Despite significant investments in promoting entrepreneurship, there has been a decline in the number of successful innovative firms in Europe and North America. Universities are crucial for transforming research into commercially viable innovations, and various support ecosystems have emerged to facilitate this process. This study investigates the early-stage activities at Technology Transfer Offices (TTOs) and incubators within university innovation support ecosystems, exploring the potential misalignments and inconsistencies that may hinder the overarching goal of commercializing academic knowledge. Employing a qualitative case study method, we conducted an in-depth examination of the Swedish academic entrepreneurial ecosystem. Our findings indicate the presence of tensions, conflicts, and contradictions among different stakeholders, each with their unique interests, values, and perspectives. This study contributes to the understanding of these complex relationships and emphasizes the importance of aligning goals and fostering collaboration within the academic ecosystem to effectively commercialize university research.