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  • 51.
    Rangel, Ignacio
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Ganda Mall, John Peter
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Roger, Willén
    Department of Pathology and Cytology, Uppsala University Hospital, Uppsala, Sweden.
    Sjöberg, Fei
    Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Göteborg, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för medicinska vetenskaper.
    Degree of colitis correlates with microbial composition and cytokine responses in colon and caecum of Gαi2-deficient mice2016Inngår i: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 92, nr 7, artikkel-id fiw098Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    An altered immune response and gut microbiota have been associated with the pathology of Inflammatory Bowel Diseases (IBD). However, there is limited knowledge of how inflammation is associated with changes in the microbiota. We studied the microbiota in the intestine and faeces as well as the cytokine gene expressions in caecum and colon of a mouse model (Gαi2(-/-)) of colitis, and analysed them in relation to the degrees of inflammation in the colon. The degree of colitis was associated with general changes in the complexity of the microbiota and was corroborated by quantitative analyses of the Bacteroides and Lactobacillus High gene expression levels of IL-17 and IFN-γ in colon and caecum were detected in Gαi2(-/-) mice with moderate and severe colitis. High IL-27 gene expression in the colon of mice with moderate and severe colitis and in the caecum of mice with moderate colitis was also detected. Negative correlations between IL-27 and Bacteroides and Lactobacillus and between IFN-γ and Lactobacillus were detected in caecum. This research indicates that the degree of colitis in IBD correlates with the gene expression of cytokines and with disturbances in the gut microbiota. Furthermore, the caecum could have an important role in the pathology of IBD.

  • 52.
    Rangel, Ignacio
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Ganda-Mall, John-Peter
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Sjöberg, F.
    University of Gothenburg, Gothenburg, Sweden.
    Willen, R.
    Univ Uppsala Hosp, Uppsala, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Alterations in gut microbiota composition in tissues and feces of G alpha i2(-/-) mice with colitis in parallel with enhanced cytokine secretion2013Inngår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 140, s. 168-169Artikkel i tidsskrift (Annet vitenskapelig)
  • 53. Strid, H.
    et al.
    Kumawat, Ashok Kumar
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren Hörnquist, Elisabet
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bohr, J.
    Altered gene expression of IL-6 and rennin in colonic biopsies from collagenous colitis and ulcerative colitis compared to healthy controls2011Inngår i: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 60, nr Suppl. 3, artikkel-id A317Artikkel i tidsskrift (Fagfellevurdert)
  • 54. Strid, Hilja
    et al.
    Kumawat, Ashok
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Tysk, Curt
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren Hörnquist, Elisabet
    Örebro universitet, Institutionen för läkarutbildning.
    Bohr, Johan
    Genuttrycket för Renin och IL-6 i kolonmucosan är förändrad vid kollagen kolit2012Konferansepaper (Annet vitenskapelig)
  • 55.
    Sundin, Johanna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Brummer, Robert
    Örebro universitet, Institutionen för läkarutbildning.
    Hultgren Hörnquist, Elisabet
    Örebro universitet, Institutionen för läkarutbildning.
    Rangel, Ignacio
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Increased number of double positive CD3+ CD8+ CD4+ lamina propria T lymphocyte in gut mucosa of post infectious IBS patients compared to healthy controls2012Inngår i: Neurogastroenterology and Motility, ISSN 1350-1925, E-ISSN 1365-2982, Vol. 24, nr Suppl. 2, s. 104-105Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    Objective: Irritable bowel syndrome (IBS) developed after a gastroenteritis is denoted post infectious IBS (PI-IBS) and is thought to represent a specific patho-physiological entity. Naive CD8+CD45RA+cytotoxic T lymphocytes recognize antigen derived from intracellular bacteria and viruses. Naive CD4+CD45RA+helper T lymphocytes are activated by the antigen from extracellular microorganisms with the help of antigen-presenting cells. Activated CD4+CD45RO+helper T lymphocytes help phagocytes to kill microbes and activate naive B lymphocytes.

    Aim: To characterize subsets of mucosal lymphocytes in PI-IBS with flow cytometry analysis as a lead in identifying new therapeutic methods.

    Methods: 5 PI-IBS patients and 6 healthy individuals were recruited. We performed a distal colonoscopy without bowel cleansing or other preparation. Lamina propria lymphocytes (LPL) and intra-epithelial lymphocytes (IEL) were isolated from sigmodal biopsies and sub classified by CD3, CD4, CD8, CD45RO and CD45RA with flow cytometry.

    Results: We observed a significant deference (P < 0.01, two-tailed Mann–Whitney test) in double positive CD3+CD8+CD4+LPL between PI-IBS patient and healthy controls. We also observed a trend towards increased frequency of CD3+CD4+LPL in PI-IBS patients. Additionally, PI-IBS patients showed an increased frequency of CD3+CD8+LPL and IEL. The proportion of memory / activated CD45RO+CD4+LPL was higher in PI-IBS patients, and its proportion of the CD8+population was lower compared with the healthy controls. On the contrary the proportion of naive CD45RA+CD4+LPL was lower in PI-IBS patients compared with healthy controls. However, we found no differences in the distribution naı¨ve / activated CD4+ and CD8+IEL between PI-IBS patients and healthy controls.

    Conclusion: The difference in double positive (DP) CD3+CD8+CD4+LPL seen between PI-IBS patient and healthy controls is consistent with findings of increased number DP T cells in target organs in immuno-inflammatory conditions. Our analysis revealed that the normal gut has a greater variation in the prevalence of CD3+CD4+and CD4+CD45RO+LPL than that of PI-IBS patients. These findings confirm aberrant mucosal subsets of lymphocytes. However, more subjects need to be included in the study in order to draw firm conclusions.

  • 56. Sundin, Johanna
    et al.
    Kumawat, Ashok Kumar
    Rangel, I.
    Brummer, Robert
    Hultgren Hörnquist, Elisabet
    Örebro universitet, Institutionen för läkarutbildning.
    Karakterisering av T-lymfocyter från tarmmukosan hos patienter med postinfektiös IBS2012Konferansepaper (Annet vitenskapelig)
  • 57.
    Sundin, Johanna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Rangel, Ignacio
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Fuentes, S
    Department of Microbiology, Wageningen University, Wageningen,The Netherlands.
    Jong, Heikamp-de
    Department of Microbiology, Wageningen University, Wageningen,The Netherlands.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    de Vos, W M
    Department of Microbiology, Wageningen University, Wageningen,The Netherlands; Departments of Bacterology & Immunology and Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
    Brummer, Robert-Jan
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Altered faecal and mucosal microbial composition in post-infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress2015Inngår i: Alimentary Pharmacology and Therapeutics, ISSN 0269-2813, E-ISSN 1365-2036, Vol. 41, nr 4, s. 342-351Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: A subset of irritable bowel syndrome (IBS) patients, denoted post-infectious IBS (PI-IBS), develop symptoms after an enteric infection. Bacterial dysbiosis and mucosal inflammation have been proposed to be involved in the pathophysiology of this entity.

    Aim: To characterise the mucosal and faecal microbiota in PI-IBS, general IBS and healthy controls, and to investigate associations between the microbiota and the mucosal immune system.

    Methods: Mucosal biopsies and faeces were collected from 13 PI-IBS patients, 19 general IBS patients and 16 healthy controls. Global bacterial composition was determined by generating 16S rRNA amplicons that were examined by phylogenetic microarray hybridisation, principal component and redundancy analysis. We correlated previously reported lymphocyte proportions with the microbiota.

    Results: Faecal microbiota composition of PI-IBS patients differed significantly from both general IBS patients and healthy controls (P < 0.02). Both mucosal (P < 0.01) and faecal (P = 0.05) microbial diversity were reduced in PI-IBS compared to healthy controls. In the intraepithelial lymphocytes the previously published proportion of CD8+ CD45RA+ was negatively correlated with mucosal microbial diversity (P < 0.005). The previously published number of lamina propria lymphocytes was negatively correlated with mucosal microbial diversity (P < 0.05). Faecal microbial diversity was significantly negatively correlated with the Hospital Anxiety and Depression scale (P < 0.05).

    Conclusions: We present data that distinguishes the intestinal microbiota of PI-IBS patients from that of both general IBS patients and HC. The microbial composition is significantly associated with the HADs score and alterations in lymphocyte subsets proportions.

  • 58.
    Sundin, Johanna
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Rangel, Ignacio
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro universitet, Institutionen för läkarutbildning.
    Kumawat, Ashok K
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    Brummer, Robert J
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Aberrant mucosal lymphocyte number and subsets in the colon of post-infectious irritable bowel syndrome patients2014Inngår i: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 49, nr 9, s. 1068-1075Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Irritable bowel syndrome (IBS) is characterized by chronic abdominal symptoms such as pain, discomfort, and altered bowel habits. A subset of IBS patients, denoted as post-infectious IBS (PI-IBS) patients, develop symptoms after an enteric infection. Distinct abnormalities in the gut mucosa, including mucosal inflammation, have been proposed to contribute to or be the cause of PI-IBS. This study investigated lymphocyte subsets in PI-IBS patients compared to healthy controls.

    Materials and methods: Ten PI-IBS patients and nine healthy controls participated. All PI-IBS patients met the Rome III diagnostic criteria for IBS and reported sustained symptoms at least 1 year after an episode of acute gastroenteritis. Intraepithelial lymphocytes and lamina propria lymphocytes (LPLs), isolated from mucosal tissue samples, were stained and analyzed for a comprehensive set of cell markers using flow cytometry.

    Results: The number of LPLs in PI-IBS was significantly increased compared to those in healthy controls (p < 0.05). PI-IBS patients showed significantly increased proportions of CD45RO(+) CD4(+) activated/memory T cells (p < 0.05) and double-positive CD4(+) CD8(+) cells (p < 0.05), respectively, in the lamina propria. The number of CD19(+) LPLs was decreased in PI-IBS patients compared to healthy controls (p < 0.001).

    Conclusion: This study presents new evidence that PI-IBS is associated with a sustained aberrant mucosal immune response and support future studies of anti-inflammatory or immune-modulating treatments in these patients.

12 51 - 58 of 58
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