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  • 51.
    Burman, Joachim
    et al.
    Department of Neuroscience, Uppsala University, Uppsala, Sweden; Department of Neurology, Uppsala University Hospital, Uppsala, Sweden .
    Iacobaeus, Ellen
    Neuroimmunology Unit, Department of Clinical Neuroscience, Karolinska Institute Solna, Center for Molecular Medicine, Stockholm, Sweden.
    Svenningsson, Anders
    Department of Pharmacology and Clinical Neuroscience, Umeå University and University Hospital of Northern Sweden, Umeå, Sweden .
    Lycke, Jan
    Department of Neurology, Institute of Neuroscience and Physiology, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Gunnarsson, Martin
    Örebro universitet, Institutionen för läkarutbildning. Department of Neurology, Örebro University Hospital, Örebro, Sweden .
    Nilsson, Petra
    Department of Neurology, Skåne University Hospital Lund, Lund, Sweden.
    Vrethem, Magnus
    Neurology and Clinical Neurophysiology, Faculty of Health Sciences, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Neurology and Neurophysiology, County Council of Östergötland, Linköping, Sweden .
    Fredrikson, Sten
    Department of Clinical Neuroscience, Karolinska Institute Huddinge, Stockholm, Sweden .
    Martin, Claes
    Neurology Unit, Division of Internal Medicine, Danderyd Hospital, Karolinska Institute, Stockholm, Sweden .
    Sandstedt, Anna
    Department of Hematology, Linköping University Hospital, Linköping, Sweden .
    Uggla, Bertil
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Division of Hematology, Department of Medicine, Örebro University Hospital, Örebro, Sweden.
    Lenhoff, Stig
    Department of Hematology and Coagulation, Skåne University Hospital, Lund, Sweden .
    Johansson, Jan-Erik
    Department of Hematology and Coagulation, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Isaksson, Cecilia
    Department of Radiosciences, Umeå University, Umeå, Sweden .
    Hägglund, Hans
    Division of Hematology, Department of Medical Science, Uppsala University Hospital, Uppsala, Sweden.
    Carlson, Kristina
    Division of Hematology, Department of Medical Science, Uppsala University Hospital, Uppsala, Sweden.
    Fagius, Jan
    Department of Neuroscience, Uppsala University, Uppsala, Sweden; Department of Neurology, Uppsala University Hospital, Uppsala, Sweden .
    Autologous haematopoietic stem cell transplantation for aggressive multiple sclerosis: the Swedish experience2014Ingår i: Journal of Neurology, Neurosurgery and Psychiatry, ISSN 0022-3050, E-ISSN 1468-330X, Vol. 85, nr 10, s. 1116-1121Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Autologous haematopoietic stem cell transplantation (HSCT) is a viable option for treatment of aggressive multiple sclerosis (MS). No randomised controlled trial has been performed, and thus, experiences from systematic and sustained follow-up of treated patients constitute important information about safety and efficacy. In this observational study, we describe the characteristics and outcome of the Swedish patients treated with HSCT for MS.

    Methods: Neurologists from the major hospitals in Sweden filled out a follow-up form with prospectively collected data. Fifty-two patients were identified in total; 48 were included in the study and evaluated for safety and side effects; 41 patients had at least 1 year of follow-up and were further analysed for clinical and radiological outcome. In this cohort, 34 patients (83%) had relapsing-remitting MS, and mean follow-up time was 47 months.

    Results: At 5 years, relapse-free survival was 87%; MRI event-free survival 85%; expanded disability status scale (EDSS) score progression-free survival 77%; and disease-free survival (no relapses, no new MRI lesions and no EDSS progression) 68%. Presence of gadolinium-enhancing lesions prior to HSCT was associated with a favourable outcome (disease-free survival 79% vs 46%, p=0.028). There was no mortality. The most common long-term side effects were herpes zoster reactivation (15%) and thyroid disease (8.4%).

    Conclusions: HSCT is a very effective treatment of inflammatory active MS and can be performed with a high degree of safety at experienced centres.

  • 52.
    Byström, David
    Örebro universitet, Institutionen för läkarutbildning.
    En jämförelse av patientupplevelsen vidpatientbokade respektive kallade gastroskopier– en enkätstudie.2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 53.
    Bäckman, Anders
    et al.
    Örebro universitet, Institutionen för medicinska vetenskaper. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden.
    Makdoumi, Karim
    Örebro universitet, Institutionen för medicinska vetenskaper. Department of Ophthalmology, Örebro University Hospital, Örebro, Sweden; Centre for Health Care Sciences, Örebro University Hospital, Örebro, Sweden.
    Mortensen, Jes
    Department of Ophthalmology, Ryhov County Hospital, Jönköping, Sweden.
    Crafoord, Sven
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Ophthalmology, Örebro University Hospital, Örebro, Sweden.
    The efficiency of cross-linking methods in eradication of bacteria is influenced by the riboflavin concentration and the irradiation time of ultraviolet light2014Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, nr 7, s. 656-661Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To determine bacterial eradication using numerous riboflavin concentrations and different ultraviolet light A (UVA) radiant and exposure time in an experimental model.

    Methods: Dilutions of Staphylococcus epidermidis were mixed with riboflavin at varying concentrations (0.007-0.09%). Effects on bacterial growth were evaluated after 0, 3, 6, 30 and 60min of UVA exposure (irradiance 30 and 3mW/cm(2)). Standard settings of UVA were compared with high-power UVA approach. Different fluid thicknesses of the exposed dilutions were also examined to improve the model.

    Results: Bacterial eradication (%) was increased after 60 compared with 30min of UVA exposure for concentrations of 0.03-0.07% but not for 0.09% riboflavin. There was a significant difference between the efficacy between 0.03 and 0.09% and eradication dropped from 80% to 50% (p=0.01). A correlation could be calculated for the amount of riboflavin at 60min of UVA and the ability to kill bacteria (p=0.01). The antibacterial effect was more pronounced when the tested bacterial suspension thickness was reduced. High-power UVA method was less potent in microbial elimination, eradicating only 60% of bacteria after 6min versus 97-99% after 60min in the low-power setting, compared with respective controls (p=0.02).

    Conclusions: In these in vitro experiments, a longer UVA exposure time in combination with lower riboflavin levels were found to be favourable in killing bacteria as compared to the standard cross-linking settings. Further studies are needed to evaluate the clinical relevance of these findings.

  • 54.
    Cajander, Sara
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden.
    Bäckman, Anders
    Örebro universitet, Institutionen för medicinska vetenskaper. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden.
    Tina, Elisabet
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden.
    Strålin, Kristoffer
    Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden; Department of Infectious Diseases, Karolinska University Hospital, Huddinge, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Källman, Jan
    Örebro universitet, Institutionen för medicinska vetenskaper. Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden.
    Preliminary results in quantitation of HLA-DRA by real-time PCR: a promising approach to identify immunosuppression in sepsis2013Ingår i: Critical Care, ISSN 1364-8535, E-ISSN 1466-609X, Vol. 17, nr 5, artikel-id R223Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Introduction: Reduced monocyte human leukocyte antigen (mHLA)-DR surface expression in the late phase of sepsis is postulated as a general biomarker of sepsis-induced immunosuppression and an independent predictor of nosocomial infections. However, traditional monitoring of mHLA-DR by flow cytometry has disadvantages due to specific laboratory requirements. An mRNA-based HLA-DR monitoring by polymerase chain reaction (PCR) would improve the clinical usage and facilitate conduction of large multicenter studies. In this study, we evaluated an mRNA-based HLA-DR monitoring by quantitative real-time PCR (qRT-PCR) as an alternative method to traditional flow cytometry.

    Methods: Fifty-nine patients with sepsis and blood culture growing pathogenic bacteria were studied. Blood samples were collected at day 1 or 2 after admission, for measurement of mHLA-DR by flow cytometry and mRNA expression of HLA-DRA and class II transactivator (CIITA) by qRT-PCR. Blood samples from blood donors were used as controls (n = 30).

    Results: A significant reduced expression of mHLA-DR, HLA-DRA, and CIITA was seen in septic patients compared with controls. HLA-DRA mRNA level in whole blood was highly correlated with surface expression of mHLA-DR.

    Conclusions: Patients with sepsis display a diminished expression of HLA-DR at the monocyte surface as well as in the gene expression at the mRNA level. The mRNA expression level of HLA-DRA monitored by qRT-PCR correlates highly with surface expression of HLA-DR and appears to be a possible future biomarker for evaluation of immunosuppression in sepsis.

  • 55.
    Callerud, Sofia
    Örebro universitet, Institutionen för läkarutbildning.
    Is there a cure for HIV?: - A systematic literature review2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 56.
    Carlsson, Jessica
    et al.
    Örebro universitet, Hälsoakademin.
    Davidsson, Sabina
    Örebro universitet, Hälsoakademin.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Örebro, Sweden.
    Karlsson, Mats
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Örebro, Sweden.
    Lubovac, Zelmina
    University of Skövde, Skövde, Sweden.
    Andrén, Ove
    Örebro universitet, Institutionen för läkarutbildning. Örebro University Hospital, Örebro, Sweden.
    Olsson, Björn
    University of Skövde, Skövde, Sweden.
    Klinga-Levan, Karin
    University of Skövde, Skövde, Sweden.
    A miRNA expression signature that separates between normal and malignant prostate tissues2011Ingår i: Cancer Cell International, ISSN 1475-2867, E-ISSN 1475-2867, nr 11, s. 14-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background

    MicroRNAs (miRNAs) constitute a class of small non-coding RNAs that post-transcriptionally regulate genes involved in several key biological processes and thus are involved in various diseases, including cancer. In this study we aimed to identify a miRNA expression signature that could be used to separate between normal and malignant prostate tissues.

    Results

    Nine miRNAs were found to be differentially expressed (p <0.00001). With the exception of two samples, this expression signature could be used to separate between the normal and malignant tissues. A cross-validation procedure confirmed the generality of this expression signature. We also identified 16 miRNAs that possibly could be used as a complement to current methods for grading of prostate tumor tissues.

    Conclusions

    We found an expression signature based on nine differentially expressed miRNAs that with high accuracy (85%) could classify the normal and malignant prostate tissues in patients from the Swedish Watchful Waiting cohort. The results show that there are significant differences in miRNA expression between normal and malignant prostate tissue, indicating that these small RNA molecules might be important in the biogenesis of prostate cancer and potentially useful for clinical diagnosis of the disease.

  • 57.
    Carlsson, Jessica
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län.
    Helenius, Gisela
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Karlsson, Mats G.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Andrén, Ove
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Örebro University Hospital, Region Örebro County, Örebro, Sweden.
    Klinga-Levan, Karin
    Systems Biology Research Centre, Tumor Biology, School of Life Sciences, University of Skövde, Skövde, Sweden.
    Olsson, Björn
    Systems Biology Research Centre, Bioinformatics, School of Life Sciences, University of Skövde, Skövde, Sweden.
    Differences in microRNA expression during tumor development in the transition and peripheral zones of the prostate2013Ingår i: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 13, artikel-id 362Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: The prostate is divided into three glandular zones, the peripheral zone (PZ), the transition zone (TZ), and the central zone. Most prostate tumors arise in the peripheral zone (70-75%) and in the transition zone (20-25%) while only 10% arise in the central zone. The aim of this study was to investigate if differences in miRNA expression could be a possible explanation for the difference in propensity of tumors in the zones of the prostate.

    Methods: Patients with prostate cancer were included in the study if they had a tumor with Gleason grade 3 in the PZ, the TZ, or both (n=16). Normal prostate tissue was collected from men undergoing cystoprostatectomy (n=20). The expression of 667 unique miRNAs was investigated using TaqMan low density arrays for miRNAs. Student's t-test was used in order to identify differentially expressed miRNAs, followed by hierarchical clustering and principal component analysis (PCA) to study the separation of the tissues. The ADtree algorithm was used to identify markers for classification of tissues and a cross-validation procedure was used to test the generality of the identified miRNA-based classifiers.

    Results: The t-tests revealed that the major differences in miRNA expression are found between normal and malignant tissues. Hierarchical clustering and PCA based on differentially expressed miRNAs between normal and malignant tissues showed perfect separation between samples, while the corresponding analyses based on differentially expressed miRNAs between the two zones showed several misplaced samples. A classification and cross-validation procedure confirmed these results and several potential miRNA markers were identified.

    Conclusions: The results of this study indicate that the major differences in the transcription program are those arising during tumor development, rather than during normal tissue development. In addition, tumors arising in the TZ have more unique differentially expressed miRNAs compared to the PZ. The results also indicate that separate miRNA expression signatures for diagnosis might be needed for tumors arising in the different zones. MicroRNA signatures that are specific for PZ and TZ tumors could also lead to more accurate prognoses, since tumors arising in the PZ tend to be more aggressive than tumors arising in the TZ.

  • 58.
    Cerantola, Yannick
    et al.
    University Hospital of Lausanne, Lausanne, Switzerland.
    Valerio, Massimo
    University Hospital of Lausanne, Lausanne, Switzerland.
    Persson, Beata
    University Hospital of Örebro, Örebro, Sweden.
    Jichlinski, Patrice
    University Hospital of Lausanne, Lausanne, Switzerland.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    Hubner, Martin
    University Hospital of Lausanne, Lausanne, Switzerland.
    Kassouf, Wassim
    McGill University, Montreal, Canada.
    Muller, Stig
    Akershus University Hospital, Oslo, Norway.
    Baldini, Gabriele
    McGill University, Montreal, Canada.
    Carli, Francesco
    McGill University, Montreal, Canada.
    Naesheimh, Torvind
    University Hospital of Northern Norway, Tromsø, Norway.
    Ytrebo, Lars
    University Hospital of Northern Norway, Tromsø, Norway.
    Revhaug, Arthur
    University Hospital of Northern Norway, Tromsø, Norway.
    Lassen, Kristoffer
    University Hospital of Northern Norway, Tromsø, Norway.
    Knutsen, Tore
    University Hospital of Northern Norway, Tromsø, Norway.
    Aarsether, Erling
    University Hospital of Northern Norway, Tromsø, Norway.
    Wiklund, Peter
    Karolinska University Hospital, Stockholm, Sweden.
    Patel, Hitendra R H
    University Hospital of Northern Norway, Tromsø, Norway.
    Guidelines for perioperative care after radical cystectomy for bladder cancer: enhanced Recovery After Surgery (ERAS(®)) society recommendations2013Ingår i: Clinical Nutrition, ISSN 0261-5614, E-ISSN 1532-1983, Vol. 32, nr 6, s. 879-887Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE: Enhanced recovery after surgery (ERAS) pathways have significantly reduced complications and length of hospital stay after colorectal procedures. This multimodal concept could probably be partially applied to major urological surgery.

    OBJECTIVES: The primary objective was to systematically assess the evidence of ERAS single items and protocols applied to cystectomy patients. The secondary objective was to address a grade of recommendation to each item, based on the evidence and, if lacking, on consensus opinion from our ERAS Society working group.

    EVIDENCE ACQUISITION: A systematic literature review was performed on ERAS for cystectomy by searching EMBASE and Medline. Relevant articles were selected and quality-assessed by two independent reviewers using the GRADE approach. If no study specific to cystectomy was available for any of the 22 given items, the authors evaluated whether colorectal guidelines could be extrapolated.

    EVIDENCE SYNTHESIS: Overall, 804 articles were retrieved from electronic databases. Fifteen articles were included in the present systematic review and 7 of 22 ERAS items were studied. Bowel preparation did not improve outcomes. Early nasogastric tube removal reduced morbidity, bowel recovery time and length of hospital stay. Doppler-guided fluid administration allowed for reduced morbidity. A quicker bowel recovery was observed with a multimodal prevention of ileus, including gum chewing, prevention of PONV and minimally invasive surgery.

    CONCLUSIONS: ERAS has not yet been widely implemented in urology and evidence for individual interventions is limited or unavailable. The experience in other surgical disciplines encourages the development of an ERAS protocol for cystectomy.

  • 59.
    Christiansen, Jesper
    Örebro universitet, Institutionen för läkarutbildning.
    Evaluation of the microRNA 21/10b ratio as a prognostic tool in clear cell renal cell carcinoma2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 60.
    Currie, Andrew
    et al.
    Department of Surgery, St Mark's Hospital, Academic Institute, Imperial College London, London, United Kingdom.
    Burch, Jennifer
    Department of Surgery, St Mark's Hospital, Academic Institute, Imperial College London, London, United Kingdom.
    Jenkins, John T.
    Department of Surgery, St Mark's Hospital, Academic Institute, Imperial College London, London, United Kingdom.
    Faiz, Omar
    Department of Surgery, St Mark's Hospital, Academic Institute, Imperial College London, London, United Kingdom.
    Kennedy, Robin H.
    Department of Surgery, St Mark's Hospital, Academic Institute, Imperial College London, London, United Kingdom.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning. Department of Surgery, Örebro University Hospital, Örebro, Sweden.
    Demartines, Nicolas
    CHUV Lausanne Surg, Lausanne, Switzerland.
    Hjern, Fredrik
    Danderyds Sjukhus, Danderyd, Sweden.
    Norderval, Stig
    University Hospital of North Norway, Tromsö, Norway.
    Lassen, Kristoffer
    University Hospital of North Norway, Tromsö, Norway.
    Revhaug, Andarthur
    University Hospital of North Norway, Tromsö, Norway.
    Koczkas, Tomas
    Östersunds Sjukhus, Östersund, Sweden.
    Nygren, Jonas
    Ersta Sjukhus, Stockholm, Sweden.
    Gustafsson, Ulf
    Ersta Sjukhus, Stockholm, Sweden.
    Kornfeld, Dan
    Kirurgkliniken St Göran, Stockholm, Sweden.
    Slim, Karem
    University Hospital, Clermont Ferrand, France.
    Hill, Andrew
    Middlemore Hospital, Auckland, New Zealand.
    Soop, Mattias
    North Shore Hospital, Auckland, New Zealand.
    Carlander, Johan
    Västerås Centrallasarett, Västerås, Sweden.
    Lundberg, Owe
    Kirurgkliniken Umeå, Umeå, Sweden.
    Fearon, Ken
    Western General Hospital, Edinburgh, United Kingdom.
    Kennedy, Robin
    St Marks Hospital, London, England..
    The Impact of Enhanced Recovery Protocol Compliance on Elective Colorectal Cancer Resection Results From an International Registry2015Ingår i: Annals of Surgery, ISSN 0003-4932, E-ISSN 1528-1140, Vol. 261, nr 6, s. 1153-1159Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: The ERAS (enhanced recovery after surgery) care has been shown in randomized clinical trials to improve outcome after colorectal surgery compared to traditional care. The impact of different levels of compliance and specific elements, particularly out with a trial setting, is poorly understood.

    Objective: This study evaluated the individual impact of specific patient factors and perioperative enhanced recovery protocol compliance on postoperative outcome after elective primary colorectal cancer resection.

    Methods: The international, multicenter ERAS registry data, collected between November 2008 and March 2013, was reviewed. Patient demographics, disease characteristics, and perioperative ERAS protocol compliance were assessed. Linear regression was undertaken for primary admission duration and logistic regression for the development of any postoperative complication.

    Findings: A total of 1509 colonic and 843 rectal resections were undertaken in 13 centers from 6 countries. Median length of stay for colorectal resections was 6 days, with readmissions in 216 (9.2%), complications in 948 (40%), and reoperation in 167 (7.1%) of 2352 patients. Laparoscopic surgery was associated with reduced complications [odds ratio (OR) = 0.68; P < 0.001] and length of stay (OR = 0.83, P < 0.001). Increasing ERAS compliance was correlated with fewer complications (OR = 0.69, P < 0.001) and shorter primary hospital admission (OR = 0.88, P < 0.001). Shorter hospital stay was associated with preoperative carbohydrate and fluid loading (OR = 0.89, P = 0.001), and totally intravenous anesthesia (OR= 0.86, P < 0.001); longer stay was associated with intraoperative epidural analgesia (OR = 1.07, P = 0.019). Reduced postoperative complications were associated with restrictive perioperative intravenous fluids (OR = 0.35, P < 0.001).

    Conclusions: This analysis has demonstrated that in a large, international cohort of patients, increasing compliance with an ERAS program and the use of laparoscopic surgery independently improve outcome.

  • 61.
    Da Silva, Fatima
    Örebro universitet, Institutionen för läkarutbildning.
    Presence of postoperative complication in smoking compared to non-smoking patients who underwent bariatric surgery in Örebro County: a quality register study.2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 62.
    Dahlberg, Anton
    Örebro universitet, Institutionen för läkarutbildning.
    Photographic documentation of patch tests2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 63.
    Dahlbom, Moa
    Örebro universitet, Institutionen för läkarutbildning.
    Gene Expression of CTGF in Human Dermal Fibroblasts When Exposed to TGF-β and IL-1α2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 64.
    Dahlgren, Hedda
    Örebro universitet, Institutionen för läkarutbildning.
    Är akutsjukvården jämställd?: Genusperspektiv på akutmottagningens verksamhet vid Universitetssjukhuset Örebro2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 65.
    Damberg, Jonas
    Örebro universitet, Institutionen för läkarutbildning.
    Manganese speciation in blood of welders, ultrafiltration as an alternative method to size-exclusion chromatography2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 66.
    Davidsson, Sabina
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Mölling, Paula
    Unemo, Magnus
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Rider, Jennifer R.
    Karlsson, Mats G.
    Andersson, Swen-Olof
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Elgh, Fredrik
    Andrén, Ove
    Örebro universitet, Institutionen för läkarutbildning.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning.
    Prevalence and typing of Propionibacterium acnes in prostate tissue obtained from men with prostate cancer and from health controlsManuskript (preprint) (Övrigt vetenskapligt)
  • 67.
    Davidsson, Sabina
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Urology, Örebro University Hospital, Örebro, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Elgh, Fredrik
    Örebro universitet, Hälsoakademin. Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
    Olsson, Jan
    Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
    Andrén, Ove
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Urology, Örebro University Hospital, Örebro, Sweden.
    Unemo, Magnus
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Mölling, Paula
    Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Multilocus sequence typing and repetitive-sequence-based PCR (DiversiLab) for molecular epidemiological characterization of Propionibacterium acnes isolates of heterogeneous origin2012Ingår i: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 18, nr 4, s. 392-399Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Propionibacterium acnes is a gram-positive bacillus predominantly found on the skin. Although it is considered an opportunistic pathogen it is also been associated with severe infections. Some specific P. acnes subtypes are hypothesized to be more prone to cause infection than others. Thus, the aim of the present study was to investigate the ability to discriminate between P. acnes isolates of a refined multilocus sequence typing (MLST) method and a genotyping method, DiversiLab, based on repetitive-sequence-PCR technology.

    The MLST and DiversiLab analysis were performed on 29 P. acnes isolates of diverse origins; orthopedic implant infections, deep infections following cardiothoracic surgery, skin, and isolates from perioperative tissue samples from prostate cancer. Subtyping was based on recA, tly, and Tc12S sequences.

    The MLST analysis identified 23 sequence types and displayed a superior ability to discriminate P. acnes isolates compared to DiversiLab and the subtyping. The highest discriminatory index was found when using seven genes. DiversiLab was better able to differentiate the isolates compared to the MLST clonal complexes of sequence types.

    Our results suggest that DiversiLab can be useful as a rapid typing tool for initial discrimination of P. acnes isolates. When better discrimination is required, such as for investigations of the heterogeneity of P. acnes isolates and its involvement in different pathogenic processes, the present MLST protocol is valuable.

  • 68.
    Davidsson, Åke
    Örebro universitet, Institutionen för läkarutbildning.
    Allergidagen med temat: "Födoämnesöverkänslighet"2014Ingår i: Lung och alllergiforum, ISSN 2000-5237, nr 4, s. 29-33Artikel i tidskrift (Övrig (populärvetenskap, debatt, mm))
  • 69.
    Davidsson, Åke
    et al.
    Örebro universitet, Institutionen för läkarutbildning.
    Holmström, Mats
    Näspolyper och den täppta näsan2014Ingår i: Fagbladet Allergi i praksis, ISSN 0806-5462, nr 2, s. 16-20Artikel i tidskrift (Refereegranskat)
  • 70.
    De Brun Mans, Sebastian
    Örebro universitet, Institutionen för läkarutbildning.
    Effect on nasal airway patency by closed reduction treatment in patients with nasal fractures.2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 71.
    Demirel, Isak
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kinnunen, Annica
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Önnberg, Anna
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
    Persson, Katarina
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Comparison of host response mechanisms evoked by extended spectrum beta lactamase (ESBL)- and non-ESBL-producing uropathogenic E. coli2013Ingår i: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 13, artikel-id 181Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Infections caused by extended spectrum beta-lactamases (ESBL)-producing bacteria have been emerging worldwide and the majority of ESBL-producing E. coli strains are isolated from patients with urinary tracts infections. The purpose of this study was to compare the host-response mechanisms in human polymorphonucleated leukocytes (PMN) and renal epithelial cells when stimulated by ESBL-or non-ESBL-producing uropathogenic E. coli (UPEC) isolates. The host-pathogen interaction of these ESBL-producing strains in the urinary tract is not well studied.

    Results: The ability of ESBL strains to evoke ROS-production from PMN cells was significantly higher than that of the non-ESBL strains. The growth of ESBL strains was slightly suppressed in the presence of PMN compared to non-ESBL strains after 30 min and 2 h, but the opposite was observed after 5 and 6 h. The number of migrating PMN was significantly higher in response to ESBL strains compared to non-ESBL strains. Stimulation of A498 cells with ESBL strains elicited lower production of IL-6 and IL-8 compared to non-ESBL strains.

    Conclusion: Significant differences in host-response mechanisms were identified when host cells were stimulated by ESBL-or non-ESBL producing strains. The obtained results on the early interactions of ESBL-producing strains with the host immune system may provide valuable information for management of these infections.

  • 72.
    Demirel, Isak
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kruse, Robert
    Örebro universitet, Institutionen för läkarutbildning.
    Önnberg, Anna
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Persson, Katarina
    Örebro universitet, Institutionen för läkarutbildning.
    Ceftibuten-induced filamentation of extended spectrum beta lactamase (ESBL)-producing uropathogenic Escherichia coli alters host cell responses during an in vitro infection2015Ingår i: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 78, s. 52-62Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Inadequate and delayed antibiotic treatment of extended spectrum beta-lactamase (ESBL)-producing isolates have been associated with increased mortality of affected patients. The purpose of this study was to compare the host response of human renal epithelial cells and polymorphonuclear leucocyte (PMN) cells when infected by ESBL-producing uropathogenic Escherichia coli (UPEC) isolates in the presence or absence of ineffective antibiotics.

    The renal epithelial cell line A498 and PMN cells were stimulated with ESBL-producing UPEC isolates in the presence or absence of three different antibiotics (trimetoprim, ceftibuten and ciprofloxacin). Host cell responses were evaluated as release of cytokines (IL-6, IL-8), reactive oxygen species (ROS), ATP and endotoxins. Bacterial morphology and PMN phagocytosis were evaluated by microscopy.

    In the presence of ceftibuten, 2 out of 3 examined ESBL-isolates changed their morphology into a filamentous form. The presence of ceftibuten enhanced IL-6, IL-8 and ROS-production from host cells, but only from cells stimulated by the filamentous isolates. The bacterial supernatant and not the filamentous bacteria per se was responsible for the increased release of IL-6, IL-8 and ROS. Increased endotoxin and ATP levels were found in the bacterial supernatants from filamentous isolates. Apyrase decreased IL-6 secretion from A498 cells and polymyxin B abolished the increased ROS-production from PMN cells. PMN were able to inhibit the bacterial growth of some ESBL-isolates in the presence of ceftibuten.

    In conclusion, antibiotic-induced filamentation of ESBL-producing UPEC isolates and the associated release of ATP and endotoxins can alter the host cell response in the urinary tract.

  • 73.
    Dimic, Anna
    Örebro universitet, Institutionen för läkarutbildning.
    Theory of Mind and cognition in individuals with hearing impairment: - A Pilot Study2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 74.
    Ding, Zhen-Yu
    et al.
    Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Country Council of Östergötland, University of Linköping, Linköping, Sweden; Department of Thoracic Oncology, Cancer Center, and the State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
    Zhang, Hong
    Örebro universitet, Institutionen för läkarutbildning.
    Adell, Gunnar
    Department of Oncology, Karolinska University Hospital, Stockholm, Sweden.
    Olsson, Birgit
    Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Country Council of Östergötland, University of Linköping, Linköping, Sweden.
    Sun, Xiao-Feng
    Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Country Council of Östergötland, University of Linköping, Linköping, Sweden.
    Livin expression is an independent factor in rectal cancer patients with or without preoperative radiotherapy2013Ingår i: Radiation Oncology, ISSN 1748-717X, E-ISSN 1748-717X, Vol. 8, nr 1, artikel-id 281Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: This study was aimed to investigate the expression significance of Livin in relation to radiotherapy (RT), clinicopathological and biological factors of rectal cancer patients.

    Methods: This study included 144 primary rectal cancer patients who participated in a Swedish clinical trial of preoperative radiotherapy. Tissue microarray samples from the excised primary rectal cancers, normal mucosa and lymph node metastases were immunostained with Livin antibody. The proliferation of colon cancer cell lines SW620 and RKO was assayed after Livin knock-down.

    Results: The expression of Livin was significantly increased from adjacent (P = 0.051) or distant (P = 0.028) normal mucosa to primary tumors. 15.4% (2/13) and 39.7% (52/131) patients with Livin-negative and positive tumors died at 180 months after surgery, and the difference tended to be statistically significant (P = 0.091). In multivariate analyses, the difference achieved statistical significance, independent of TNM stage, local and distant recurrence, grade of differentiation, gender, and age (odds ratio = 5.09, 95% CI: 1.01-25.64, P = 0.048). The in vitro study indicated colon cancer cells with Livin knock-down exhibited decreased proliferation compared with controls after RT.

    Conclusions: The expression of Livin was was independently related to survival in rectal cancer patients, suggesting Livin as a useful prognostic factor for rectal cancer patients.

  • 75.
    Edbom, Katarina
    Örebro universitet, Institutionen för läkarutbildning.
    Optimizing medium for culturing adipose-derived mesenchymal stem cells (ADSC)2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 76.
    Ehlersson, Gustaf
    Örebro universitet, Institutionen för läkarutbildning.
    Phenotypic characterization of coagulase-negative staphylococci isolated from bacteremia in the newborn, with special focus on S. capitis 2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 77.
    Ekbäck, Maria Palmetun
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Region Örebro län. Department of Dermatology, Örebro University Hospital, Örebro, Sweden; Department of Pharmacology and Therapeutics, Örebro County Council, Örebro, Sweden.
    Lindberg, Magnus
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Dermatology, University Hospital Örebro, Örebro, Sweden.
    Benzein, Eva
    Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Department of Health and Caring Science, Linnaeus University, Kalmar, Sweden.
    Årestedt, Kristofer
    Department of Health and Caring Science, Linnaeus University, Kalmar, Sweden; Department of Medical and Health Sciences, Linköping University, Linköping, Sweden; Palliative Research Centre, Ersta Sköndal University College and Hospital, Stockholm, Sweden .
    Social support: an important factor for quality of life in women with hirsutism2014Ingår i: Health and Quality of Life Outcomes, ISSN 1477-7525, E-ISSN 1477-7525, Vol. 12, artikel-id 183Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Women with hirsutism have reported imparied health and health-related quality of life (HRQoL). Social support is a factor that might increase HRQoL in chronic diseases, but little is known about this association among women with hirsutism.

    Aim: The aim of the study was to describe social support and explore its association with HRQoL among women with hirsutism. A further aim was to compare HRQoL in women with hirsutism with a Swedish normal population.

    Methods: A questionnaire including socio-demographic questions, Short-Form Health Survey (SF-36), the Multidimensional Scale of Perceived Social Support (MSPSS), and a self-estimation of hairiness using the Ferriman-Gallway scale (F-G scale) was answered by 127 women with hirsutism.

    Results: Multiple regression analyses showed significant associations between social support and all health dimensions in the SF-36, also after the model was adjusted for age, hairiness and body mass index. Compared to the normal Swedish population, women with hirsutism reported significantly lower HRQoL in all dimensions of the SF-36 (p < 0.01).

    Conclusions: There is a significant positive association between social support and HRQoL, demonstrating its importance for the ability to adapt to problems associated with hirsutism. As women with hirsutism reported poorer HRQoL compared to the normal population, social support may be a factor to consider in clinical practice.

  • 78.
    Ekelund, Ulf
    et al.
    Medical Research Council Epidemiology Unit, Cambridge, UK; Department of Physical Education and Health, Örebro University, Örebro, Sweden.
    Särnblad, Stefan
    Örebro universitet, Institutionen för läkarutbildning. Department of Paediatrics, Örebro University Hospital, Örebro, Sweden.
    Brage, Sören
    Medical Research Council Epidemiology Unit, Cambridge, UK.
    Ryberg, John
    Department of Paediatrics, Örebro University Hospital, Örebro, Sweden.
    Wareham, Nicholas J.
    Medical Research Council Epidemiology Unit, Cambridge, UK.
    Åman, Jan
    Department of Paediatrics, Örebro University Hospital, Örebro, Sweden.
    Does physical activity equally predict gain in fat mass among obese and nonobese young adults?2007Ingår i: International Journal of Obesity, ISSN 0307-0565, E-ISSN 1476-5497, Vol. 31, nr 1, s. 65-71Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Differences in energy metabolism and physical activity (PA) may contribute to the long-term regulation of body weight (BW).

    OBJECTIVE: To examine the associations between metabolic determinants, energy expenditure and objectively measured components of PA with change in BW and fat mass (FM). DESIGN: Prospective (4 years.), case-control study in obese (n=13) and normal weight (n=15) young adults.

    MEASUREMENTS: At baseline, we measured resting metabolic rate, substrate oxidation, movement economy (ml O(2) kg(-1) min(-1)), aerobic fitness (VO(2max)), total and PA energy expenditure by doubly labelled water, and PA by accelerometry. Fat mass was measured by DXA. At follow-up we repeated our measurements of PA and FM.

    RESULTS: Fat mass increased significantly (P<0.001) in both groups. Physical activity did not change between baseline and 'follow up'. Change in overall PA (counts per minute) was inversely associated with change in BW and (beta=-0.0124, P=0.054) and FM (beta=-0.008, P=0.04). Post hoc analyses suggested that this association was explained by changes in the normal weight group only (beta=-0.01; P=0.008; and beta=-0.0097; P=0.009, for BW and FM, respectively). Metabolic determinants, energy expenditure estimates and subcomponents of PA (i.e. time spent at different intensity levels) were not significantly associated with change in BW or FM.

    CONCLUSION: Our results suggest an independent association between PA and FM. However, this association may differ depending on obesity status. The gain in FM, without any change in PA, may suggest that dietary intake is the major contributor to the positive energy balance.

  • 79.
    Eklund, Daniel
    et al.
    Division of Microbiology and Molecular Medicine, Linköping University, Linköping, Sweden.
    Welin, Amanda
    Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
    Andersson, Henrik
    Division of Microbiology and Molecular Medicine, Linköping University, Linköping, Sweden.
    Verma, Deepti
    Division of Cell Biology, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
    Söderkvist, Peter
    Division of Cell Biology, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
    Stendahl, Olle
    Division of Microbiology and Molecular Medicine, Linköping University, Linköping, Sweden.
    Särndahl, Eva
    Örebro universitet, Institutionen för läkarutbildning.
    Lerm, Maria
    Division of Microbiology and Molecular Medicine, Linköping University, Linköping, Sweden.
    Human Gene Variants Linked to Enhanced NLRP3 Activity Limit Intramacrophage Growth of Mycobacterium tuberculosis2014Ingår i: Journal of Infectious Diseases, ISSN 0022-1899, E-ISSN 1537-6613, Vol. 209, nr 5, s. 749-753Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Activation of the NLRP3 inflammasome and subsequent generation of interleukin 1 beta is initiated in macrophages upon recognition of several stimuli. In the present work, we show that gain-of-function gene variants of inflammasome components known to predispose individuals to inflammatory disorders have a host-protective role during infection with Mycobacterium tuberculosis. By isolation of macrophages from patients and healthy blood donors with genetic variants in NLRP3 and CARD8 and subsequent infection of the cells with virulent M. tuberculosis, we show that these gene variants, combined, are associated with increased control of bacterial growth in human macrophages.

  • 80.
    Eklund, Michael
    Örebro universitet, Institutionen för läkarutbildning.
    Proliferative and Migratory Capabilities of Fibroblasts in Dupuytren’s Disease2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 81.
    Ekström, Linnea
    et al.
    Karolinska Institute, Stockholm, Sweden.
    Adolfsson, Annsofie
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Ericson, Henrik
    Högskolan i Skövde, Skövde, Sweden.
    Poutakidis, Georgios
    Skaraborgs Sjukhus, Skövde, Sweden.
    Charonis, Georgios
    Larsson, Per-Göran
    Örebro universitet, Institutionen för läkarutbildning.
    Vaginal flora and urinary and vaginal group B streptococci in early pregnancy2013Ingår i: Gynecology, ISSN 2052-6210, Vol. 1Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Bacterial vaginosis (BV) is a risk factor for premature birth and group B streptococci (GBS) colonizing the vagina are etiological agents of neonatal infections. Significant growth of GBS in the vagina has been assumed to be detectable through urinary culture. The aim was to investigate the correlation between BV and the presence of GBS in qualitative vaginal or quantitative urinary culture, since this could predict a higher risk for perinatal morbidity.

    Design and setting: A consecutive prospective study of women in early pregnancy included 3101 women between 2007 and 2010, in a region of south-western Sweden.

    Methods: Vaginal and urine samples were obtained from women in early pregnancy at maternity health care clinics. BV was diagnosed according to the Ison/Hay classification. GBS in urine were detected in amounts as low as 100 CFU/ml. Vaginal culturing for GBS was obtained from a selected group of 481 women.

    Results: There was no difference in the prevalence of GBS in the urine among women with BV compared with women with lactobacilli flora (OR 0.7; 95% CI 0.4-1.1). Vaginal presence of GBS was found among 17.3% of women with BV and among 23.5% of women with lactobacilli flora (OR 0.7; 95% CI 0.3-1.4). Among the 105 women who had vaginal GBS, the urine culture of GBS was positive in only 21.9% of cases.

    Conclusions: Even though women with BV. have much higher concentration of bacteria in the vagina, they do not necessarily have more GBS in the vagina or urine. The modest correlation between positive vaginal culture and positive urine culture of GBS question the value of urinary culture for detection of vaginal GBS.

  • 82.
    Elfsten, Frida
    Örebro universitet, Institutionen för läkarutbildning.
    Trends in body mass index among adolescents with type 1 diabetes. A 21-year retrospective population-based study.2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 83.
    Elfving, Adam
    Örebro universitet, Institutionen för läkarutbildning.
    Follow up Study on Chest Drain-or Surgically Treated Pneumothorax Patients2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 84.
    Elgbratt, Kristina
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Jansson, Andreas
    Systems Biology Research Centre, University of Skövde, Skövde, Sweden.
    Hultgren-Hörnquist, Elisabeth
    Örebro universitet, Institutionen för läkarutbildning.
    A quantitative study of the mechanisms behind thymic atrophy in G alpha i2-deficient mice during colitis development2012Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, nr 5, artikel-id e36726Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Mice deficient for the G protein subunit G alpha i2 spontaneously develop colitis, a chronic inflammatory disease associated with dysregulated T cell responses. We and others have previously demonstrated a thymic involution in these mice and an aberrant thymocyte dynamics. The G alpha i2(-/-) mice have a dramatically reduced fraction of double positive thymocytes and an increased fraction of single positive (SP) thymocytes. In this study, we quantify a number of critical parameters in order to narrow down the underlying mechanisms that cause the dynamical changes of the thymocyte development in the G alpha i2(-/-) mice. Our data suggest that the increased fraction of SP thymocytes results only from a decreased number of DP thymocytes, since the number of SP thymocytes in the Gai2(-/-) mice is comparable to the control littermates. By measuring the frequency of T cell receptor excision circles (TRECs) in the thymocytes, we demonstrate that the number of cell divisions the G alpha i2(-/-) SP thymocytes undergo is comparable to SP thymocytes from control littermates. In addition, our data show that the mature SP CD4(+) and CD8(+) thymocytes divide to the same extent before they egress from the thymus. By estimating the number of peripheral TREC+ T lymphocytes and their death rate, we could calculate the daily egression of thymocytes. G alpha i2(-/-) mice with no/mild and moderate colitis were found to have a slower export rate in comparison to the control littermates. The quantitative measurements in this study suggest a number of dynamical changes in the thymocyte development during the progression of colitis.

  • 85. Ellinghaus, David
    et al.
    Zhang, Hu
    Zeissig, Sebastian
    Lipinski, Simone
    Till, Andreas
    Jiang, Tao
    Stade, Bjoern
    Bromberg, Yana
    Ellinghaus, Eva
    Keller, Andreas
    Rivas, Manuel A.
    Skieceviciene, Jurgita
    Doncheva, Nadezhda T.
    Liu, Xiao
    Liu, Qing
    Jiang, Fuman
    Forster, Michael
    Mayr, Gabriele
    Albrecht, Mario
    Haesler, Robert
    Boehm, Bernhard O.
    Goodall, Jane
    Berzuini, Carlo R.
    Lee, James
    Andersen, Vibeke
    Vogel, Ulla
    Kupcinskas, Limas
    Kayser, Manfred
    Krawczak, Michael
    Nikolaus, Susanna
    Weersma, Rinse K.
    Ponsioen, Cyriel Y.
    Sans, Miquel
    Wijmenga, Cisca
    Strachan, David P.
    McAardle, Wendy L.
    Vermeire, Severine
    Rutgeerts, Paul
    Sanderson, Jeremy D.
    Mathew, Christopher G.
    Vatn, Morten H.
    Wang, Jun
    Noethen, Markus M.
    Duerr, Richard H.
    Buening, Carsten
    Brand, Stephan
    Glas, Juergen
    Winkelmann, Juliane
    Illig, Thomas
    Latiano, Anna
    Annese, Vito
    Halfvarson, Jonas
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län.
    D'Amato, Mauro
    Daly, Mark J.
    Nothnagel, Michael
    Karlsen, Tom H.
    Subramani, Suresh
    Rosenstiel, Philip
    Schreiber, Stefan
    Parkes, Miles
    Franke, Andre
    Association between variants of PRDM1 and NDP52 and Crohn's disease, based on exome sequencing and functional studies2013Ingår i: Gastroenterology, ISSN 0016-5085, E-ISSN 1528-0012, Vol. 145, nr 2, s. 339-347Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND & AIMS: Genome-wide association studies (GWAS) have identified 140 Crohn's disease (CD) susceptibility loci. For most loci, the variants that cause disease are not known and the genes affected by these variants have not been identified. We aimed to identify variants that cause CD through detailed sequencing, genetic association, expression, and functional studies.

    METHODS: We sequenced whole exomes of 42 unrelated subjects with CD and 5 healthy subjects (controls) and then filtered single nucleotide variants by incorporating association results from meta-analyses of CD GWAS and in silico mutation effect prediction algorithms. We then genotyped 9348 subjects with CD, 2868 subjects with ulcerative colitis, and 14,567 control subjects and associated variants analyzed in functional studies using materials from subjects and controls and in vitro model systems.

    RESULTS: We identified rare missense mutations in PR domain-containing 1 (PRDM1) and associated these with CD. These mutations increased proliferation of T cells and secretion of cytokines on activation and increased expression of the adhesion molecule L-selectin. A common CD risk allele, identified in GWAS, correlated with reduced expression of PRDM1 in ileal biopsy specimens and peripheral blood mononuclear cells (combined P = 1.6 x 10(-8)). We identified an association between CD and a common missense variant, Val248Ala, in nuclear domain 10 protein 52 (NDP52) (P = 4.83 x 10(-9)). We found that this variant impairs the regulatory functions of NDP52 to inhibit nuclear factor kappa B activation of genes that regulate inflammation and affect the stability of proteins in Toll-like receptor pathways.

    CONCLUSIONS: We have extended the results of GWAS and provide evidence that variants in PRDM1 and NDP52 determine susceptibility to CD. PRDM1 maps adjacent to a CD interval identified in GWAS and encodes a transcription factor expressed by T and B cells. NDP52 is an adaptor protein that functions in selective autophagy of intracellular bacteria and signaling molecules, supporting the role of autophagy in the pathogenesis of CD.

  • 86.
    El-Sharkawy, Ahmed M.
    et al.
    Gastrointestinal Surgery, National Institute for Health Research Nottingham Digestive Diseases Biomedical Research Unit, Nottingham University Hospitals and University of Nottingham, Queen’s Medical Centre, Nottingham, UK.
    Watson, Phillip
    School of Exercise and Health Sciences, Loughborough University, Loughborough, UK.
    Neal, Keith R.
    Division of Public Health and Epidemiology, University of Nottingham, Nottingham, UK.
    Ljungqvist, Olle
    Örebro universitet, Institutionen för läkarutbildning.
    Maughan, Ron J.
    School of Exercise and Health Sciences, Loughborough University, Loughborough, UK.
    Sahota, Opinder
    Department of Elderly Medicine, Nottingham University Hospitals, Queen’s Medical Centre, Nottingham, UK.
    Lobo, Dileep N.
    Gastrointestinal Surgery, National Institute for Health Research Nottingham Digestive Diseases Biomedical Research Unit, Nottingham University Hospitals and University of Nottingham, Queen’s Medical Centre, Nottingham, UK.
    Hydration and outcome in older patients admitted to hospital (The HOOP prospective cohort study)2015Ingår i: Age and Ageing, ISSN 0002-0729, E-ISSN 1468-2834, Vol. 44, nr 6, s. 943-947Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: older adults are susceptible to dehydration due to age-related pathophysiological changes. We aimed to investigate the prevalence of hyperosmolar dehydration (HD) in hospitalised older adults, aged >= 65 years, admitted as an emergency and to assess the impact on short-term and long-term outcome.

    Methods: this prospective cohort study was performed on older adult participants who were admitted acutely to a large UK teaching hospital. Data collected included the Charlson comorbidity index (CCI), national early warning score (NEWS), Canadian Study of Health and Aging (CSHA) clinical frailty scale and Nutrition Risk Screening Tool (NRS) 2002. Admission bloods were used to measure serum osmolality. HD was defined as serum osmolality > 300 mOsmol/kg. Participants who were still in hospital 48 h after admission were reviewed, and the same measurements were repeated.

    Results: a total of 200 participants were recruited at admission to hospital, 37% of whom were dehydrated. Of those dehydrated, 62% were still dehydrated when reviewed at 48 h after admission. Overall, 7% of the participants died in hospital, 79% of whom were dehydrated at admission (P = 0.001). Cox regression analysis adjusted for age, gender, CCI, NEWS, CSHA and NRS demonstrated that participants dehydrated at admission were 6 times more likely to die in hospital than those euhydrated, hazards ratio (HR) 6.04 (1.64-22.25); P = 0.007.

    Conclusions: HD is common in hospitalised older adults and is associated with poor outcome. Coordinated efforts are necessary to develop comprehensive hydration assessment tools to implement and monitor a real change in culture and attitude towards hydration in hospitalised older adults.

  • 87.
    Emilsson, Louise
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Vårdcentralen Värmlands Nysäter, Värmlands Nysäter, Sweden.
    Carlsson, Roland
    PCI unit, Department of Cardiology, Central Hospital, Karlstad, Sweden .
    James, Stefan
    Department of Medical Sciences, Cardiology and Uppsala Clinical Research Centre, Uppsala University, Uppsala, Sweden.
    Hambraeus, Kristina
    Department of Cardiology and Clinical Research Centre, Falun Hospital, Falun, Sweden .
    Ludvigsson, Jonas F.
    Region Örebro län. Clinical Epidemiology Unit, Department of Medicine Solna, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden; Department of Pediatrics, Örebro University Hospital, Örebro, Sweden.
    Follow-up of ischaemic heart disease in patients with coeliac disease2015Ingår i: European Journal of Preventive Cardiology, ISSN 2047-4873, E-ISSN 2047-4881, Vol. 22, nr 1, s. 83-90Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Patients with coeliac disease and myocardial infarction have a more favourable atherosclerotic risk factor profile than controls with myocardial infarction (MI). Therefore, MI prognosis and treatment may differ according to coeliac status. This paper reports on the study of Swedish MI patients with and without coeliac disease (equal to villous atrophy; Marsh histopathology stage 3) based on duodenal or jejunal biopsy data. We used the Swedish Quality Register (SWEDEHEART) to identify individuals with a record of MI from 2005 to 2008 and to obtain data on medication, coronary interventions, and clinical and laboratory parameters at 6–10 weeks and one year after first MI. One-year mortality and coronary interventions were assessed for 430 coeliac patients and 1988 controls. For other outcome variables, we compared 42 coeliac patients with MI and 201 general population controls with MI. Odds ratios (ORs) were calculated by logistic regression. The results showed that compared with controls with MI, coeliac individuals with MI had significantly higher one-year all-cause mortality (OR = 1.43; 95% confidence interval (CI) = 1.04–1.95) but less often underwent a percutaneous coronary intervention (OR = 0.77; 95% CI = 0.61–0.96). Coeliac patients were more often prescribed warfarin but less often aspirin and statins. The readmission rate due to cardiac events in coeliac patients was 15.2% vs. 12.6% in controls (p-value  = 0.69). Other clinical and laboratory parameters were similar. We conclude that the follow up of MI does not seem to differ between coeliac patients and controls, and is unlikely to explain the excess mortality from cardiovascular disease noted in Swedish patients with CD.

  • 88.
    Emilsson, Louise
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Primary Care Res Unit, Värmlands Nysäter, Värmland County Council, Karlstad, Sweden.
    James, Stefan
    Department of Medical Sciences, Cardiology and Uppsala Clinical Research Center, Uppsala University, Uppsala, Sweden.
    Ludvigsson, Jonas F.
    Region Örebro län. Department of Pediatrics, Örebro University Hospital, Örebro, Sweden; Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
    Ischaemic heart disease in first-degree relatives to coeliac patients2014Ingår i: European Journal of Clinical Investigation, ISSN 0014-2972, E-ISSN 1365-2362, Vol. 44, nr 4, s. 359-364Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective: Coeliac disease (CD) has been linked to an increased risk of ischaemic heart disease (IHD). We examined the risk of IHD in first-degree relatives and spouses to coeliac patients to ascertain the genetic contribution to IHD excess risk.

    Study design and setting: Coeliac disease was defined as having a biopsy-verified villous atrophy (Marsh grade 3) in 1969-2008 (n=29096). Coeliac patients were matched to 144522 controls. Through Swedish registers, we identified all first-degree relatives and spouses to coeliac patients and their controls, in total 87622 unique coeliac relatives and 432655 unique control relatives. Our main outcome measure was IHD defined according to relevant international classification of disease codes in the Swedish Inpatient Registry or in the Cause of Death Registry. Hazard ratios (HR) and confidence intervals (CI) were estimated through Cox regression adjusted for sex, age-group and calendar year at study entry of the relative.

    Result: During a median follow-up of 108 years, 2880 coeliac relatives and 13817 control relatives experienced IHD. First-degree relatives of coeliac patients were at increased risk of IHD (HR=105; 95% CI=100-109, P-value=004), while spouses were at no increased risk (HR=099; 95% CI=087-112). The excess risk of IHD in coeliac first-degree relatives aged 40-59years was 70/100000 person-years.

    Conclusion: First-degree relatives to coeliac patients seem to be at an increased risk of IHD but the excess risk is so small that it has little clinical relevance.

  • 89.
    Emilsson, Louise
    et al.
    Örebro universitet, Institutionen för läkarutbildning. Primary Care Research Unit, Vårdcentralen Värmlands Nysäter, Värmland County, Värmlands Nysäter, Sweden; Department of Health Management and Health Economy, Institute of Health and Society, University of Oslo, Oslo, Norway.
    Lindahl, B.
    Department of Medical Sciences, Cardiology, and Uppsala Clinical Research Center, University of Uppsala, Uppsala, Sweden.
    Köster, M.
    National Board of Health and Welfare, Stockholm, Sweden .
    Lambe, M.
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; Regional Cancer Centre, Uppsala, Sweden.
    Ludvigsson, Jonas F.
    Region Örebro län. Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; Department of Paediatrics, Örebro University Hospital, Örebro, Sweden.
    Review of 103 Swedish healthcare quality registries2015Ingår i: Journal of Internal Medicine, ISSN 0954-6820, E-ISSN 1365-2796, Vol. 277, nr 1, s. 94-136Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background and objectives: In the past two decades, an increasing number of nationwide, Swedish Healthcare Quality Registries (QRs) focusing on specific disorders have been initiated, mostly by physicians. Here, we describe the purpose, organization, variables, coverage and completeness of 103 Swedish QRs.

    Methods: From March to September 2013, we examined the 2012 applications of 103 QRs to the Swedish Association of Local Authorities and Regions (SALAR) and also studied the annual reports from the same QRs. After initial data abstraction, the coordinator of each QR was contacted at least twice between June and October 2013 and asked to confirm the accuracy of the data retrieved from the applications and reports.

    Results: About 60% of the QRs covered 80% of their target population (completeness). Data recorded in Swedish QRs include aspects of disease management (diagnosis, clinical characteristics, treatment and lead times). In addition, some QRs retrieve data on self-reported quality of life (EQ5D, SF-36 and disease-specific measures), lifestyle (smoking) and general health status (World Health Organization performance status, body mass index and blood pressure).

    Conclusion: Detailed clinical data available in Swedish QRs complement information from government-administered registries and provide an important source not only for assessment and development of quality of care but also for research.

  • 90.
    Enarsson, Malin
    Örebro universitet, Institutionen för läkarutbildning.
    Investigating Mindfulness Predictive Ability on Weight Change Achieved in a Weight Loss Intervention2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 91.
    Engström, Alexander
    et al.
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Erlandsson, Ann
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Delbro, Dick
    Örebro universitet, Institutionen för läkarutbildning.
    Wijkander, Jonny
    Department of Health Sciences, Karlstad University, Karlstad, Sweden.
    Conditioned media from macrophages of M1, but not M2 phenotype, inhibit the proliferation of the colon cancer cell lines HT-29 and CACO-22014Ingår i: International Journal of Oncology, ISSN 1019-6439, Vol. 44, nr 2, s. 385-392Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Solid tumors are infiltrated by stroma cells including macrophages and these cells can affect tumor growth, metastasis and angiogenesis. We have investigated the effects of conditioned media (CM) from different macrophages on the proliferation of the colon cancer cell lines HT-29 and CACO-2. CM from THP-1 macrophages and monocyte-derived human macrophages of the M1 phenotype, but not the M2 phenotype, inhibited proliferation of the tumor cells in a dose-dependent manner. Lipopolysaccaharide and interferon gamma was used for differentiation of macrophages towards the M1 phenotype and CM were generated both during differentiation (M1(DIFF)) and after differentiation (M1). M1 and M1(DIFF) CM as well as THP-1 macrophage CM resulted in cell cycle arrest in HT-29 cells with a decrease of cells in S phase and an increase in G(2)/M phase. Treatment of HT-29 cells with M1(DIFF), but not M1 or THP-1 macrophage CM, resulted in apoptosis of about 20% of the tumor cells and this was accompanied by lack of recovery of cell growth after removal of CM and subsequent culture in fresh media. A protein array was used to identify cytokines released from M1 and M2 macrophages. Among the cytokines released by M1 macrophages, tumor necrosis factor alpha and CXCL9 were tested by direct addition to HT-29 cells, but neither affected proliferation. Our results indicate that M1 macrophages inhibit colon cancer cell growth and have the potential of contributing to reducing tumor growth in vivo.

  • 92.
    Enoksson, Oskar
    Örebro universitet, Institutionen för läkarutbildning.
    Urothelial host response to filamentous Escherichia coli2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 93.
    Erfani Torbaghani, Paniz
    Örebro universitet, Institutionen för läkarutbildning.
    The prevalence of CMV-retinitis, treatment and complications in a cohort of AIDS patients: South General Hospital, Stockholm between the years of 1989-19962014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 94.
    Ericson, Anna
    et al.
    Uppsala University, Uppsala, Sweden.
    Ställberg, Björn
    Uppsala University, Uppsala, Sweden.
    Janson, Christer
    Uppsala University, Uppsala, Sweden.
    Sundh, Josefin
    Örebro universitet, Institutionen för läkarutbildning. Department Respiratory Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Montgomery, Scott
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Kampe, Mary
    Uppsala University, Uppsala, Sweden.
    Efraimsson, Eva Osterlund
    Dalarna University, Falun, Sweden.
    Patients' evaluation on asthma severity was related to level of asthma control and quality of life over seven years follow-up2013Ingår i: European Respiratory Journal, ISSN 0903-1936, E-ISSN 1399-3003, Vol. 42, nr 57, artikel-id 2744Artikel i tidskrift (Övrigt vetenskapligt)
  • 95.
    Eriksson, Diana
    Örebro universitet, Institutionen för läkarutbildning.
    Changes in ghrelin levels following Roux-en-Y gastric bypass surgery - A systematic literature review2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 96.
    Eriksson, Jonna Maria
    et al.
    Center of Neurodevelopmental Disorders (KIND), Karolinska Institutet, Stockholm, Sweden.
    Lundström, Sebastian
    Centre for Ethics, Law and Mental Health (CELAM), University of Gothenburg, Gothenburg, Sweden; Gillberg Neuropsychiatry Centre, University of Gothenburg, Gothenburg, Sweden.
    Lichtenstein, Paul
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
    Bejerot, Susanne
    Örebro universitet, Institutionen för läkarutbildning. Center of Neurodevelopmental Disorders (KIND), Karolinska Institutet, Stockholm, Sweden.
    Eriksson, Elias
    Department of Pharmacology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
    Effect of co-twin gender on neurodevelopmental symptoms: a twin register study2016Ingår i: Molecular Autism, ISSN 2040-2392, Vol. 7, artikel-id 8Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) are neurodevelopmental disorders thought to have both genetic and environmental causes. It has been hypothesized that exposure to elevated levels of prenatal testosterone is associated with elevated traits of ASD and ADHD. Assuming that testosterone levels from a dizygotic male twin fetus may lead to enhanced testosterone exposure of its co-twins, we aimed to test the prenatal testosterone hypothesis by comparing same-sex with opposite-sex dizygotic twins with respect to neurodevelopmental symptoms.

    Methods: Neuropsychiatric traits were assessed in a population-based twin cohort from the Child and Adolescent Twin Study in Sweden (CATSS). Parental interviews were conducted for 16,312 dizygotic twins, 9 and 12 years old, with the Autism-Tics, ADHD, and other Comorbidities inventory (A-TAC).

    Results: Girls with a female co-twin had an increased risk of reaching the cut-off score for ADHD compared with girls with a male co-twin. Both boys and girls with a female co-twin displayed a larger number of traits related to attention deficit and repetitive and stereotyped behaviors than those with a male twin. In girls, this also extended to social interaction and the combined measures for ASD and ADHD, however, with small effect sizes.

    Conclusions: Our results are reverse to what would have been expected from the prenatal testosterone hypothesis but consistent with a previous study of ASD and ADHD traits in dizygotic twins. The seemingly protective effect for girls of having a twin brother may be an effect of parent report bias, but may also be an unexpected effect of sharing the intrauterine environment with a male co-twin.

  • 97.
    Esmail, Narmin-Katarina
    Örebro universitet, Institutionen för läkarutbildning.
    Risks of worsening of Non-alcoholic Fatty Liver Disease (NAFLD) after bariatric surgery: – a systematic literature review2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 98.
    Eunyoung, Cho
    et al.
    Channing Laboratory, Department of Medicine, Harvard Medical School, Brigham and Women’s Hospital; Department of Nutrition, Harvard School of Public Health, Boston, MA, USA.
    Lindblad, Per
    Örebro universitet, Institutionen för läkarutbildning. Department of Urology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
    Adami, Hans-Olov
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; and Department of Epidemiology, Harvard School of Public Health, Boston, MA, USA.
    Kidney Cancer2008Ingår i: Textbook of cancer epidemiology / [ed] Hans-Olov Adam, David J. Hunter, Dimitrios Trichopoulos, Oxford, United Kingdom: Oxford University Press , 2008, 2nd ed.Kapitel i bok, del av antologi (Övrigt vetenskapligt)
    Abstract [en]

    Cigarette smoking and obesity may account for approximately 40% of cases in high-incidence countries. Continued research in kidney cancer is needed since nearly 50% of the patients die within five years after diagnosis. With the aim of prevention, the continued search for environmental causes should take into account the fact that kidney cancer consists of different types with specific genetic molecular characteristics. In some cases, these genetic alterations have been purportedly associated with specific exposures. Furthermore, genetic polymorphisms may have a modulating effect on metabolic activation and detoxification enzymes. Thus, better understanding of the genetic and molecular processes involved in kidney cancer may help with the analyzing exposure associations that are important in both its initiation and progression.

  • 99.
    Falk-Brynhildsen, Karin
    et al.
    Örebro universitet, Institutionen för hälsovetenskap och medicin. Department of Cardiothoracic and Vascular Surgery, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
    Söderquist, Bo
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Laboratory Medicine, Clinical Microbiology ,Örebro University Hospital, Örebro, Sweden.
    Friberg, Örjan
    Region Örebro län. Department of Cardiothoracic and Vascular Surgery, Örebro University Hospital, Örebro, Sweden.
    Nilsson, Ulrica
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Bacterial growth and wound infection following saphenous vein harvesting in cardiac surgery: a randomized controlled trial of the impact of microbial sealant2014Ingår i: European Journal of Clinical Microbiology and Infectious Diseases, ISSN 0934-9723, E-ISSN 1435-4373, Vol. 33, nr 11, s. 1981-1987Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The aim of the present study was to compare microbial skin sealant versus bare skin on the leg regarding intraoperative bacterial presence in the surgical wound and time to recolonization of the adjacent skin at the saphenous vein harvesting site. A second aim was to evaluate the incidence of leg wound infection 2 months after surgery. In this randomized controlled trial, 140 patients undergoing coronary artery bypass grafting (CABG) between May 2010 and October 2011 were enrolled. Bacterial samples were taken preoperatively and intraoperatively at multiple time points and locations. OF the patients, 125 (92.6 %) were followed up 2 months postoperatively regarding wound infection. Intraoperative bacterial growth did not differ between the bare skin (n = 68) and the microbial skin sealant group (n = 67) at any time point. At 2 months postoperatively, 7/61 patients (11.5 %) in the skin sealant versus 14/64 (21.9 %) in the bare skin group (p = 0.120) had been treated with antibiotics for a verified or suspected surgical site infection (SSI) at the harvest site. We found almost no intraoperative bacterial presence on the skin or in the subcutaneous tissue, irrespective of microbial skin sealant use. In contrast, we observed a relatively high incidence of late wound infection, indicating that wound contamination occurred postoperatively. Further research is necessary to determine whether the use of microbial skin sealant reduces the incidence of leg wound infection at the saphenous vein harvest site.

  • 100.
    Fall, Sofia
    et al.
    Department of Dermatology, Örebro University Hospital, Örebro, Sweden .
    Bruze, Magnus
    Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden .
    Isaksson, Marlene
    Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden .
    Lidén, Carola
    Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden .
    Matura, Mihaly
    Unit of Occupational and Environmental Dermatology, Centre for Occupational and Environmental Medicine, Stockholm County Council, Stockholm, Sweden .
    Stenberg, Berndt
    Dermatology and Venereology, Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden.
    Lindberg, Magnus
    Örebro universitet, Institutionen för läkarutbildning. Region Örebro län. Department of Dermatology, Örebro University Hospital, Örebro, Sweden.
    Contact allergy trends in Sweden: a retrospective comparison of patch test data from 1992, 2000, and 20092015Ingår i: Contact Dermatitis, ISSN 0105-1873, E-ISSN 1600-0536, Vol. 72, nr 5, s. 297-304Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Contact allergy prevalence rates change over time as a result of variations in allergen exposure. Data from patch test clinics are often used as markers for allergy trends.

    Objectives: The aim of the present retrospective study was to describe trends in rates of sensitization to allergens in the Swedish baseline series.

    Patients/materials/methods: Prevalence rates are described by comparing consecutive patch test data from 1992, 2000 and 2009 in Swedish patch test clinics. In total, 3680 patients were included in 1992, 3825 in 2000, and 3112 in 2009.

    Results: Among test substances with a sensitization rate above 2% in 2009, significant decreases were noted for nickel sulfate, cobalt chloride, colophonium, and methylchloroisothiazolinone (MCI)/methylisothiazolinone (MI), and a significant increase for p-phenylenediamine, as compared with 1992. Potassium dichromate reactions had increased among younger women, whereas reactions to nickel and cobalt had decreased in this group. Sensitization to chromium, cobalt and fragrance mix I had decreased among older men, and sensitization to nickel had decreased among younger men.

    Conclusions: It is probable that these changes in 1992-2009 reflect both changes in regulations for nickel, lower levels of chromium in cement and of MCI/MI in cosmetics, and increasing use of hair dyes.

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