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  • 51.
    Göthe, Johannes
    Örebro University, School of Health Sciences.
    Jämförelse och utvärdering av två kommersiella PCR kit för detektion av HLA-B*272017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 52.
    Hadi, Hadi
    Örebro University, School of Health Sciences.
    The correlation between consumption of benzodiazipines and fall-tendency in elderly people: A literature study2016Independent thesis Advanced level (degree of Master (One Year)), 30 credits / 45 HE creditsStudent thesis
  • 53.
    Haji Rashid, Shilan
    Örebro University, School of Health Sciences.
    Kvantifiering av humant papillomvirus typ 31 och 39 med droplet digital PCR.2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 54.
    Hallersjö, Alexander
    Örebro University, School of Health Sciences.
    Histokemisk/immunhistokemisk analys av odlingsmodell för broskreparation2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 55.
    Hallgren, Emil
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Effekter av stimulering med olika stammar av Phorphyromonas gingivalis på trombocyters aggregation med och utan hämning av PLSβ signalvägen2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 56.
    Halvarsson, Victoria
    Örebro University, School of Health Sciences.
    Effektivt avlägsnande av falskt positiva resultat för lupusantikoagulans i analysen dilute Russell’s viper venom time genom DOAC-StopTM för patienter som behandlas med direktverkande orala antikoagulantia2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 57.
    Hansen, Marit
    Örebro University, School of Health Sciences.
    Clinical and analytical evaluation of the APTIMA Mycoplasma genitalium assay on the Panther instrument (HOLOGIC)2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Objectives Mycoplasma genitalium (MG) is a frequent etiology of urethritis and cervicitis, which can result in severe reproductive complications. Resistance in MG to first-line (azithromycin) and second-line (moxifloxacin) treatment has rapidly increased. Our aims were to examine the sensitivity and specificity of the new CE/IVD APTIMA MG assay (Hologic) and a research-use-only (RUO) APTIMA MG assay; and the prevalence of MG, C. trachomatis and N. gonorrhoeae; in Denmark, Norway and Sweden in 2016.

    Methods From February 2016 to February 2017, urogenital specimens and extra-genital specimens (only in Denmark) from consecutive attendees at three STD clinics in Denmark, Norway and Sweden, were tested with the new CE/IVD APTIMA MG assay, RUO APTIMA MG assay, APTIMA CT/NG assay, and an in house TaqMan real-time mgpB qPCR. For analytical evaluation, genome-sequenced MG and other mycoplasma species in different concentration were tested.

    Results In total, 5269 evaluable patients were included. The rate of MG infected patients was 7.2% (4.9-9.8% in the different countries). The sensitivity of the CE/IVD APTIMA MG assay, RUO APTIMA MG assay and mgpB qPCR ranged between 99.1-100%, 99.1-100%, and 73.2-81.6%, respectively, in the countries. The specificity of the CE/IVD APTIMA MG assay, RUO APTIMA MG assay and mgpB qPCR ranged between 99.6-99.96%, 100%, and 99.7-100%, respectively.

    Conclusions Both the APTIMA MG assays had a superior sensitivity compared to the mgpB qPCR. The prevalence of MG was high and increased testing using validated and quality assured molecular tests for MG is crucial.

  • 58.
    Haque, Naba
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Visual Accommodation and its relation to pupil size, interpupillary distance and covergence in normal healthy individuals of age 20-35 years2012Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
  • 59.
    Hassan, Maryan
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Bestämning av okonjugerande metotoxikatekolaminer i urin: optimering av diagnostiken för feokromocytom2012Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 60.
    Hedberg, Sara Thulin
    et al.
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Törös, Bianca
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Fredlund, Hans
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Olcén, Per
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Mölling, Paula
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Genetic characterisation of the emerging invasive Neisseria meningitidis serogroup Y in Sweden, 2000 to 20102011In: Eurosurveillance, ISSN 1025-496X, E-ISSN 1560-7917, Vol. 16, no 23, article id 19885Article in journal (Refereed)
    Abstract [en]

    Neisseria meningitidis serogroups B and C have beenresponsible for the majority of invasive meningococcaldisease in Europe. Recently, an increase of N. meningitidisdisease due to serogroup Y has been notedin Sweden (in 2010, the proportion was 39%, with anincidence of 0.23 per 100,000 population), as well as inother northern European countries. We aimed to investigatethe clonal pattern of the emerging serogroup Yin Sweden during 2000 to 2010. The serogroup Y isolatesidentified during this time (n=85) were characterisedby multilocus sequence typing and sequencing ofthe fetA, fHbp, penA, porA and porB genes. The mostfrequent clone (comprising 28 isolates) with identicalallele combinations of the investigated genes, waspartly responsible for the observed increased numberof N. meningitidis serogroup Y isolates. It was sulfadiazineresistant, with genosubtype P1.5-2,10-1,36-2,sequence type 23, clonal complex 23, porB allele 3-36,fetA allele F4-1, fHbp allele 25 and penA allele 22. Thefirst case with disease due to this clone was identifiedin 2002: there was a further case in 2004, six during2006 to 2007, eight during 2008 to 2009, with a peakof 12 cases in 2010. An unusual increase of invasivedisease in young adults (aged 20–29 years) caused bythis clone was shown, but no increase in mortality ratewas observed.

  • 61.
    Hedlund, Hanna
    Örebro University, School of Health Sciences.
    Heat shock protein 70 as a marker for death by hypothermia2019Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Death due to hypothermia can be difficult to diagnose. Typically, the diagnosis is based on circumstances that suggest hypothermia in combination with certain findings such as stress ulcerations in the gastric mucosa, frost erythema and well preserved microscopic morphology of pancreas. Different stress factors, e.g. cold temperature, increases synthesis of a family of proteins called heat shock proteins (HSP). HSP70 expression in podocytes in the kidney of fatal hypothermia victims has previously been observed. In this study, the pattern of HSP70 in autopsy samples from several organs was studied in deaths due to hypothermia and in deaths due to other causes of death in a cold environment and in room temperature using HSP70 immunohistochemistry. An association was found between fatal hypothermia and HSP70 expression in the myocardium and the kidney. The pattern of specific podocyte nuclei positivity in fatal hypothermia cases previously reported was confirmed. Positivity in cardiomyocyte nuclei showed a less strong association with hypothermia. For all other organs, certain types of cells were positive regardless of cold exposure or not. The same was true for the positivity in renal tubule epithelium. Cases that died in a cold environment but not of hypothermia did not show the typical glomerular staining pattern. In conclusion, specific staining of podocyte nuclei seems to be a reliable marker of hypothermia.

  • 62.
    Hedlund, Linda
    Örebro University, School of Health and Medical Sciences.
    Förekomsten av Arginine Catabolic Mobile Element hos samhällsförvärade Meticillin Resistenta Staphylococcus aureus är kopplat till en specifik genetisk bakgrund2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 63.
    Hedman, Emma
    Örebro University, School of Health Sciences.
    Utvärdering av kvantitativ analys för P-etanol, P-paracetamol och P-vankomycin på kemiinstrumenten Vitros 5.1 FS och Advia XPT2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 64.
    Hedman, Emma
    Örebro University, School of Health Sciences.
    Utvärdering av kvantitativ analys för P-etanol, P-paracetamol och P-vankomycin på kemiinstrumenten Vitros 5.1 FS och Advia XPT2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 65.
    Helgesson, Madeleine
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Sekvensering av Hemoglobin Tacoma: optimering av PCR och pyrosekvensering2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 66.
    Henriksson, Lilian
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Evaluation of the Quality of Abdominla Computed Tomography Images Reconstructed with Iterative Reconstruction Technique2012Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
  • 67.
    Herrmann, Björn
    et al.
    Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
    Eden, Desiree
    Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
    Hadad, Ronza
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Örebro University Hospital, Örebro, Sweden.
    Christerson, Linus
    Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
    Loré, Britta
    Department of Clinical Microbiology, Falu Lasarett, Falun, Sweden.
    Österlund, Anders
    Communicable Disease Prevention and Control, Sunderby Hospital, Luleå, Sweden.
    Larsson, Inger
    Department of Clinical Microbiology, Sunderby Hospital, Luleå, Sweden.
    Sylvan, Staffan
    Department of Communicable Diseases Control and Prevention, Uppsala County Council, Uppsala, Sweden.
    Fredlund, Hans
    Örebro University Hospital. WHO Collaborating Centre for Gonorrhoea and Other STIs , Örebro University Hospital, Örebro, Sweden.
    Unemo, Magnus
    Örebro University Hospital. WHO Collaborating Centre for Gonorrhoea and Other STIs, Örebro University Hospital, Örebro, Sweden.
    Prevalence Trends of the New Variant of Chlamydia trachomatis in Four Counties of Sweden in 2007-20112012In: Sexually Transmitted Diseases, ISSN 0148-5717, E-ISSN 1537-4521, Vol. 39, no 8, p. 648-650Article in journal (Refereed)
    Abstract [en]

    A new variant of Chlamydia trachomatis (nvCT) was discovered in Sweden in 2006, and it could not be detected by diagnostic systems from Abbott and Roche, whereas the third system used, from Becton Dickinson (BD), detects nvCT. We analyzed 3648 samples from 2 counties that used Roche and 2 counties that used BD methods from 2007 to 2011. After implementation of a Roche method that detects nvCT, its proportion has decreased and converged in the 4 counties but are still at different levels in Roche and BD counties. Future studies are needed to see if nvCT will decline further.

  • 68.
    Hillver, Marie
    Örebro University, School of Health and Medical Sciences.
    Kroppspositionens påverkan på spirometrisundersökning samt jämförelse mellan två flödesmätande spirometrar2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 69.
    Huopaniemi, Ilkka
    et al.
    School of Science and Technology, Department of Information and Computer Science, Aalto University, Espoo, Finland; Helsinki Institute for Information Techology HIIT, Helsinki, Finland.
    Suvitaival, Tommi
    School of Science and Technology, Department of Information and Computer Science, Aalto University, Espoo, Finland; Helsinki Institute for Information Techology HIIT, Helsinki, Finland.
    Nikkilä, Janne
    School of Science and Technology, Department of Information and Computer Science, Aalto University, Espoo, Finland; Helsinki Institute for Information Techology HIIT, Helsinki, Finland; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
    Oresic, Matej
    Örebro University, School of Medical Sciences. VTT Technical Research Centre of Finland, Espoo, Finland.
    Kaski, Samuel
    School of Science and Technology, Department of Information and Computer Science, Aalto University, Espoo, Finland; Helsinki Institute for Information Techology HIIT, Helsinki, Finland.
    Multivariate multi-way analysis of multi-source data2010In: Bioinformatics, ISSN 1367-4803, E-ISSN 1367-4811, Vol. 26, no 12, p. i391-i398Article in journal (Refereed)
    Abstract [en]

    MOTIVATION: Analysis of variance (ANOVA)-type methods are the default tool for the analysis of data with multiple covariates. These tools have been generalized to the multivariate analysis of high-throughput biological datasets, where the main challenge is the problem of small sample size and high dimensionality. However, the existing multi-way analysis methods are not designed for the currently increasingly important experiments where data is obtained from multiple sources. Common examples of such settings include integrated analysis of metabolic and gene expression profiles, or metabolic profiles from several tissues in our case, in a controlled multi-way experimental setup where disease status, medical treatment, gender and time-series are usual covariates.

    RESULTS: We extend the applicability area of multivariate, multi-way ANOVA-type methods to multi-source cases by introducing a novel Bayesian model. The method is capable of finding covariate-related dependencies between the sources. It assumes the measurements consist of groups of similarly behaving variables, and estimates the multivariate covariate effects and their interaction effects for the discovered groups of variables. In particular, the method partitions the effects to those shared between the sources and to source-specific ones. The method is specifically designed for datasets with small sample sizes and high dimensionality. We apply the method to a lipidomics dataset from a lung cancer study with two-way experimental setup, where measurements from several tissues with mostly distinct lipids have been taken. The method is also directly applicable to gene expression and proteomics.

    AVAILABILITY: An R-implementation is available at http://www.cis.hut.fi/projects/mi/software/multiWayCCA/.

  • 70.
    Huskic, Merjema
    Örebro University, School of Health Sciences.
    Resistensbestämning avvankomycin medbuljongspädningsmetod2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 71.
    Hussain, Rashida
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Cell responses in infected and cystic fibrosis respiratory epithelium2014Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Respiratory Epithelium. Örebro Studies in Medicine 99. Cystic fibrosis (CF) is caused by a mutation in a cAMP-activated chloride (Cl-) channel (CFTR). Mortality and morbidity in CF is mainly due to the deregulated responses of the airway epithelial cells. The purpose of the thesis was to investigate the behaviour of the airway epithelial cells that are involved in maintaining the homeostasis in the airways.

    Nasal brush biopsies obtained from anesthetized human nasal mucosa can be an easy source to establish primary epithelial cell lines (Paper I). We found that CF and non CF cellular models cannot fully show the relation between CFTR and the phenotypic differences between CF and healthy cells (Paper II). The possibility to correct the Cl- transport defect in CF by the use of stable NOdonors, and ambroxol was investigated. NO-donors stimulated Cl- efflux, and decreased ENaC mRNA expression in CFBE cells (Paper III), while ambroxol increased Cl- efflux from CFBE cells, and showed a positive effect on the biosynthesis of CFTR (Paper IV). This suggests that these substances may be a potentially interesting group of compounds for the treatment of CF. Increased levels of IL-6 and IL-8 upon infection in CF cells can increase the susceptibility of P. aeruginosa infected CF cells to apoptosis and/or internalization of these bacteria in CF cells and hence, may have important roles in the pathology of P. aeruginosa infection in CF airways. If internalization is beneficial for the host then glucocorticoids (GCs) are not beneficial for the treatment of CF patients. However, GCs may improve airway hydration. Whether the benefits of GC treatment outweigh the negative effects is questionable, and further clinical studies need to be carried out (Paper V). The neonatal isolates S. epidermidis 94B080 and S. aureus 90B083 can modulate CFTR and ENaC expression in airway epithelial cells, which may disturb the ion transport in the respiratory epithelium upon bacterial exposure. Airway epithelial cells also show excessive inflammatory responses to these bacteria, which means that these bacteria may induce pulmonary inflammation (Paper VI).

    List of papers
    1. Isolation and culture of primary human nasal epithelial cells from anesthetized nasal epithelia
    Open this publication in new window or tab >>Isolation and culture of primary human nasal epithelial cells from anesthetized nasal epithelia
    2014 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, E-ISSN 1651-2251, Vol. 134, no 3, p. 296-299Article in journal (Refereed) Published
    Abstract [en]

    Conclusion: Using a local anesthetic agent before obtaining nasal biopsies by nasal brushing makes the sampling procedure smooth, avoids lacrimation, nasal itching/irritation, and/or sneezing and provides enough viable cells to establish primary cultures.

    Objectives: To examine the use of local anesthesia to avoid the irritation experienced by the subject when nasal biopsies are obtained by nasal brushing in order to culture viable nasal epithelial cells.

    Methods: Nasal epithelial cells were collected from the mid-part of the inferior turbinate of healthy volunteers by brushing with interdental brushes, after spraying a topical anesthetic on the nasal mucosa. Immunocytochemistry was performed to assess the purity of epithelial cells.

    Results: Cell samples ranging from 1.16 x 10(5) to 3.06 x 10(5) cells/per sample were obtained. Of 11 samples, 7 formed confluent cultures, while the remaining 4 samples showed only patches of epithelial cells. Neither fungal nor bacterial contamination posed a problem. Immunocytochemistry of the cytospin slides confirmed the presence of epithelial cells in the cultures. No adverse effects were experienced by the volunteers.

    Place, publisher, year, edition, pages
    London, United Kingdom: Informa Healthcare, 2014
    Keywords
    Nasal brush biopsy, airway epithelia, lidocaine
    National Category
    Medical and Health Sciences Otorhinolaryngology
    Research subject
    Medicine
    Identifiers
    urn:nbn:se:oru:diva-34502 (URN)10.3109/00016489.2013.859396 (DOI)000331829700014 ()24359095 (PubMedID)2-s2.0-84894384458 (Scopus ID)
    Funder
    Swedish Heart Lung FoundationSwedish Research Council
    Available from: 2014-03-31 Created: 2014-03-31 Last updated: 2018-06-05Bibliographically approved
    2. ENaC, iNOS, mucins expression, and wound healing in cystic fibrosis airway epithelial and submucosal cells
    Open this publication in new window or tab >>ENaC, iNOS, mucins expression, and wound healing in cystic fibrosis airway epithelial and submucosal cells
    2013 (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    with endogenous wild-type cystic fibrosis transmembrane conductance regulator (CFTR), CFBE cells with mutated ΔF508-CFTR, corrected CFBE cells overexpressing CFTR, CFSME (CF submucosal) and Calu-3 (non-CF submucosal) cells with respect to the epithelial sodium channel (ENaC), inducible NO synthase (iNOS), and mucins (MUC) (studied by quantitative Real-Time-Polymerase Chain Reaction, qRT-PCR, and Western blot), and wound healing.

    CFBE cells had significantly more expression of b- and g-ENaC mRNA and of b-ENaC protein than 16HBE cells. Compared to corrected CFBE cells, CFBE cells had increased mRNA expression of all ENaC subunits and b-ENaC protein. For ENaC, the CFSME/Calu-3 mRNA ratio was very low and contradictory to the ENaC upregulation in CF cells. CFBE cells showed decreased expression of iNOS at both mRNA and protein levels compared to 16HBE cells and only at the mRNA level compared to corrected CFBE cells. CFSME cells showed expression of iNOS whereas Calu-3 cells did not. Higher expression of MUC2 and MUC5B was found in corrected CFBE cells compared to CFBE cells. Wound healing in CFBE cells was delayed compared to corrected CFBE cells, but not to 16HBE cells, and in CFSME cells compared to Calu-3 cells.

    Our data suggest CFSME as an inappropriate CF cell model for Calu-3 cells, and provide only partial support for the theory that the differences (in ENaC, iNOS, mucins, and wound healing) between these cell lines are associated to the presence of CFTR in the bronchial airway epithelial cells.

    Keywords
    Cystic fibrosis, CFTR, ENaC, iNOS, mucin, wound healing, CFTR inh-172
    National Category
    Medical and Health Sciences
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-32765 (URN)
    Funder
    Swedish Heart Lung Foundation
    Available from: 2013-12-13 Created: 2013-12-13 Last updated: 2018-04-23Bibliographically approved
    3. The effect of NO-donors on chloride efflux, intracellular Ca2+ concentration and mRNA expression of CFTR and ENaC in cystic fibrosis airway epithelial cells
    Open this publication in new window or tab >>The effect of NO-donors on chloride efflux, intracellular Ca2+ concentration and mRNA expression of CFTR and ENaC in cystic fibrosis airway epithelial cells
    Show others...
    2013 (English)In: Experimental and molecular pathology (Print), ISSN 0014-4800, E-ISSN 1096-0945, Vol. 94, no 3, p. 474-480Article in journal (Refereed) Published
    Abstract [en]

    Since previous studies showed that the endogenous bronchodilator, S nitrosglutathione (GSNO), caused amarked increase in CFTR-mediated chloride (Cl−) efflux and improved the trafficking of CFTR to the plasmamembrane, and that also the nitric oxide (NO)-donor GEA3162 had a similar, but smaller, effect on Cl− efflux, itwas investigatedwhether the NO-donor properties of GSNOwere relevant for its effect on Cl− efflux fromairwayepithelial cells. Hence, the effect of a number of other NO-donors, sodium nitroprusside (SNP), S-nitroso-Nacetyl-DL-penicillamine (SNAP), diethylenetriamine/nitric oxide adduct (DETA-NO), and diethylenetriamine/nitricoxide adduct (DEA-NONOate) on Cl− efflux from CFBE (ΔF508/ΔF508-CFTR) airway epithelial cells was tested.Cl− efflux was determined using the fluorescent N-(ethoxycarbonylmethyl)-6-methoxyquinoliniu bromide(MQAE)-technique. Possible changes in the intracellular Ca2+ concentration were tested by the fluorescent fluo-4method in a confocal microscope system. Like previously with GSNO, after 4 h incubation with the NO-donor, anincreased Cl− efflux was found (in the order SNAP > DETA-NO > SNP). The effect of DEA-NONOate on Cl− effluxwas not significant, and the compound may have (unspecific) deleterious effects on the cells. Again, as withGSNO, after a short (5 min) incubation, SNP had no significant effect on Cl− efflux. None of the NO-donors thathad a significant effect on Cl− efflux caused significant changes in the intracellular Ca2+ concentration. After 4 hpreincubation, SNP caused a significant increase in the mRNA expression of CFTR. SNAP and DEA-NONOatedecreased the mRNA expression of all ENaC subunits significantly. DETA-NO caused a significant decrease only inα-ENaC expression. After a short preincubation, none of the NO-donors had a significant effect, neither on theexpression of CFTR, nor on that of the ENaC subunits in the presence and absence of L-cysteine. It can be concludedthat the effect of GSNO on Cl−efflux is, at least in part, due to its properties as an NO-donor, and the effect is likely tobe mediated by CFTR, not by Ca2+-activated Cl− channels.

    Place, publisher, year, edition, pages
    Maryland Heights, USA: Elsevier, 2013
    Keywords
    Nitric oxide donors, Cystic fibrosis, Airway epithelium, CFTR, ENaC, Calcium
    National Category
    Medical and Health Sciences Cell Biology
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-32767 (URN)10.1016/j.yexmp.2013.03.003 (DOI)000319535900008 ()23523754 (PubMedID)2-s2.0-84876344605 (Scopus ID)
    Funder
    Swedish Heart Lung FoundationNIH (National Institute of Health)Swedish Research Council
    Available from: 2013-12-13 Created: 2013-12-13 Last updated: 2017-12-06Bibliographically approved
    4. The effect of ambroxol on chloride transport, CFTR and ENaC in cysticfibrosis airway epithelial cells
    Open this publication in new window or tab >>The effect of ambroxol on chloride transport, CFTR and ENaC in cysticfibrosis airway epithelial cells
    Show others...
    2013 (English)In: Cell Biology International, ISSN 1065-6995, E-ISSN 1095-8355, Vol. 37, no 11, p. 1149-1156Article in journal (Refereed) Published
    Abstract [en]

    Ambroxol, a mucokinetic anti-inflammatory drug, has been used for treatment of cystic fibrosis (CF). The respiratoryepitheliumis covered by the airway surface liquid (ASL), the thickness and composition of which is determined by Cl efflux viathe cystic fibrosis transmembrane conductance regulator (CFTR) and Naþ influx via the epithelial Naþ channel (ENaC). In cellsexpressing wt-CFTR, ambroxol increased the Cl- conductance, but not the bicarbonate conductance of the CFTR channels.Weinvestigated whether treatment with ambroxol enhances chloride transport and/or CFTR and ENaC expression in CF airwayepithelial cells (CFBE) cells. CFBE cells were treated with 100 mM ambroxol for 2, 4 or 8 h. mRNA expression for CFTR andENaC subunits was analysed by real-time polymerase chain reaction (RT-PCR); protein expression was measured by Westernblot. The effect of ambroxol on Cl− transport was measured by Cl− efflux measurements with a fluorescent chloride probe.Ambroxol significantly stimulated Cl− efflux from CFBE cells (a sixfold increase after 8 h treatment), and enhanced theexpression of the mRNA of CFTR and a-ENaC, and of the CFTR protein. No significant difference was observed in b-ENaCafter exposure to ambroxol, whereasmRNA expression of g-ENaC was reduced. No significant effects of ambroxol on the ENaCsubunits were observed by Western blot. Ambroxol did not significantly affect the intracellular Ca2+ concentration.Upregulation of CFTR and enhanced Cl efflux after ambroxol treatment should promote transepithelial ion and watertransport, which may improve hydration of the mucus, and therefore be beneficial to CF-patients.

    Place, publisher, year, edition, pages
    Hoboken, USA: Wiley-Blackwell, 2013
    Keywords
    Airway epithelium, ambroxol, cystic fibrosis, Cl− efflux, CFTR; ENaC
    National Category
    Medical and Health Sciences Cell Biology
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-32769 (URN)10.1002/cbin.10146 (DOI)000325489500002 ()23765701 (PubMedID)2-s2.0-84885861654 (Scopus ID)
    External cooperation:
    Funder
    Swedish Heart Lung FoundationSwedish Research Council
    Note

    Funding Agencies:

    Swedish Science Research Council 

    Available from: 2013-12-13 Created: 2013-12-13 Last updated: 2017-12-06Bibliographically approved
    5. Effect of IL-6, IL-8 and glucocorticoids on the internalization of Pseudomonas aeruginosa (ATCC 27853) in cystic fibrosis bronchial epithelial cells
    Open this publication in new window or tab >>Effect of IL-6, IL-8 and glucocorticoids on the internalization of Pseudomonas aeruginosa (ATCC 27853) in cystic fibrosis bronchial epithelial cells
    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    Pseudomonas aeruginosa infection is common in cystic fibrosis (CF). Uptake of P. aeruginosa by the cell and the subsequent apoptosis may prevent colonization of P. aeruginosa in CF airways. CF airways have elevated levels of IL-6 and IL-8. Glucocorticoids (GCs) are anti-inflammatory but their use in CF is controversial. We studied the effect of IL- 6, IL-8 and GCs on bacterial internalization, apoptosis, and intracellular Ca2+concentration in CF bronchial epithelial (CFBE) cells and found that increased levels of IL-6 and IL-8 can increase the susceptibility of P. aeruginosa infected cells to apoptosis and/or internalization of these bacteria in CF cells. GCs decreased the extent of apoptosis in CFBE cells infected with P. aeruginosa, but may improve airway hydration by increasing the intracellular Ca2+ concentration. None of the GCs and cytokines affected apoptosis in cells not exposed to Pseudomonas. We conclude that increased levels of IL-6 and IL-8 may have important roles in the pathology of P. aeruginosa infection in CF airways. If internalization is beneficial for the host then GCs are not beneficial for the treatment of CF patients. Whether the benefits of GC treatment outweigh the negative effects is questionable, and further clinical studies need to be carried out. 

    Keywords
    cystic fibrosis, Pseudomonas aeruginosa, internalization, apoptosis, glucocorticoids.
    National Category
    Pharmacology and Toxicology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
    Research subject
    Biomedicine
    Identifiers
    urn:nbn:se:oru:diva-35865 (URN)
    Available from: 2014-08-05 Created: 2014-08-05 Last updated: 2018-01-11Bibliographically approved
    6. Modulation of ENaC, CFTR, and iNOS expression in bronchial epithelial cells after stimulation with Staphylococcus epidermidis (94B080) and Staphylococcus aureus (90B083)
    Open this publication in new window or tab >>Modulation of ENaC, CFTR, and iNOS expression in bronchial epithelial cells after stimulation with Staphylococcus epidermidis (94B080) and Staphylococcus aureus (90B083)
    2013 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 121, no 9, p. 814-826Article in journal (Refereed) Published
    Abstract [en]

    Bacteria affect the respiratory epithelium, which is covered by airway surface liquid (ASL) and mucus. Ion concentrations in the ASL are determined by the cystic fibrosis transmembrane conductance regulator (CFTR) and the epithelial Na+ channel (ENaC). Neonatal sepsis is a major risk factor for subsequent pulmonary disease in preterm newborns. Predominating are coagulase-negative staphylococci (e.g., Staphylococccus epidermidis and Staphylococccus aureus). The aim of this study was to investigate modulation of CFTR, ENaC, mucins, proinflammatory cytokines, and inducible nitric oxide synthase (iNOS) in respiratory epithelial cells after S. epidermidis 94B080 and S. aureus 90B083 exposure. Bronchial epithelial cells were incubated with S. epidermidis 94B080 and S. aureus 90B083 (neonatal blood isolates) for 1-36h. Expression of CFTR, ENaC, iNOS, and mucins was analyzed by real-time PCR and Western blotting. Release of cytokines was analyzed by ELISA, and production of NO by the Griess assay. Expression of CFTR significantly decreased after 36h incubation with S. epidermidis and more prominently with S. aureus, whereas S. epidermidis caused a significant increase in the expression of - and -ENaC. Expression of iNOS increased, but NO was not detected. Both staphylococci caused a decrease in the intracellular Ca2+ concentration. S. aureus induced increased secretion of IL-6, IL-8, and transforming nuclear factor (TNF)- in a time-dependent manner as compared with S. epidermidis. In conclusion, expression of ENaC, CFTR, and iNOS is modulated by exposure to S. aureus 90B083 and S. epidermidis 94B080. S. aureus is more potent in causing release of IL-6, IL-8, and TNF- by bronchial epithelial cells as compared with S. epidermidis. The mRNA expression for the mucus proteins MUC2, MUC5AC, and MUC5B could not be measured, neither in the presence nor in the absence of bacteria.

    Place, publisher, year, edition, pages
    Hoboken, USA: Wiley-Blackwell, 2013
    Keywords
    Airway epithelium, Staphylococcus aureus 90B083, Staphylococcus epidermidis 94B080, CFTR, ENaC, iNOS, cytokines
    National Category
    Medical and Health Sciences Immunology
    Research subject
    Medicine
    Identifiers
    urn:nbn:se:oru:diva-30514 (URN)10.1111/apm.12138 (DOI)000322757000003 ()23879620 (PubMedID)2-s2.0-84881558007 (Scopus ID)
    Note

    Funding Agencies:

    Nyckelfonden of Örebro University Hospital 

    Swedish Science Research Council 

    Swedish Heart Lung Foundation 

    Available from: 2013-08-30 Created: 2013-08-30 Last updated: 2018-05-21Bibliographically approved
  • 72.
    Idahir. M, Eman
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Effect of Porphyromonas gingivalis and TNF-α on the Expression of IL-1β in Human Umbilical Vein Endothelial Cells2012Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
  • 73.
    Ivanova, Kateryna
    Örebro University, School of Health Sciences.
    Bakgrundskorrigeringens inverkan på den relativt beräknade njurfunktionen vid 99mTc-DMSA skintigrafi2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 74.
    Jansson, Arvid
    Örebro University, School of Health Sciences.
    Skillnader i uppmätta vänsterkammarmått hos män: Jämförelse mellan leg. biomedicinska analytiker och biomedicinska analytikerstudenter2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 75.
    Jesperson Mora, Anna
    Örebro University, School of Health Sciences.
    Hur påverkar åldern på ultraljudsmaskinen bestämningen av vänster kammares ejektionsfraktion?: En jämförelsestudie mellan två ultraljudsmaskiner med en åldersskillnad på 10 år.2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 76.
    Johansson, Elin
    Örebro University, School of Health Sciences.
    Betydelsen av fibroblasters interaktionmed omgivande substrat för genuttryck avfibrosmarkörer2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 77.
    Johansson, Emma
    Örebro University, School of Health and Medical Sciences.
    Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000-20072011Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
  • 78.
    Johansson, Joakim
    Örebro University, School of Health Sciences.
    Verifiering av två nya metoder för urin-IgG immunokemianalys på Siemens Advia 18002019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 79.
    Johansson, Klara
    Örebro University, School of Health Sciences.
    Stress vid fallskärmshopp påverkar inte tarmbarriären2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 80.
    Johansson, Madelene
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    The role of the P2Y 2-receptor in uropathogenic Escherichia coli evoked cytokine from release from uroepithelial cells2012Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 81.
    Jonsson, Jonny
    Örebro University, Department of Clinical Medicine.
    Hur säker är ekokardiografi för mätning av LVEF?: Jämförelse mot gated SPECT MPS2007Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    How correct is LVEF calculated by echocardiography?

    Comparison with gated SPECT.

    LVEF is an important measurement for evaluating prognosis and treatment at heart diseases.

    Aim: The aim was to analyse echocardiography and four different models at echocardiography (MRR, Teichholtz, Simpsons biplan and visual estimation) for measuring LVEF value and to investigate if echocardiography is dependent of the echocardiographer. The reference method was gated SPECT (g-SPECT).

    Patients/method: 133 patients who had undergone echocardiography and g-SPECT at the university hospital of Örebro within 202 days of each other were analysed retrospectively. The correlation (r), significance (p), difference and agreement between the two methods where calculated. Agreement were visualised by Bland-Altman plot. A p-value of 0,05 were considered significant and an agreement of < 20 LVEF % were considered acceptable.

    Results: The agreement and correlation between echocardiography and g-SPECT was acceptable (r=0,779, p<0,001, limits of agreement + 20,8; - 11,6).

    The agreement and correlation for the calculation models Simpsons biplan method and visual estimation on echocardiography were acceptable (r=0,86 and 0,79, p<0,001, limits of agreement + 19,1;-5,7 and + 19,5;-11,7).

    The agreement and correlation for the calculation models MRR and Teichholtz method on echocardiography was not acceptable (r=0,46 and 0,47, p + 30,1;-18,9 and + 23,7;-11,1). Echocardiography was not dependent on the echocardiographer (p=0,419).

    Conclusion: Echocardiography has an acceptable agreement with the reference method but the agreement is different for the different models. The results must be viewed critically since the reference method contains multiple sources of errors.

  • 82.
    Juma, Mahmoud
    Örebro University, School of Health and Medical Sciences.
    Analys av leukocytinnehållet i framställd plasmakomponent för transfusion2011Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
  • 83.
    Jönsson, Agnez
    et al.
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Jacobsson, Susanne
    Örebro University, School of Medical Sciences. Örebro University Hospital. WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology.
    Foerster, Sunniva
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Cole, Michelle J.
    Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK.
    Unemo, Magnus
    Örebro University, School of Medical Sciences. Örebro University Hospital. WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology.
    Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae2018In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 126, no 10, p. 822-827Article in journal (Refereed)
    Abstract [en]

    For Neisseria gonorrhoeae susceptibility testing, Etest, comparable to agar dilution, is frequently used. In recent years, newer MIC gradient strip tests have been commercialized. However, these tests have not been appropriately evaluated for gonococci. We evaluated the sensitivity, specificity, accuracy, quality, availability of antimicrobials and cost of the MIC Test Strip (Liofilchem), M.I.C.Evaluator (Oxoid) and Ezy MIC Strip (HiMedia), compared to the reference Etest (bioMérieux), for gonococcal susceptibility testing. The MICs of eight antimicrobials in 103 gonococcal international reference strains (n = 29) and clinical isolates (n = 74) were examined. Coefficient of determination (R2), complete agreement, essential agreement, SIR categorical agreement, sensitivity, specificity and accuracy were calculated. R2 of the MICs for the antimicrobials ranged between 0.674–0.996, 0.617–0.993, and 0.643–0.994 for the MIC Test Strip, M.I.C.Evaluator strips and Ezy MIC Strips respectively. The essential agreement (SIR categorical agreement) was 99.6% (88.6%), 100% (87.1%) and 93.0% (83.1%) respectively. M.I.C.Evaluator strips for gonococcal key antimicrobials were lacking and the Ezy MIC Strips showed an inconsistent accuracy, quality and some strips were contaminated. The Liofilchem MIC Test Strips had limitations, but might be relatively accurate alternatives to Etest for gonococci. Strict quality assurance (at manufacturing and testing laboratory), including quality controls, are required.

  • 84.
    Karefylakis, Christos
    et al.
    Örebro University, School of Medical Sciences. Department of Endocrinology.
    Pettersson-Pablo, Paul
    Örebro University, School of Medical Sciences. Department of Clinical Chemistry, Örebro University Hospital, Örebro, Sweden.
    Särnblad, Stefan
    Örebro University, School of Medical Sciences. Department of Pediatrics.
    Rask, Eva
    Department of Endocrinology, School of Medical Sciences, Örebro University, Örebro, Sweden.
    Bitar, Manar
    Department of Clinical Chemistry, Örebro University Hospital, Örebro, Sweden.
    Magnusson, Anders
    Clinical Epidemiology and Biostatistics, School of Medical Sciences, Örebro University, Örebro, Sweden.
    Eriksson, Clas-Göran
    Department of Clinical Chemistry, Örebro University Hospital, Örebro, Sweden.
    Vitamin D C3 epimer in a mid-Swedish region: Analytical measurement and epidemiology2018In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 478, p. 182-187Article in journal (Refereed)
    Abstract [en]

    Background: The discovery of an epimeric form of 25(OH)D3 may complicate the interpretation of vitamin Dstatus. The aim of this study was to examine the prevalence and determinants of 25-hydroxy-3-epi-vitamin D3 (3- epi-25(OH)D3) in a mid-Swedish region and to investigate how the measurement of 3-epi-25(OH)D3 would affect the assessment of vitamin D status using current thresholds.

    Methods: We conducted a cross-sectional study of 8286 in- and outpatients in primary as well as secondary care settings. Plasma 25(OH)D, 25(OH)D2, 25(OH)D3 and 3-epi-25(OH)D3 were measured using High Pressure Liquid Chromatography – Tandem Mass Spectrometry (HPLC – MS/MS). The relative 3-epi-25(OH)D3 contribution was calculated as a percentage of the total 25(OH)D3. Blood samples were collected between March 2014 and July 2015 providing a seasonal aspect to the results.

    Results: 3-epi-25(OH)D3was detected in 635 cases (7.7% of all subjects), and the mean concentration was8.4 ± 3.5 nmol/L. 3-epi-25(OH)D3correlated significantly with 25(OH)D3(r =0.38, p < 0.001).A multivariateanalysis among the detected showed that male gender and winter season were independently associatedwith higher 3-epi-25(OH)D3/25(OH)D3percentage ratio (R2=0.044). Infants and children had a significantlyhigher detection rate compared to the reference age category (18–45 years) as well as those who were testedduring the summer season.

    Conclusions: We report findings from the first epidemiologic study of 3-epi-25(OH)D3 conducted in Sweden, based on a large population sample. 3-epi-25(OH)D3 was detected in 7.7% of the study population and the mean concentration was 8.4 nmol/L. The quantification of 3-epi-25(OH)D3 would not significantly influence the clinical interpretation of vitamin D levels. Additional studies are needed to understand the metabolic pathway and the possible physiological functions of this metabolite.

  • 85.
    Karlsson, Mats G.
    et al.
    Department of Otorhinolaryngology, Örebro Medical Center Hospital, Örebro, Sweden; Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden.
    Davidsson, Åke
    Department of Otorhinolaryngology, Örebro Medical Center Hospital, Örebro, Sweden; Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden.
    Hellquist, H. B.
    Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden.
    Quantitative computerized image analysis of immunostained lymphocytes: A methodological approach1994In: Pathology, Research and Practice, ISSN 0344-0338, E-ISSN 1618-0631, Vol. 190, no 8, p. 799-807Article in journal (Refereed)
    Abstract [en]

    A methodological approach by computerized image analysis to quantify immunostained objects in histological sections is described. We have investigated antibodies against CD4, CD8, CD20, CD23 and CD25 in frozen sections of human nasal mucosa; however, the methodology of standardization is of general validity. The study was designed particularly to investigate the following points: 1) light intensity, 2) the grey level for counter staining intensity, 3) the grey level threshold value for positive objects, 4) the minimal acceptable size of a positive object, 5) the influence of the brightness of the light on both the number and the area of objects. Furthermore, random sampling and determination of 6) the area per section, and 7) the number of histological sections to be measured per biopsy. Finally, a study of reproducibility of immunostaining intensity was performed. The influence of the different parameters mentioned above was studied and the values (eg. threshold value) for our particular setting of microscope, image analysis equipment, computer software etc, were defined. The method was then tested for intra- and interindividual variation which was found to be less than 5%. Correlation analysis of the reproducibility gave coefficients of correlation of 0.99, both concerning number of immunopositive objects and immunopositive area. We emphasize the importance of a highly standardized methodology if the numeric data obtained from computer assisted image analysis are to be more accurate than semiquantitative assessments by experienced observers. With a thorough standardization as described in this method it is possible to obtain numeric values, and data with low deviations, which are two obvious and important advantages.

  • 86.
    Koosam, Vijay Kumar
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Ocular Accommodation with Regards to Age and Gender2012Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
  • 87.
    Kucska, Julia
    Örebro University, School of Health Sciences.
    Analys av interleukin 6 i plasma och trombocytrik plasma hos friska individer2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 88.
    Kumawat, Ashok Kumar
    et al.
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Elgbratt, Kristina
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Tysk, Curt
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Bohr, Johan
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Reduced T cell receptor excision circle (TREC) levels in the colonic mucosa of microscopic colitis patients indicate local proliferation rather than homing of peripheral lymphocytes to the inflamed mucosaManuscript (preprint) (Other academic)
    Abstract [en]

    Aims: Dysregulated T cell responses in the intestine may lead to chronic bowel inflammation such as collagenous colitis (CC) and lymphocytic colitis (LC), together known as microscopic colitis (MC). Having demonstrated increased local T cell responses in the intestinal mucosa of MC patients, we investigated the recent thymic emigrants by measuring T cell receptor excision circle (TREC) levels in the colonic mucosa of CC and LC patients.

    Methods: Mucosal biopsies from CC (n=8), LC (n=5), and CC or LC patients in histopathological remission, (CC-HR, n=3), (LC-HR, n=6), non-inflamed diarrhoea patients (n=17) and controls (n=10) were analysed for TRECs expression by real time PCR.

    Results: The median TREC levels were lower in both CC and LC patients as well as in LCHR patients compared to controls. In contrast to MC patients, non-inflamed diarrhoea patients presented with enhanced TREC levels compared to controls. None of the recorded differences did however reach statistical significance. No differences were observed in median TREC levels in either CC-HR or LC-HR patients compared to active CC and LC patients. A trend towards increased relative expression of CD3 was noted in all MC subgroups examined; and reached statistical significance in LC patients compared to controls. LC patients had ignificantly increased CD3 mRNA levels also compared to CC, CC-HR, LC-HR and non-inflamed iarrhoea patients.

    Conclusions: Reduced TRECs level in the colonic mucosa, together with our previously demonstrated enhanced expression of Ki67+ T cells, suggest local expansion of resident T lymphocytes in the inflamed mucosa of MC patients.

  • 89.
    Kumawat, Ashok Kumar
    et al.
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Tysk, Curt
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Bohr, Johan
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Hultgren, Olof
    Örebro University, School of Medicine, Örebro University, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro University, School of Medicine, Örebro University, Sweden.
    An in vitro model for analysis of the impact of the colonic milieu in collagenous colitis patients on peripheral T lymphocyte activation and differentiationManuscript (preprint) (Other academic)
    Abstract [en]

    Background: Soluble factors released by intestinal mucosal cells contribute to immune homeostasis in the gut. This is the first study to investigate the role of soluble factors from the intestinal mucosa of collagenous colitis (CC) patients in the regulation of effector T cells using a novel system that mimics the in vivo exposure of newly recruited peripheral blood T cells to soluble factors derived from the colonic milieu of normal individuals and inflamed CC patient mucosa.

    Methods: Denuded biopsies (DNB) and isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients and non-inflamed controls were cultured to collect conditioned medium (CM). Enriched peripheral blood CD4+ T cells from healthy donors were polyclonally activated in the absence or presence of CM from CC patients and controls. Proliferation, as well as secretion of IL-1β IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α was analysed the latter with Luminex® analysis.

    Results: Peripheral CD4+ T cells exposed to CM from the colonic mucosa demonstrated reduced proliferation. This inhibition was less pronounced with DNB-CM derived from CC patients compared to non-inflamed control mucosa. In contrast, LPMC-CM from non-inflamed controls inhibited T-cell proliferation less than LPMC-CM from CC patients. Both DNB-CM and LPMC-CM from CC patients induced more or less increased production of the proinflammatory cytokines IFN-γ, IL-17A, IL-6 and TNF-α as well as the anti-inflammatory cytokines IL-4 and IL-10 from peripheral CD4+ T cells compared to non-inflamed controls. In contrast, IL-1β production by peripheral T cells showed mixed results – it was either increased or reduced in the presence of both DNB and LPMC-CM from CC patients compared to noninflamed controls with different blood donors and different concentrations.

    Conclusion: Our preliminary data indicates reduced inhibition of proliferation of peripheral CD4+ T cells in the presence of mucosa-derived soluble factors from CC patients compared to controls. In addition, increased production of both inflammatory and anti-inflammatory cytokines by peripheral CD4+ T cells was recorded in the presence of soluble factors from the colonic mucosa of CC patients compared to controls. This model can be valuable in evaluating the effect(s) of existing and new drugs on T cell differentiation in the intestinal mucosa.

  • 90.
    Kälvegren, Hanna
    et al.
    Örebro University, School of Health and Medical Sciences.
    Jonsson, Simon
    Örebro University, School of Health and Medical Sciences.
    Jonasson, Lena
    Örebro University, School of Health and Medical Sciences.
    Release of matrix metalloproteinases-1 and-2, but not-9, from activated platelets measured by enzyme-linked immunosorbent assay2011In: Platelets, ISSN 0953-7104, E-ISSN 1369-1635, Vol. 22, no 8, p. 572-578Article in journal (Refereed)
    Abstract [en]

    Matrix metalloproteinases (MMPs), in particular MMP-9, have been introduced as novel biomarkers in coronary artery disease. Activated platelets are considered to be a major source of the highly elevated levels of MMPs that are detected in serum compared to plasma. The aim of this study was to clarify if activated platelets release MMPs-1, -2 and -9 as measured by enzyme-linked immunosorbent assays (ELISA). Isolated platelets (separated by several procedures) or platelet-rich plasma (PRP) were stimulated by collagen, thrombin or the TLR2 agonist Pam(3)CSK(4). The concentrations of MMPs-1,-2 and -9 in supernatants were determined by ELISA. In addition, a MMP-9 enzyme activity assay was used as well as immunofluorescent staining of MMPs-1,-2 and 9 in platelets. Isolated platelets stimulated by collagen, thrombin or Pam(3)CSK(4) released significant amounts of MMP-1 to the supernatant measured as either pro- or total-MMP-1. However, there was no detectable release of MMP-2 or -9 from isolated platelets. Collagen-stimulated platelets in PRP released MMP-2, but not -9. Before stimulation; platelets were positive for MMPs-1 and -2, but not -9, as assessed by immunofluorescence. Acting as positive controls, neutrophils were found to release significant amounts of MMP-9. Our findings indicate that activated platelets may be a major source of MMP-1 and to a minor extent MMP-2, in peripheral blood. However, in contrast to what has been argued in previous literature, platelets appear to be only negligible contributors to circulating MMP-9.

  • 91.
    Larsson, Gabriella Lillsunde
    et al.
    Örebro University, School of Medical Sciences. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Karlsson, Mats G
    Örebro University, School of Medical Sciences. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Helenius, Gisela
    Örebro University, School of Medical Sciences. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    HPV testing of biobanked liquid-based cytology: a validation study2016In: International Journal of Biological Markers, ISSN 0393-6155, E-ISSN 1724-6008, Vol. 31, no 2, p. E218-E223Article in journal (Refereed)
    Abstract [en]

    Introduction: The aim of the study was to investigate whether biobanked liquid-based cytology (LBC) vaginal samples could be reanalyzed for the biomarkers HPV DNA and mRNA without loss of sensitivity.

    Methods: One hundred LBC samples with ASCUS or CIN1 were tested for HPV DNA and mRNA before and after biobanking. DNA analysis targeted the viral genes E6 and E7, 12 high-risk and 2 low-risk HPV types together with the human control gene HBB, using real-time PCR. The Aptima HPV assay was used for mRNA analysis of 14 high-risk HPV types.

    Results: With Aptima there was 84% agreement between results before and after biobanking. The sensitivity and specificity were 0.79 (95% CI, 0.68-0.88) and 0.94 (95% CI, 0.80-0.99), respectively. With the DNA-based method, the agreement between results was 87%, the sensitivity 0.85 (95% CI, 0.75-0.92) and the specificity 0.95 (95% CI, 0.77-1.00). Both methods presented a significant difference between positive results before and after biobanking; McNemar test: p = 0.004, p = 0.003, Cohen's kappa: 0.67 (95% CI, 0.53-0.81), 0.68 (95% CI, 0.52-0.84). Cycle threshold values for the DNA method were higher for all genotypes after biobanking, except for HPV-59. Some loss of sensitivity was seen after biobanking but the concordance between HPV detection before and after biobanking was good for both evaluated methods.

    Conclusions: Biobanking of LBC vaginal samples offers a good platform for HPV testing and could be extended to further molecular analyses. However, in order to ensure a valid test result a larger portion needs to be analyzed from the biobanked sample.

  • 92.
    Lempicka, Agnieszka Julia
    Örebro University, School of Health Sciences.
    Planering samt effektivisering vid dehydrering av små preparat2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 93.
    Lillsunde-Larsson, Gabriella
    et al.
    Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Carlsson, Jessica
    Örebro University Hospital. Department of Urology, Örebro University Hospital, Örebro, Sweden.
    Karlsson, Mats G.
    Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Helenius, Gisela
    Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
    Evaluation of HPV Genotyping Assays for Archival Clinical Samples2015In: Journal of Molecular Diagnostics, ISSN 1525-1578, E-ISSN 1943-7811, Vol. 17, no 3, p. 293-301Article in journal (Refereed)
    Abstract [en]

    Human papillomavirus (HPV) testing and genotyping of FFPE tissue samples is important in epidemiological investigations. Here, we compare four different HPV genotyping methods for use in FFPE clinical samples. Comparative testing was performed on 99 samples with a clinical suspicion of HPV. Specimens were analyzed with Anyplex II HPV28 detecting 28 genotypes using real-time PCR and melting curve analysis, CLART HPV2 detecting 35 genotypes using PCR and microarray detection, and MGP5+/6+ consensus primer system together with pyrosequencing. Results were compared to a real-time PCR reference protocol detecting 14 genotypes. In total, 68% of the samples were positive for an HPV genotype using the reference protocol and MGP5+/6+ primer system. Anyplex II HPV28 analysis and CLART HPV2 had 82% and 72% positive samples, respectively. All four methods showed good agreement when comparing the 14 genotypes included in the reference protocol. When evaluating all genotypes, the Anyplex II HPV28 assay and the CLART assay changed the status of the sample (individually or together) from negative with respect to the reference protocol to positive for either a Group 1 (n = 4) or Group 2 (n = 6) genotype. We conclude from this study that for an extended genotyping approach with a high sensitivity for FFPE specimens, both the Anyplex II HPV28 and CLART HPV2 assays are suitable alternatives despite minor intra-assay differences.

  • 94.
    Lindberg, Josefin
    Örebro University, School of Health Sciences.
    Gramklassificering och kvantifiering av bakteriellt DNA med Droplet Digital PCR för att följa behandlingseffekten hos sepsispatienter2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 95.
    Ljuca, Dzejla
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Multiplex kvantifiering av antikroppar mot Neisseria meningitidis serogrupp A, C, Y och W med hjälp av Luminex-metoden2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 96.
    Ludvigsson, Jonas F.
    et al.
    Örebro University, Department of Clinical Medicine.
    Ludvigsson, Johnny
    Ekbom, Anders
    Montgomery, Scott M.
    Örebro University, Department of Clinical Medicine.
    Celiac disease and risk of subsequent type 1 diabetes: a general population cohort study of children and adolescents2006In: Diabetes Care, ISSN 0149-5992, E-ISSN 1935-5548, Vol. 29, no 11, p. 2483-2488Article in journal (Refereed)
    Abstract [en]

    OBJECTIVE:

    Earlier studies suggest that children with type 1 diabetes are more likely to have a subsequent diagnosis of celiac disease. However, research is sparse on the risk of subsequent type 1 diabetes in individuals with celiac disease. We sought to determine the risk of subsequent type 1 diabetes diagnosed before the age of 20 years in children and adolescents with celiac disease in a national, general population-based cohort.

    RESEARCH DESIGN AND METHODS:

    We identified 9,243 children with a diagnosis of celiac disease in the Swedish national inpatient register between 1964 and 2003. We then identified five reference individuals matched at time of diagnosis for age, calendar year, sex, and county (n = 45,680). Only individuals with >1 year of follow-up after study entry (diagnosis of celiac disease) were included in the analyses.

    RESULTS:

    Celiac disease was associated with a statistically significantly increased risk of subsequent type 1 diabetes before age 20 years (hazard ratio 2.4 [95% CI 1.9-3.0], P < 0.001). This risk increase was seen regardless of whether celiac disease was first diagnosed between 0 and 2 (2.2 [1.7-2.9], P < 0.001) or 3 and 20 (3.4 [1.9-6.1], P < 0.001) years of age. Individuals with prior celiac disease were also at increased risk of ketoacidosis or diabetic coma before the age of 20 years (2.3 [1.4-3.9], P = 0.001).

    CONCLUSIONS:

    Children with celiac disease are at increased risk of subsequent type 1 diabetes. This risk increase is low considering that 95% of individuals with celiac disease are HLA-DQ2 positive.

  • 97.
    Lundberg, Camilla
    Örebro University, School of Health and Medical Sciences.
    Metod för att analysera antalet T-lymfocyter innehållande T cell receptor rearrangement excision circles i tarmbiopsier2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 98.
    Lundberg, Joni
    Örebro University, School of Health and Medical Sciences.
    Investigation of the mechanism of heme oxygenase-1 induced protection of HL-1 cardiomyocyte cells2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 99.
    Matsubara, Kiri
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Kalcium- och magnesiumkoncentration i hemodialysvätska: validering av en ny analysmetod2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 100.
    Mazgaleva Ananiev, Ekaterina
    Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
    Polymorfismen rs1205 i CRP genen och dess betydelse för C-reaktivt protein-värdet hos friska unga individer2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
1234 51 - 100 of 151
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