Antibiotic resistance is now a global challenge. Antibiotic resistance genes (ARGs) have been detected in environments directly related to various sources of antibiotic outflow, such as wastewater treatment plants and livestock facilities. The study of ARGs in wild species living in the surroundings of anthropogenic activities brings knowledge in the transmission into wildlife and the potential reservoirs, harbouring the evolution of ARGs. This project has focused on detecting ARGs in the gut microbiota of wild boars (Sus scrofa) in areas where human activities and wildlife interface, comparing to low human-activity areas. The collection of wild boar-fecal samples were achieved in collaboration with local hunters in a south-centre region of Sweden, and the locations of the habitats were categorized by its surrounding profile of anthropogenic activities. The presence of ARGs were determined by purifying the DNA and search for the presence of ARGs by real-time qPCR. The results emphasized that the wild boars in close range of human activities, facilitated a higher presence of the tested ARGs than individuals from remote areas. Clinically important genes such as, aad1A, qnrB and vanC were found in the wild boars surrounding livestock facilities and agricultural lands. The results also show that there are differences in the diversity of ARGs present in wild boar intestines in the analysed areas. Interestingly, the result also reflects the importance of the agriculture areas in the dissemination of ARGs, suggesting that they should be taken into consideration in further plans to reduce ARGs spreading in the environment. This is the first study to our knowledge that is studying wild boars as a reservoir of antimicrobial resistance in Sweden.