in vivo: a first-in-man study with the p53-targeting compound APR-246 in refractory hematologic malignancies and prostate cancer2012Ingår i: Journal of Clinical Oncology, ISSN 0732-183X, E-ISSN 1527-7755, Vol. 30, nr 29, s. 3633-3639Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE: APR-246 (PRIMA-1MET) is a novel drug that restores transcriptional activity of unfolded wild-type or mutant p53. The main aims of this first-in-human trial were to determine maximum-tolerated dose (MTD), safety, dose-limiting toxicities (DLTs), and pharmacokinetics (PK) of APR-246.

    PATIENTS AND METHODS: APR-246 was administered as a 2-hour intravenous infusion once per day for 4 consecutive days in 22 patients with hematologic malignancies and prostate cancer. Acute myeloid leukemia (AML; n = 7) and prostate cancer (n = 7) were the most frequent diagnoses. Starting dose was 2 mg/kg with dose escalations up to 90 mg/kg.

    RESULTS: MTD was defined as 60 mg/kg. The drug was well tolerated, and the most common adverse effects were fatigue, dizziness, headache, and confusion. DLTs were increased ALT/AST (n = 1), dizziness, confusion, and sensory disturbances (n = 2). PK showed little interindividual variation and were neither dose nor time dependent; terminal half-life was 4 to 5 hours. Tumor cells showed cell cycle arrest, increased apoptosis, and upregulation of p53 target genes in several patients. Global gene expression analysis revealed changes in genes regulating proliferation and cell death. One patient with AML who had a p53 core domain mutation showed a reduction of blast percentage from 46% to 26% in the bone marrow, and one patient with non-Hodgkin's lymphoma with a p53 splice site mutation showed a minor response.

    CONCLUSION: We conclude that APR-246 is safe at predicted therapeutic plasma levels, shows a favorable pharmacokinetic profile, and can induce p53-dependent biologic effects in tumor cells in vivo.

  • 227.
    Lennholm, Sophie
    Örebro universitet, Institutionen för läkarutbildning.
    Use of antibiotics and development of asthma and atopic disease2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 228.
    Lidén, Mats
    et al.
    Örebro universitet, Hälsoakademin.
    Andersson, Torbjörn
    Örebro universitet, Institutionen för läkarutbildning.
    Geijer, Håkan
    Örebro universitet, Hälsoakademin.
    Making renal stones change size: impact of CT image post processing and reader variability2011Ingår i: European Radiology, ISSN 0938-7994, E-ISSN 1432-1084, Vol. 21, nr 10, s. 2218-2225Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objectives The objectives of this study were to quantify the impact of image post-processing parameters on the apparent renal stone size, and to quantify the intra- and inter-reader variability in renal stone size estimation. Methods Fifty CT datasets including a renal or ureteral stone were included retrospectively during a prospective inclusion period. Each of the CT datasets was post-processed in different ways regarding slice thickness, slice increment and window setting. In the first part of the study a single reader repeated size estimations for the renal stones using different post-processing parameters. In the intra-reader variability experiment one reader reported size estimations for the same images with a one-week interval. The inter-reader variability data were obtained from 11 readers reporting size estimations for the same renal stones. Results The apparent stone size differed according to image post-processing parameters with the largest mean differences seen with regard to the window settings experiment (1.5 mm, p < 0.001) and slice thickness (0.8 mm, p < 0.001). Changes in parameters introduced a bias and a pseudo-random variability. The inter-reader variability was considerably larger than the intra-reader variability. Conclusion Our results indicate a need for the standardisation of making measurements on CT images.

  • 229.
    Lidén, Mats
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Andersson, Torbjörn
    Örebro universitet, Institutionen för läkarutbildning.
    Geijer, Håkan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Urinary stone size estimation: Can we reduce the reader variations?Manuskript (preprint) (Övrigt vetenskapligt)
  • 230.
    Lillsunde-Larsson, Gabriella
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Carlsson, Jessica
    Karlsson, Mats G.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital.
    HPV genotyping assays for archival clinical samples: an evaluation studyManuskript (preprint) (Övrigt vetenskapligt)
  • 231.
    Lillsunde-Larsson, Gabriella
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital, Örebro, Sweden.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Örebro, Sweden.
    Andersson, Sören
    Örebro University Hospital, Örebro, Sweden.
    Elgh, Fredrik
    Umeå University Hospital, Umeå, Sweden.
    Sorbe, Bengt
    Örebro universitet, Hälsoakademin. Örebro University Hospital, Örebro, Sweden.
    Karlsson, Mats G.
    Örebro University Hospital, Örebro, Sweden.
    Human Papillomavirus (HPV) and HPV 16-Variant Distribution in Vulvar Squamous Cell Carcinoma in Sweden2012Ingår i: International Journal of Gynecological Cancer, ISSN 1048-891X, E-ISSN 1525-1438, Vol. 22, nr 8, s. 1413-1419Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective: To investigate the human papillomavirus (HPV) and HPV type 16-variant distribution in a series of vulvar squamous cell carcinomas (VSCC) and to evaluate the impact of HPV and HPV 16-variant on prognosis.

    Methods: A series of 133 patients who had a diagnosis of VSCC (1983-2008) was selected for the study. Detection of 11 high-risk HPV types (16, 18, 31, 33, 39, 45, 51, 52, 56, 58, and 59) and 2 low-risk HPV types (6 and 11) was performed with real-time polymerase chain reaction. Samples positive for HPV 16 were further analyzed for variant determination of 7 positions in the E6 gene with polymerase chain reaction and pyrosequencing.

    Results: Forty (30.8%) of 130 tumors were found to be HPV positive. Human papillomavirus type 16 was found in 31 cases, HPV 18 was found in 2 cases, HPV 33 was found in 5 cases, and HPV 56 and HPV 59 were found in one case each. All but one tumor harboring HPV 16 were of European linage, and the 3 most common variants were E-p (n = 13), E-G350 (n = 7), and E-G131 (n = 5). HPV positivity was associated with the basaloid tumor type and occurred in significantly younger patients. Overall and recurrence-free survival rates were better in HPV-positive cases, but after correction for age and tumor size, HPV status was no longer an independent and significant prognostic factor. The survival rates of the various HPV 16 variants were not significantly different, but there was a trend of worse outcome for the E-G131-variant group.

    Conclusions: Human papillomavirus positivity of 30.8% is similar to other reports on VSCC. To our knowledge, this first variant determination of HPV 16 in vulvar carcinoma in a Swedish cohort indicated that the variant E-G131 may have an increased oncogenic potential in patients with VSCC.

  • 232.
    Lillsunde-Larsson, Gabriella
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Andersson, Sören
    Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Sorbe, Bengt
    Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Karlsson, Mats G.
    Region Örebro län. Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Prognostic impact of human papilloma virus (HPV) genotyping and HPV-16 subtyping in vaginal carcinoma2013Ingår i: Gynecologic Oncology, ISSN 0090-8258, E-ISSN 1095-6859, Vol. 129, nr 2, s. 406-411Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective

    The objectives of this study are to investigate the human papilloma virus (HPV) distribution in vaginal cancer and to evaluate HPV-genotype as well as HPV16-variant impact on prognosis.

    Methods

    Sixty-nine patients diagnosed with primary vaginal carcinoma (1975-2002) were included in the study. Detection of twelve high-risk HPV (hr HPV) and two low-risk HPV (lr HPV) was performed with realtime-PCR. Samples positive for HPV-16 were analyzed for variants in the E6-gene with PCR and pyrosequencing.

    Results

    53.6% (37/69) of the tumors were found to be HPV-positive, mostly for HPV-16 (N=26). Other HPV-types were HPV-18 (N=2), HPV-31 (N=2), HPV-33 (N=2), HPV-45 (N=1), HPV-52 (N=2), HPV-56 (N=1) and HPV-58 (N=1). Only European subtypes of HPV-16 were represented and the two most common HPV-16-variants were E-p (N=13) and E-G350 (N=11). Patients with HPV-positive tumors (N=37) had a significantly (log-rank test=3341; p = 0.0008) superior 5-year overall survival rate as well as cancer-specific survival rate and progression-free survival rate (p = 0.0002; p = 0.0004), compared with patients with HPV-negative tumors (N=32). Interestingly, patients with HPV-16-positive tumors had a superior overall survival compared with patients with tumors containing other HPV-genotypes. In a Cox proportional multivariate analysis age, tumor size, and HPV-status were independent and significant prognostic factors with regard to overall survival rate.

    Conclusions

    HPV-status is of prognostic importance in vaginal carcinoma and varies with viral genotype. In this era of HPV-vaccination, genotypes other than those included in the vaccination program could still lead to vaginal carcinoma with unfavorable prognosis.

  • 233.
    Lillsunde-Larsson, Gabriella
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Sorbe, Bengt
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Oncology, Örebro University Hospital, Örebro, Sweden.
    Karlsson, Mats G.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Viral Load, Integration and Methylation of E2BS3 and 4 in Human Papilloma Virus (HPV) 16-Positive Vaginal and Vulvar Carcinomas2014Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, nr 11, artikel-id e112839Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective: To investigate if viral load, integration and methylation of E2BS3 and 4 represent different ways of tumor transformation in vaginal and vulvar carcinoma and to elucidate its clinical impact.

    Methods: Fifty-seven samples, positive for HPV16, were selected for the study. Detection of viral load was made with realtime-PCR using copy numbers of E6 and integration was calculated from comparing E2 to E6-copies. Methylation of E2BS3 and 4 was analysed using bisulphite treatment of tumor DNA, followed by PCR and pyrosequencing.

    Results: Vaginal tumors were found to have a higher viral load (p=0.024) compared to vulvar tumors but a high copy number (> median value, 15 000) as well as high methylation (> 50%) was significantly (p=0.010 and p=0.045) associated with a worse cancer-specific survival rate in vulvar carcinoma, but not in vaginal carcinoma. Four groups could be defined for the complete series using a Cluster Two step analysis; (1) tumors holding episomal viral DNA, viral load below 150 000 copies not highly methylated (n=25, 46.3%); (2) tumors harboring episomal viral DNA and being highly methylated (>50%; n=6, 11.1%); (3) tumors with viral DNA fully integrated (n=11, 20.4%), and (4) tumors harboring episomal viral DNA and being medium-or unmethylated (< 50%) and having a high viral load (> total mean value 150 000; n=12, 22.2%). The completely integrated tumors were found to be distinct group, whilst some overlap between the groups with high methylation and high viral load was observed.

    Conclusion: HPV16-related integration, methylation in E2BS3 and 4 and viral load may represent different viral characteristics driving vaginal and vulvar carcinogenesis. HPV16-related parameters were found to be of clinical importance in the vulvar series only.

  • 234.
    Lind, Henrietta
    Örebro universitet, Institutionen för läkarutbildning.
    A retrospective journal-based study of patients diagnosed with secondary hemophagocytic lymphohistiocytosis at USÖ during 2000-20142015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 235.
    Lind, Patrik
    Örebro universitet, Institutionen för läkarutbildning.
    Prophylactic Antibiotic and Antitrombotic treatment in patients undergoing Parathyroid and Thyroid surgery at the Department of Surgery Örebro University Hospital 2009-2012.: Registration of bleeding, infectious and trombotic complications.2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 236.
    Lindberg, Magnus
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Department of Dermatology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Bingefors, Kerstin
    Institute of Environmental Medicine (IMM), Karolinska Institutet, Stockholm, Sweden.
    Meding, Birgitta
    Department of Pharmacy, Uppsala University, Uppsala, Sweden.
    Berg, Mats
    Centre for Clinical Research Sörmland, Eskilstuna, Sweden; Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
    Hand eczema and health-related quality of life: a comparison of EQ-5D and the Dermatology Life Quality Index (DLQI) in relation to the hand eczema extent score (HEES)2013Ingår i: Contact Dermatitis, ISSN 0105-1873, E-ISSN 1600-0536, Vol. 69, nr 3, s. 138-143Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Health-related quality of life (HRQoL) is associated with the extent and severity of hand eczema. We still lack a consensus about which HRQoL instrument to use as the standard, and how to measure the extent and severity of hand eczema.

    Objectives: To compare the Dermatology Life Quality Index (DLQI) with EQ-5D (a standardized instrument for use as a measure of health outcome), and to evaluate how the Hand Eczema Extent Score (HEES) relates to these instruments.

    Methods: Ninety-three patients (61 females) were included. The HEES was recorded by a dermatologist, and the DLQI and EQ-5D by the patients. The results were analysed with factor analysis and non-parametric statistics.

    Results: The DLQI and EQ-5D showed decreased HRQoL. Using factor analysis, we could not establish an association between the DLQI and EQ-5D. There were, however, correlations between the DLQI and the HEES (0.31), the EQ(index) and the HEES (-0.32), the DLQI and the EQ(VAS) (-0.62), and the DLQI and the EQ(index) (-0.67) (the EQ(VAS) and the EQ(index) are calculated from EQ-5D).

    Conclusions: We could not link factors in the DLQI to EQ-5D, which has previously been done for SF-36 (Short Form 36). On the basis of this, we cannot recommend EQ-5D over SF-36 for hand eczema studies. The DLQI correlates with the EQ(VAS) and the EQ(index), and can probably be used as an approximation for EQ-5D. Our findings with the HEES are interesting, as it can be used by patients.

  • 237.
    Lindh, Oskar
    Örebro universitet, Institutionen för läkarutbildning.
    Hur går det för patienter som är mögelsensibiliserade?2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 238.
    Lindhe, Oskar
    Örebro universitet, Institutionen för läkarutbildning.
    Resistance exercise could be a better option, for type 1 diabetic youths, than aerobic exercise to prevent hypoglycaemia during workout2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 239.
    Lindmark, Gustav
    Örebro universitet, Institutionen för läkarutbildning.
    Serious complications with ECT: A prospective study of Swedish clinical practice.2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 240.
    Lindqvist, Breezy Malakkaran
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Farkas, Sanja A.
    Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    Nilsson, Torbjörn K.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    DNA methylation pattern of the SLC25A43 gene in breast cancer2012Ingår i: Epigenetics, ISSN 1559-2294, E-ISSN 1559-2308, Vol. 7, nr 3, s. 300-306Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Solute carrier family 25A member 43 (SLC25A43) gene is a putative tumor suppressor gene that undergoes loss of heterozygosity (LOH) in human epidermal growth factor receptor 2 (HER2) positive breast cancer. Also, knockdown of SLC25A43 in cell lines influences cell turnover and metabolism. Absence of mutations in this gene in breast cancers prompted us to study methylation as an alternate mechanism for gene inactivation of this X encoded gene. Quantification of CpG site methylation using pyrosequencing was performed upstream of the SLC25A43 gene and at its 5' end in a cohort of breast tumor tissues (n = 80, HER2 positive or negative) with different SLC25A43 gene deletion status. Compared with control tissue, cancer tissues had lower levels of methylation at the 5' and 3' shores of the gene. Cancer tissues with no deletion in the SLC25A43 gene (Del(-)) had higher methylation in the CpG island (CGI) of the gene than cancers carrying the deletion (Del(+)). Methylation in the CGI of the SLC25A43 gene was negatively correlated with age at diagnosis. In HER2 positive breast cancer, ER negativity and lymph node positivity was associated with higher methylation in the CGI and in the adjacent shores of this gene. Our results suggest that methylation in the CGI of the SLC25A43 gene could be an alternate mechanism of gene silencing in the absence of LOH. Also, associations between site-specific methylation and clinicopathological parameters suggest that epigenetic changes in SLC25A43 gene could be of importance in breast carcinogenesis.

  • 241.
    Lindqvist, Breezy Malakkaran
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Gyamfi, Jones
    Jakobsson, Frida
    Department of Oncology, Örebro University Hospital, SE-70185 Örebro, Sweden.
    Tina, Elisabet
    Clinical Research Centre, Örebro University Hospital, SE-70185 Örebro, Sweden.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    Cytoplasmic pFOXO3a expression is associated with sentinel node metastasis in HER2-positive breast cancerManuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Introduction: Breast cancers with human epidermal growth factor receptor (HER) 2 gene amplification or protein overexpression (HER2+ breast cancer) is associated with poor prognosis. Activated HER2 receptors dimerise and activate the oncokinase Akt; which phosphorylate the tumour suppressor protein Forkhead box O3a (FOXO3a), repressing its transcriptional activity. Oncogenic FOXG1 can act as a transcriptional repressor for genes which are transcriptionally activated by FOXOs and phosphorylation of FOXG1 via Akt causes its nuclear export in differentiated cells. To better understand the AKT/FOXO3a/FOXG1 connection and their clinical relevance, we investigated the expression and localisation of pAkt, pFOXO3a and FOXG1 in HER2+ breast cancer.

    Methods: Immunohistochemical analysis was performed to determine the expression and localisation of pAkt, pFOXO3a and FOXG1 on tissue microarray constructs from HER2+ primary breast tumours (n=91) and their relation to clinic pathological parameters were analysed.

    Results: Nuclear expression of pAkt was found to be correlated to nuclear (p<0.001) as well as cytoplasmic expression of pFOXO3a (p = 0.006), while cytoplasmic expression of pAkt was found to be correlated to cytoplasmic expression of pFOXO3a (p<0.001). Nuclear expression of pFOXO3a was inversely correlated to cytoplasmic expression of FOXG1 (p=0.003). Cytoplasmic expression of pFOXO3a was found to be associated with sentinel node metastasis (p=0.011), while cytoplasmic FOXG1 expression was correlated to negative progesterone receptor status (p=0.008).

    Conclusion: Our results suggest that the expression and localisation of pAkt and pFOXO3a is interconnected, while the expression of FOXG1 is connected to pFOXO3a. Our findings indicate the biological value of expression as well as localisation of these proteins in HER2+ breast cancer.

  • 242.
    Lindqvist, Breezy Malakkaran
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    Motlagh, Parviz B.
    Department of Medical Biosciences/Clinical Chemistry, Umeå University, Umeå, Sweden.
    Nilsson, Torbjorn K.
    Department of Medical Biosciences/Clinical Chemistry, Umeå University, Umeå, Sweden.
    Whole genome DNA methylation signature of HER2-positive breast cancer2014Ingår i: Epigenetics, ISSN 1559-2294, E-ISSN 1559-2308, Vol. 9, nr 8, s. 1149-1162Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In order to obtain a comprehensive DNA methylation signature of HER2-positive breast cancer (HER2+ breast cancer), we performed a genome-wide methylation analysis on 17 HER2+ breast cancer and compared with ten normal breast tissue samples using the Illumina Infinium HumanMethylation450 BeadChip (450K). In HER2+ breast cancer, we found altered DNA methylation in genes involved in multicellular development, differentiation and transcription. Within these genes, we observed an overrepresentation of homeobox family genes, including several genes that have not been previously reported in relation to cancer (DBX1, NKX2-6, SIX6). Other affected genes included several belonging to the PI3K and Wnt signaling pathways. Notably, HER2, AKT3, HK1, and PFKP, genes for which altered methylation has not been previously reported, were also identified in this analysis. In total, we report 69 candidate biomarker genes with maximum differential methylation in HER2+ breast cancer. External validation of gene expression in a selected group of these genes (n = 13) revealed lowered mean gene expression in HER2+ breast cancer. We analyzed DNA methylation in six top candidate genes (AKR1B1, INA, FOXC2, NEUROD1, CDKL2, IRF4) using EpiTect Methyl II Custom PCR Array and confirmed the 450K array findings. Future clinical studies focusing on these genes, as well as on homeobox-containing genes and HER2, AKT3, HK1, and PFKP, are warranted which could provide further insights into the biology of HER2+ breast cancer.

  • 243.
    Lindqvist, Breezy Malakkaran
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Wingren, Sten
    Örebro universitet, Institutionen för läkarutbildning.
    Nilsson, Torbjörn
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Whole genome DNA methylation signature of HER2-positive breast cancerManuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    With the aim of obtaining a more comprehensive epigenetic signature in HER2-positive breast cancer (HER2+ breast cancer), we performed a genome-wide methylation analysis on 17 HER2+ breast cancer and compared to 10 normal breast tissue samples using the Illumina Infinium HumanMethylation450 BeadChip that interrogates >485,000 CpG loci per sample. Our findings show that altered DNA methylation, more specifically hypermethylation occur at CpG islands in HER2+ breast cancer affecting genes involved in multicellular development, differentiation and transcription, and was overrepresented by the homeobox family of genes, including those which have not been previously reported in relation to cancer (DBX1, NKX2- 6, SIX6). Alteration in methylation was also found to affect genes of the PI3K and the Wnt signalling pathways in HER2+ breast cancer. Notably among them are HER2, AKT3, HK1 and PFKP, in which altered methylation has not been previously reported. We have identified 73 candidate biomarker genes in HER2+ breast cancer and external validation of gene expression in a selected group of these genes (n=13) revealed lowered mean gene expression in HER2+ breast cancer. Future clinical studies focusing on these candidate biomarker genes as well as homeobox-containing genes and HER2, AKT3, HK1 and PFKP are warranted which could provide further insights to the biology of HER2+ breast cancer.

  • 244.
    Lindén Wikblom, Anna
    Örebro universitet, Institutionen för läkarutbildning.
    Antikoagulation och förmaksflimmer En utvärdering av följsamhet till nationella riktlinjer i samband med elkonvertering, Kardiologiska kliniken, USÖ, 20122015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 245.
    Lingroth Karlsson, Annika
    Örebro universitet, Institutionen för läkarutbildning.
    Kapillär återfyllnad som undersökningmetod -en pilotstudie2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 246.
    Lisspers, Karin
    et al.
    Family Medicine & Preventive Medicine, Uppsala University, Uppsala, Sweden; Department of Public Health and Caring Sciences, Uppsala University, Uppsala, Sweden.
    Janson, Christer
    Resp Med & Allergol, Med Sci, Uppsala University, Uppsala, Sweden.
    Sundh, Josefin
    Örebro universitet, Institutionen för läkarutbildning. Department of Respiratory Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Montgomery, Scott
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kampe, Mary
    Resp Med & Allergol, Med Sci, Uppsala University, Uppsala, Sweden.
    Österlund, Eva
    Sch Hlth & Social Studies, Dalarna University, Falun, Sweden.
    Ericson, Anna
    Family Med & Prevent Med, Publ Hlth & Caring Sci, Uppsala Uiniversity, Uppsala, Sweden.
    Stallberg, Bjorn
    Family Med & Prevent Med, Publ Hlth & Caring Sci, Uppsala Uiniversity, Uppsala, Sweden.
    A follow-up of patients with a new diagnosis of asthma - characteristics, prognosis and risk factors2013Ingår i: European Respiratory Journal, ISSN 0903-1936, E-ISSN 1399-3003, Vol. 42, nr 57, artikel-id 3457Artikel i tidskrift (Övrigt vetenskapligt)
  • 247.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Surgery, Örebro University Hospital, Örebro, Sweden.
    ERAS-enhanced recovery after surgery: moving evidence-based perioperative care to practice2014Ingår i: JPEN - Journal of Parenteral and Enteral Nutrition, ISSN 0148-6071, E-ISSN 1941-2444, Vol. 38, nr 5, s. 559-566Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    ERAS is the acronym for enhanced recovery after surgery, a term often used to describe perioperative care programs that have been shown to improve outcomes after major surgery. This article gives a brief history of the development from fast-track surgery to ERAS. Today, the full meaning of ERAS goes beyond just a protocol for perioperative care with the initiation of a novel multiprofessional, multidisciplinary medical society: the Enhanced Recovery After Surgery Society for Perioperative Care (www. erassociety. org). The ERAS Society is involved in the development of evidence-based guidelines. These guidelines form the basis for an implementation program of the ERAS principles to practice. While ERAS was initially developed for colonic resections, these principles are being used in a range of operations, and there is also a continuous update of care protocols as the fields develop. A key mechanism behind the effectiveness of ERAS is the dampening of the stress responses to the surgical insult combined with the use of treatments that support return of functions that delay recovery in traditional care. The article also gives some insights to why the protocols work and reports the effects of ERAS protocols.

  • 248.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning.
    Jonathan E. Rhoads lecture 2011: insulin resistance and enhanced recovery after surgery2012Ingår i: JPEN - Journal of Parenteral and Enteral Nutrition, ISSN 0148-6071, E-ISSN 1941-2444, Vol. 36, nr 4, s. 389-398Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This lecture reviews the current understanding of how insulin resistance, as a marker of the metabolic stress, is involved in recovery after major surgery. Insulin resistance develops as a graded response related to the magnitude of the operation. It lasts for weeks after medium-size surgery and affects all parts of body metabolism. Although hyperglycemia develops, muscle and fat uptake is reduced and other non-insulin-sensitive cells have an increase in glucose uptake as a result of the elevated glucose levels. Reduced glucose uptake and storage in muscle along with loss of lean body mass help explain reduced muscle function that will impair mobilization. The increased uptake of glucose in non-insulin-sensitive cells is involved in the development of several of the most common postoperative complications, including infections and cardiovascular problems. Many of the perioperative treatments in use are outdated, and modern care involves a multimodal approach with several treatments, such as preoperative carbohydrate treatment instead of overnight fasting, continuous epidural anesthesia for postoperative pain care, early feeding, and mobilization, all of which affect insulin by reducing the stress and enhancing recovery. Most of the previous mandatory catabolic responses to surgery can be avoided, resulting in substantially faster recovery and fewer complications. Methods to implement these modern treatments have been developed and used in Europe, resulting in improved care and shorter length of stay.

  • 249.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning.
    Sustainability After Structured Implementation of ERAS Protocols2015Ingår i: World Journal of Surgery, ISSN 0364-2313, E-ISSN 1432-2323, Vol. 39, nr 2, s. 534-535Artikel i tidskrift (Refereegranskat)
  • 250.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning.
    The metabolic stress response and enhanced recovery2012Ingår i: Manual of fast track recovery for colorectal surgery / [ed] Nader Francis, Robin H. Kennedy, Olle Ljungqvist, Monty G. Mythen, London: Springer , 2012, s. 37-47Kapitel i bok, del av antologi (Övrigt vetenskapligt)
2345678 201 - 250 av 464
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf